بررسی برون تنی عصاره‌ی هیدروالکلی سیر وحشی (Allium ampeloprasum) بر فعالیت حرکتی ایلئوم موش صحرایی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 کارشناس ارشد، مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

2 دانشجوی پزشکی، مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

3 استاد، گروه نفرولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

4 کارشناس ارشد، گروه آمار، دانشکده‌ی بهداشت، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

5 استاد، مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

چکیده

مقدمه: در این تحقیق، اثر ضد انقباضی عصاره‌ی هیدروالکلی برگ گیاه تره‌ی کوهی بر انقباضات ایلئوم موش‌های صحرایی نژاد ویستار و مکانیسم محتمل بر آن بررسی شد.روش‌ها: در این تحقیق مداخله‌ای تعداد 48 سر موش صحرایی، از نژاد ویستار به طور تصادفی به شش گروه هشت‌تایی به شرح زیر تقسیم شدند: شاهد، دریافت کننده‌ی غلظت‌های تجمعی عصاره‌ی تره‌ی کوهی، دریافت کننده‌ی L-NAME (L-NG-nitroarginine methyl ester) و عصاره، نالوکسان و عصاره، پروپرانولول و عصاره و گروه دریافت کننده‌ی غلظت‌های تجمعی کلرور کلسیم. در روز آزمایش، ایلئوم موش‌ها جدا و انقباضات آن در حمام بافت تحت یک گرم کشش با اضافه کردن کلرور پتاسیم (mM 60) به روش ایزوتونیک ثبت شد. جهت بررسی مکانیسم اثر عصاره، بافت با پروپرانول، نالوکسان و یا L-NAME انکوبه شد و درصد تغییرات نیروی انقباضی بر روی کاغذ ثبت و محاسبه گردید. همچنین جهت نقش کانال‌های کلسیم در فعالیت حرکتی بافت، ایلئوم تحت تأثیر غلظت‌های تجمعی کلرور کلسیم قرار داده شد.یافته‌ها: عصاره‌ی تجمعی تره‌ی کوهی (100، 200 و 400 میلی‌گرم بر کیلوگرم) به صورت وابسته به غلظت انقباضات ایلئوم به وسیله‌ی کلرور پتاسیم را کاهش داد (0001/0 > P). پروپرانولول اثر مهاری عصاره بر انقباضات ناشی از کلرور پتاسیم را به صورت معنی‌داری کاهش داد (0010/0 > P). اما، L-NAME و نالوکسان سبب کاهش اثر مهاری عصاره بر ایلئوم نشدند. همچنین کلسیم سبب انقباض بافت دپولاریزه شده توسط کلرور پتاسیم شد و این اثر انقباضی توسط غلظت‌های تجمعی عصاره به طور معنی‌داری کاهش یافت (0010/0 > P).نتیجه‌گیری: عصاره‌ی هیدروالکلی برگ تره‌ی کوهی توانست با اثر بر گیرنده‌های بتاآدرنرژیک و کانال‌های کلسیم وابسته به ولتاژ، بر فعالیت حرکتی ایلئوم موش صحرایی اثر بگذارد و با توجه به نتیجه‌ی اثر، ممکن است بتوان از آن در درمان ناراحتی‌های گوارش استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions

نویسندگان [English]

  • Masoumeh Sedighi-Hafshejani 1
  • Mosayyeb Noori-Ahmadabadi 2
  • Hamid Nasri 3
  • Molok Hadi 4
  • Mahmoud Rafieian-Kopaei 5
1 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Student of Medicine, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Professor, Department of Nephrology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Statistics, School of Health, Shahrekord University of Medical Sciences, Shahrekord, Iran
5 Professor, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
چکیده [English]

Background: Ampeloprasum is traditionally used for gastrointestinal disorders. In this experimental study, the effect of ethanolic extract of Allium ampeloprasum was investigated on the rat ileum contractions. The mechanisms were also evaluated.Methods: In this experimental study, 48 male Wistar rats were randomly designated into six equal groups, including control and cumulative concentrations of Allium ampeloprasum, propranolol, naloxone, L-name, and CaCl2. In the day of the experiment, the ileum was dissected and its contractions were recorded in Tyrode solution in an organ bath. To evaluate the mechanisms involved, propranolol, naloxone, or L-name was incubated and the percent of changes in contractions were recorded.Findings: The extract of Allium ampeloprasum dose-dependently reduced the contractions induced by KCl (60 mM) (P < 0.0001); propranolol reversed this effect (P < 0.0010), but L-name and naloxone were not effective. CaCl2 increased the contractions (P < 0.0010) and the extract reversed this effect.Conclusion: Allium ampeloprasum leaves can affect the ileum contractions through beta adrenoreceptors and voltage-dependent calcium channels and might be used in gastrointestinal disorders.

کلیدواژه‌ها [English]

  • Allium ampeloprasum
  • Ileum
  • Beta-blockers

مراجع

  1. Rafieian-Kopaei M. Medicinal plants and the human needs. J HerbMed Pharmacol 2012; 1(1): 1-2.
  2. Asgari A. Herbal medicines and kidney; friends or foes? J Nephropharmacol 2014; 3(1): 5-6.
  3. Rafieian-Kopaei M, Baradaran A, Rafieian M. Plants antioxidants: From laboratory to clinic. J Nephropathol 2013; 2(2): 152-3.
  4. Nasri H, Rafieian-Kopaei M. Herbal medicine and diabetic kidney disease. J Nephropharmacol 2013; 2(1): 1-2.
  5. Shirzad H, Nasri H. Toxicity and safety of medicinal plants. J Herbmed Pharmacol 2013; 2(2): 1-2.
  6. Behradmanesh S, Derees F, Rafieian-Kopaei M. Effect of Salvia officinalis on diabetic patients. J Ren Inj Prev 2013; 2(2): 51-4.
  7. Asadi SY, Parsaei P, Karimi M, Rafieian-Kopaei M. Effect of ethanolic extract of Green tea (Camellia sinensis) on intra-abdominal adhesions in rats. J Zanjan Univ Med Sci 2013; 21(86): 86-96. [In Persian].
  8. Kazemi S, Asgary S, Moshtaghian J, Rafieian M, Adelnia A, Shamsi F. Liver-protective effects of hydroalcoholic extract of Allium hirtifolium boiss. In rats with alloxan-induced diabetes mellitus. ARYA Atheroscler 2010; 6(1): 11-5.
  9. Hajivandi A, Amiri M. World Kidney Day 2014: Kidney disease and elderly. J Parathyroid Dis 2014; 2(1):3-4.
  10. Ardalan MR, Rafieian-Kopaei M. Is the safety of herbal medicines for kidneys under question? J Nephropharmacol 2013; 2(2): 5-6.
  11. Bahmani M, Vakili-Saatloo N, Gholami-Ahangaran M, Karamati SA, Khalil-Banihabib E, Hajigholizadeh Gh, et al. A comparison study on the anti-leech effects of onion (Allium cepa L) and ginger (Zingiber officinale) with levamisole and triclabendazole. J HerbMed Pharmacol 2013; 2(1): 1-3.
  12. Eidi M, Soleimani F, Ebrahimi S. Hypolipidemic Effects of Allium porrum L. Leaves in Healthy and Streptozotocin-Induced Diabetic Mice. Journal of Medicinal Plants 2007; 6(24): 8-13.
  13. Mozafrian V. Culture of plant names. Tehran, Iran: Farhang Moaser Publication; 2003. p. 27. [In Persian].
  14. Nguansangiam S, Angsubhakorn S, Bhamarapravati S, Suksamrarn A. Effects of elephant garlic volatile oil (Allium ampeloprasum) and T-2 toxin on murine skin. Southeast Asian J Trop Med Public Health 2003; 34(4): 899-905.
  15. Morita T, Ushiroguchi T, Hayashi N, Matsuura H, Itakura Y, Fuwa T. Steroidal saponins from elephant garlic, bulbs of Allium ampeloprasum L. Chem Pharm Bull (Tokyo) 1988; 36(9): 3480-6.
  16. Bown D. Encyclopedia of Herbs and Their Uses. New York, NY: Reader's Digest Association p. 20-31; 1995.
  17. Khalaji S, Eidi M, Eidi A, Shahmohammadi P, Arab M. Hyperalgesia effect of leaf Allium ampeloprasum extract attenuates (Allium ampeloprasum l. subsp. Iranicum) in normal and diabetic mice. Iranian Journal of Biological Scienses 2009; 4(1): 9-14. [In Persian].
  18. Kadkhodaee M, Sedaghat Z. Novel renoprotection methods by local and remote conditioning. J Ren Inj Prev 2014; 3(2): 37-8.
  19. Tavafi M. Complexity of diabetic nephropathy pathogenesis and design of investigations. J Ren Inj Prev 2013; 2(1): 59-62.
  20. Augusti KT. Therapeutic values of onion (Allium cepa L.) and garlic (Allium sativum L.). Indian J Exp Biol 1996; 34(7): 634-40.
  21. Ardalan MR, Rafieian-Kopaie M. Antioxidant supplementation in hypertension. J Ren Inj Prev 2014; 3(2): 39-40.
  22. Nasri H, Behradmanesh S, Ahmadi A, Baradaran A, Nasri P, Rafieian-Kopaei M. Association of serum lipids with level of blood pressure in type 2 diabetic patients. J Ren Inj Prev 2014; 3(2): 43-6.
  23. Eidi A, Eidi M, Esmaeili E. Antidiabetic effect of garlic (Allium sativum L.) in normal and streptozotocin-induced diabetic rats. Phytomedicine 2006; 13(9-10): 624-9.
  24. Tamadon MR, Baradaran A, Rafieian-Kopaei M. Antioxidant and kidney protection; differential impacts of single and whole natural antioxidants. J Ren Inj Prev 2014; 3(2): 41-2.
  25. Nasri H, Ahmadi A, Baradaran A, Nasri P, Hajian S, Pour-Arian A, et al. A biochemical study on ameliorative effect of green tea (Camellia sinensis) extract against contrast media induced acute kidney injury. J Ren Inj Prev 2014; 3(1): 47-9.
  26. Liu CT, Hse H, Lii CK, Chen PS, Sheen LY. Effects of garlic oil and diallyl trisulfide on glycemic control in diabetic rats. Eur J Pharmacol 2005; 516(2): 165-73.
  27. Mardani S, Nasri H, Hajian S, Ahmadi A, Kazemi R, Rafieian-Kopaei M. Impact of Momordica charantia extract on kidney function and structure in mice. J Nephropathol 2014; 3(1): 35-40.
  28. Nasri H, Rafieian-Kopaei M. Metformin improves diabetic kidney disease. J Nephropharmacol 2012; 1(1): 1-2.
  29. Roghani M, Aghaie M. The effect of Allium ampeloprasum on nociceptive response intensity in diabetic rats. J Gorgan Uni Med Sci 2007; 9(3): 10-4. [In Persian].
  30. Kumari K, Augusti KT. Antidiabetic and antioxidant effects of S-methyl cysteine sulfoxide isolated from onions (Allium cepa Linn) as compared to standard drugs in alloxan diabetic rats. Indian J Exp Biol 2002; 40(9): 1005-9.
  31. Kumari K, Mathew BC, Augusti KT. Antidiabetic and hypolipidemic effects of S-methyl cysteine sulfoxide isolated from Allium cepa Linn. Indian J Biochem Biophys 1995; 32(1): 49-54.
  32. Fritsch RM, Keusgen M. Occurrence and taxonomic significance of cysteine sulphoxides in the genus Allium L. (Alliaceae). Phytochemistry 2006; 67(11): 1127-35.
  33. Ratz PH, Berg KM, Urban NH, Miner AS. Regulation of smooth muscle calcium sensitivity: KCl as a calcium-sensitizing stimulus. Am J Physiol Cell Physiol 2005; 288(4): C769-C783.
  34. Ratz RH, Berg KM, Urban NH, Miner AS. Role of Ca2+ and MLK in regulation of smooth muscle. Am J Physiol 2005; 248: 2769-83.
  35. Roghani M, Baluchnejadmojarad T, Ogbi K. Survey the Effect of Feeding of Allium Latifolium on Contractile Reactivity of Aorta of Diabetic Rats. J Guilan Univ Med Sci 2008; 17(65): 1-6. [In Persian].
  36. Bahmani M, Vakili-Saatloo N, Maghsoudi R, Momtaz H, Saki K, Kazemi-Ghoshchi B, et al. A comparative study on the effect of ethanol extract of wild Scrophularia deserti and streptomycin on Brucellla melitensis. J HerbMed Pharmacol 2013; 2(1): 17-20.
  37. Nasri H, Sahinfard N, Rafieian M, Rafieian S, Shirzad M, Rafieian-Kopaei M. Effects of Allium sativum on liver enzymes and atherosclerotic risk factors. J HerbMed Pharmacol 2013; 2(2): 23-8.
  38. Shirzad H, Taji F, Rafieian-Kopaei M. Correlation between antioxidant activity of garlic extracts and WEHI-164 fibrosarcoma tumor growth in BALB/c mice. J Med Food 2011; 14(9): 969-74.
  39. Ansari R, Shahinfard N, Namjou A, Rafieian M, Shirzad H, Rafieian-Kopaei M. Ameliorative property of Teucrium polium on second degree burn. J HerbMed Plarmacol 2013; 2(1): 9-11.
  40. Asgary S, Kelishadi R, Rafieian-Kopaei M, Najafi S, Najafi M, Sahebkar A. Investigation of the lipid-modifying and antiinflammatory effects of Cornus mas L. supplementation on dyslipidemic children and adolescents. Pediatr Cardiol 2013; 34(7): 1729-35.
  41. Sedighi M, Rafieian-Kopaei M, Noori-Ahmadabadi M. Efeect of Allium ampeloprasum on ileum function: Involopersum of beta-adrenergic receptors andvoltage depend calcium channels. Life Sci J 2012; 9(4): 1660-6.
  42. Nasri H, Behradmanesh S, Ahmadi A, Rafieian-Kopaei M. Impact of oral vitamin D (cholecalciferol) replacement therapy on blood pressure in type 2 diabetes patients; a randomized, double-blind, placebo controlled clinical trial. J Nephropathol 2014; 3(1): 29-33.
  43. Sedighi M, Rafieian-Kopaei M, Noori-Ahmadabadi M. Kelussia odoratissima Mozaffarian inhibits ileum contractions through voltage dependent and beta adrenergic receptors. Life Sci J 2012; 9(4): 1033-8.
  44. Rafieian-Kopaei M, Sewell RD. Newer antidepressants: analgesia and relative monoamine reuptake inhibitor potency. Pharmaceutical Journal 1994; 46(Suppl): 1088.
  45. Bolton TB. Mechanisms of action of transmitters and other substances on smooth muscle. Physiol Rev 1979; 59(3): 606-718.
  46. Guyton AC, Hall JE. Medical physiology. 10th ed. Philadelphia, PA: WB Saunders Co; 2000.
  47. El Bardai S, Lyoussi B, Wibo M, Morel N. Comparative study of the antihypertensive activity of Marrubium vulgare and of the dihydropyridine calcium antagonist amlodipine in spontaneously hypertensive rat. Clin Exp Hypertens 2004; 26(6): 465-74.
  48. Gray AC, White PJ, Coupar IM. Characterisation of opioid receptors involved in modulating circular and longitudinal muscle contraction in the rat ileum. Br J Pharmacol 2005; 144(5): 687-94.
  49. van Der V, Rademaker B, Bast A. A beta adrenoceptor with atypical characteristics is involved in the relaxation of the rat small intestine. J Pharmacol Exp Ther 1990; 255(1): 218-26.
  50. Bates DO, Hillman NJ, Williams B, Neal CR, Pocock TM. Regulation of microvascular permeability by vascular endothelial growth factors. J Anat 2002; 200(6): 581-97.
  51. Nayer A, Ortega LM. Catastrophic antiphospholipid syndrome: a clinical review. J Nephropathol 2014; 3(1): 9-17.
  52. Mubarak M, Nasri H. What nephrolopathologists need to know about antiphospholipid syndrome-associated nephropathy: Is it time for formulating a classification for renal morphologic lesions? J Nephropathol 2014; 3(1): 4-8.
  53. Kanada A, Hata F, Suthamnatpong N, Maehara T, Ishii T, Takeuchi T, et al. Key roles of nitric oxide and cyclic GMP in nonadrenergic and noncholinergic inhibition in rat ileum. Eur J Pharmacol 1992; 216(2): 287-92.
  54. Rafieian-Kopaei M, Nasri H. Vitamin D therapy in diabetic kidney disease. J Nephropharmacol 2014; 3(1): 3-4.
  55. Nasri H. Antiphospholipid syndrome-associated nephropathy: current concepts. J Ren Inj Prev 2012; 2(1): 1-2.
  56. Baradaran A. Antiphospholipid syndrome-associated nephropathy; a nephropathy needs classification. J Nephropharmacol 2012; 1(1): 9-11.
  57. Carotenuto A, De Feo V, Fattorusso E, Lanzotti V, Magno S, Cicala C. The flavonoids of Allium ursinum. Phytochemistry 1996; 41(2): 531-6.
  58. Heidarian E, Rafieian-Kopaei M, Ashrafi K. The Effect of hydroalcoholic extract of allium latifolium on the liver phosphatidate phosphatase and serum lipid profile in hyperlipidemic rats. J Babol Univ Med Sci 2013; 15(4): 37-46. [In Persian].
  59. Parsaei P, Karimi M, Asadi SY, Rafieian-Kopaei M. Bioactive components and preventive effect of green tea (Camellia sinensis) extract on post-laparotomy intra-abdominal adhesion in rats. Int J Surg. 2013; 11(9): 811-5.
  60. Tavafi M. Diabetic nephropathy and antioxidants. J Nephropathol 2013; 2(1): 20-7.
  61. Asadi SY, Parsaei P, Karimi M, Ezzati S, Zamiri A, Mohammadizadeh F, Rafieian-Kopaei M. Effect of green tea (Camellia sinensis) extract on healing process of surgical wounds in rat. Int J Surg 2013; 11(4): 332-7.
مجله دانشکده پزشکی اصفهان
دوره 31، شماره 268 - شماره پیاپی 268
بهمن و اسفند 1392
صفحه 2238-2249

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  • تاریخ دریافت: 20 تیر 1392
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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