بررسی ارتباط پلی‌مورفیسم در ژن Xrcc3 با سرطان‌ کولورکتال: یک مطالعه‌ی مورد- شاهدی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشیار، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه اصفهان، اصفهان، ایران

2 دانشجوی دکتری، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه اصفهان، اصفهان، ایران

3 دانشجوی کارشناسی ارشد، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه اصفهان، اصفهان، ایران

چکیده

مقدمه: سرطان‌ کولورکتال، سومین سرطان منجر به مرگ در کشورهای ‌غربی محسوب می‌شود. سن بالا، چاقی، بی‌تحرکی، رژیم غذایی نامناسب و تغییرات ژنتیکی، از‌ جمله عوامل خطر بروز این سرطان به‌ شمار می‌رود. بررسی ارتباط تغییرات ژنتیکی با سرطان ‌کولورکتال، زمینه‌ی بسیاری ‌از تحقیقات اخیر بوده و پلی‌مورفیسم M241T در ژن Xrcc3 مورد توجه قرار گرفته ‌است.روش‌ها: در این پژوهش مورد- شاهدی، 90 بیمار مبتلا به سرطان کولورکتال و 83 فرد سالم مورد مطالعه قرار گرفتند. DNA ژنومی از خون محیطی افراد استخراج و توزیع ژنوتیپی در محل پلی‌مورفیسم مذکور بررسی گردید. منطقه‌ی مورد ‌نظر با استفاده از طراحی پرایمر و تکنیک Polymerase chain reaction (PCR) تشدید ژنی شد و ژنوتیپ نمونه‌ها با روش PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) تعیین گردید.یافته‌ها: یافته‌ها حاکی از ارتباط آلل C از پلی‌مورفیسم M241T با ابتلا به سرطان‌ کولورکتال بود. همچنین، ارتباط معنی‌داری بین افزایش سن و سابقه‌ی فامیلی با بروز سرطان مشاهده ‌شد. از طرف دیگر، مصرف سیگار با سرطان ‌کولون رابطه‌ای را نشان نداد، اما نقش مؤثری در بروز و متاستاز سرطان ‌رکتوم داشت.نتیجه‌گیری: ارتباط پلی‌مورفیسم M241T با افزایش خطر بروز سرطان‌ کولورکتال در‌ کنار سابقه‌ی‌ فامیلی، سن بالا و مصرف سیگار، به عنوان عوامل خطر ابتلا به سرطان‌ کولورکتال گزارش گردید. از این‌رو، نتایج به دست آمده از پژوهش حاضر، ضرورت بررسی و انجام تحقیقات گسترده‌تر در مطالعات بعدی را مشخص نمود

کلیدواژه‌ها

عنوان مقاله [English]

The Association between Polymorphism of Xrcc3 Gene and Colorectal Cancer: A Case-Control Study

نویسندگان [English]

  • Majid Motovali-Bashi 1
  • Halimeh Rezaei 2
  • Jafar Maleki 3
  • Sheyda Khalilian 3
1 Associate Professor, Department of Biology, School of Science, University of Isfahan, Isfahan, Iran
2 PhD Student, Department of Biology, School of Science, University of Isfahan, Isfahan, Iran
3 MSc Student, Department of Biology, School of Science, University of Isfahan, Isfahan, Iran
چکیده [English]

Background: Colorectal cancer is the third cancer which results in death in western countries. Some of the risk factors of this cancer are age, inadequate diet, obesity, inactivity, genetic changes, etc. Considering the relationship between genetic changes with colorectal cancer is the subject of recent studies, and one of the subjects which have been focused is T241M polymorphism in Xrcc3 gene.Methods: In this case-control study, 90 patients with colorectal cancer and 83 healthy people were included. Genomic DNA was extracted from peripheral blood, and genotype distribution in the region of polymorphism was studied. By designing of primers, the considered area was amplified and genotyped using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) method.Findings: We observed the association between T241M polymorphism and colorectal cancer. Besides, there was a meaningful relationship between aging and family history with this cancer. On the other hand, smoking was not associated with colon cancer, but it also showed a significant role in the incidence and metastasis of rectal cancer.Conclusion: The relationship between T241M polymorphism and increased risk of colorectal cancer, along with family history of cancer, old age, and smoking were reported as risk factors for colorectal cancer. Therefore, the results of this research can show the need for further investigation in subsequent studies.

کلیدواژه‌ها [English]

  • Colorectal Cancer
  • Aging
  • Gender
  • Smoking

مراجع

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مجله دانشکده پزشکی اصفهان
دوره 36، شماره 475 - شماره پیاپی 475
خرداد و تیر 1397
صفحه 366-371

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سابقه مقاله

  • تاریخ دریافت: 23 فروردین 1397
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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