تأثیر تمرینات HIIT بر تغییرات Pannexin-1،P2X7، NLRP-1 بافت قلب موش‌های صحرایی نر نژاد ویستار مبتلا به دیابت نوع 2

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 کارشناس ارشد، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 استادیار، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 استاد، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران

4 استادیار، مرکز تحقیقات غدد و متابولیسم اصفهان، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقاله پژوهشی




مقدمه: هدف پژوهش حاضر، مطالعه‌ی تأثیر تمرینات HIIT بر تغییرات سطوح پروتئین‌های Pannexin-1، P2X7، NLRP-1 بافت قلب موش‌های صحرایی نر نژاد ویستار مبتلا به دیابت نوع 2 می‌باشد.
روش‌ها: در این مطالعه، تعداد 40 سر موش صحرایی نر 8 هفته‌ای از نژاد ویستار به طور تصادفی به چهار گروه سالم کنترل+سالم تمرین+دیابت تمرین+دیابت کنترل (10 = n) تقسیم شدند. به موش‌های گروه‌‌های مبتلا به دیابت به وسیله‌ی رژیم غذایی پرچرب و تزریق درون صفاقی استرپتوزوسین دیابت القا گردید. پس از آن برنامه‌ی تمرینات HIIT شامل 6 وهله 2 دقیقه‌ای تمرین با 80 درصد اوج سرعت در هفته اول بود که هر هفته 10 درصد به سرعت آن‌ها اضافه شد و تا 12 وهله در هفته‌ی هفتم و هشتم ادامه پیدا کرد. ۴8 ساعت پس از آخرین جلسه‌ی تمرین تحت شرایط استریل بافت قلب جدا گردید و با استفاده از روش وسترن‌بلات میزان سطوح پروتئین‌ مورد نظر اندازه‌گیری شد. از روش آماری one-way ANOVA و آزمون تعقیبی Tukey استفاده شد.
یافته‌ها: نتایج نشان داد، دیابت سبب افزایش معنی‌دار مقادیر پروتئین‌های Panexin-1، P2X7، NLRP-1 در بافت قلب موش‌های صحرایی مبتلا به دیابت نسبت به گروه سالم شد (0/5 ˂ P). همچنین مشخص شد، 8 هفته تمرین HIIT باعث کاهش معنی‌دار در مقادیر پروتئین‌های Panexin-1، P2X7، NLRP-1 در بافت قلب موش‌های صحرایی مبتلا به دیابت تمرین کرده نسبت به گروه دیابت کنترل شد (0/5 ˂ P).
نتیجه‌گیری: در نهایت می‌توان بیان کرد که تمرینات HIIT، نقش مفیدی در تنظیم سطح قند خون و مقادیر پروتئین‌های Pannexin-1، P2X7،NLRP-1  را در بافت قلب موش‌های صحرایی مبتلا به دیابت داشته و احتمالاً می‌تواند به عنوان یک راهبرد غیردارویی امیدوارکننده باشد.

تازه های تحقیق

علی اکبر علی زاده: Google Scholar, PubMed

امیرحسین احمدی رمضانی:  Google Scholar, PubMed

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of HIIT Exercises on the Changes of Pannexin-1, P2X7, and NLRP-1 in Heart Tissue of Male Wistar Rats with Type 2 Diabetes

نویسندگان [English]

  • Yasaman Rafiei 1
  • Aliakbar Alizadeh 2
  • Saeid Shakerian 3
  • Amirhossein Ramezani Ahmadi 4
1 MSc, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Assistant Professor, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Professor, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 Assistant Professor, Endocrine and Metabolism Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: The purpose of the present study is to study the effect of HIIT exercises on the changes in the levels of Pannexin-1, P2X7, and NLRP-1 proteins in the heart tissue of male Wistar rats with type 2 diabetes.
Methods: In this research, 40 8-week-old Wistar male rats were randomly divided into four groups: healthy control + healthy exercise + diabetes exercise + control diabetes (n = 10). Diabetic mice were induced by a high-fat diet and intraperitoneal injection of streptozocin. After that, the HIIT training program consisted of 6 2-minute sessions with 80% of the peak speed in the first week, which was increased by 10% every week and continued up to 12 sessions in the seventh and eighth weeks. 48 hours after the last training session, the heart tissue was isolated under sterile conditions, and the desired protein levels were measured using the western blot method. One-way statistical method and Tukey's post hoc test were used.
Findings: The results showed that diabetes caused a significant increase in the amount of Panexin-1, P2X7, and NLRP-1 proteins in the heart tissue of diabetic rats compared to the healthy group (P ˂ 0.5). It was also found that eight weeks of HIIT training caused a significant decrease in the amounts of Panexin-1, P2X7, and NLRP-1 proteins in the heart tissue of trained diabetic rats compared to the control diabetic group (P ˂ 0.5).
Conclusion: Finally, it can be stated that HIIT exercises have a beneficial role in regulating blood sugar levels and the amounts of Pannexin-1, P2X7, and NLRP-1 proteins in the heart tissue of diabetic rats and can probably be used as a Non-pharmacological strategy is promising.

کلیدواژه‌ها [English]

  • Diabetic cardiomyopathy
  • HIIT
  • Pannexin-1
  • P2X7
  • NLRP-1

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مجله دانشکده پزشکی اصفهان
دوره 42، شماره 769
هفته 1، مرداد
مرداد و شهریور 1403
صفحه 439-449

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  • تاریخ دریافت: 03 تیر 1403
  • تاریخ پذیرش: 27 مرداد 1403

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