نوع مقاله : Review Article
نویسندگان
1 استادیار، مرکز تحقیقات عفونی اطفال، پژوهشکدهی بیماریهای واگیر، دانشگاه علوم پزشکی مازندران، ساری، ایران
2 استاد، مرکز تحقیقات عفونی اطفال، پژوهشکدهی بیماریهای واگیر، دانشگاه علوم پزشکی مازندران، ساری، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Background: The emergence of Extended-Spectrum Beta-Lactamase- and Carbapenemase- Producing Enterobacteriaceae is a threat to global health. Fast and accurate detection of these strains plays a key role in controlling nosocomial infections and effective treatment. In the present study, the detection methods of carbapenemase and broad-spectrum β-lactam-producing Enterobacteriaceae have been investigated, focusing on the summary of culture-based techniques and molecular methods.
Methods: The present study is a narrative review. The articles published between 2000 and 2022 were searched in international authoritative databases namely Scopus, PubMed, Scholar, Google, Web of Science, Science direct.
Findings: To identify Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae, conventional culture methods, biochemical methods, mass spectrometry, molecular methods, next generation sequencing, microarrays, whole genome sequencing, hybridization-based system, starch iodine assay, immunochromatography, colorimetry, mass spectrometry, electrochemical measurement, and flow cytometry were used.
Conclusion: For the detection of Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight), next-generation sequencing and whole genome sequencing are among the new and selected techniques.
کلیدواژهها [English]
https://clsi.org/standards/products/microbiology/documents/m100/