The Relationship between POU5F1 Transcription Factor Expression and Age Increasing as an Epigenetic Factor

Document Type : Original Article(s)

Authors

1 MSc Student, Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran AND Associate Professor, Department of Biotechnology, Amol University of Special Modern Technologies 49767, Amol, Iran

2 MSc Student of Genetics, Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

3 Associate Professor, Department of Biotechnology, Amol University of Special Modern Technologies 49767, Amol, Iran

Abstract

Background: The purpose of this study is to investigate the relationship between the expression level of the important transcription factor POU5F1 in spermatogonial stem cells with age and to present the expression patterns of this factor in the mouse spermatogenic tube.
Methods: In the present study, spermatogonial stem cells were isolated after extracting the testicles of two-week-old and sixteen-week-old adult mice purchased from the Pasteur Institute of Iran. The cells were cultured in a StemPro-34 medium containing specific growth factors. Also, after fixing and preparing the tissue, they were incubated with primary and secondary antibodies, and an immunohistochemical examination was done with a confocal microscope. Then, real-time PCR analysis was used to quantitatively investigate the expression of the POU5F1 transcription factor.
Findings: While in the immunohistochemical analysis, high expression of the POU5F1 transcription factor was observed in the infant's testis, the number of POU5F1 positive cells in the seminiferous tubules of the adult testis was higher than that of the infant. Also, real-time PCR analysis showed that the level of POU5F1 gene expression in newborn SSCs was significantly (P < 0.05) higher than 16-week-old SSCs.
Conclusion: The results can reveal the foundation of new dimensions in the relationship between epigenetic factors and the differentiation power of stem cells, which should be considered in research on the differentiation of spermatogonial stem cells into sperm cells.

Highlights

Mehdi Mehdinezhad Roshan: PubMed

Hossein Azizi: Google Scholar, PubMed

Keywords

Main Subjects

References

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Journal of Isfahan Medical School
Volume 41, Issue 741
1st Week, January
January and February 2024
Pages 965-970

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History

  • Receive Date: 07 September 2023
  • Accept Date: 05 December 2023

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