The Effect of Tissue-Engineered Wound Dressing Comprising Copper, on the Healing Process of Full-Thickness Wound in Mouse Model

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Tissue Engineering, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran

2 Assistant Professor, Department of Emergency Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

3 General Practitioner, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

4 Assistant Professor, Department of Anatomical Sciences, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran

5 Assistant Professor, Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

Background: Production of skin dressings or substitutes is one of the most important tissue engineering fields. Since copper is an important agent in skin extracellular matrix synthesis, we investigated the effect of collagen-gelatin-bioglass scaffolds containing copper in accelerating the healing process of full-thickness skin wound in mouse model.Methods: We used 12 mice with two identical skin wounds on their back (with 5 mm diameter, circular, and full-thickness), one wound as control, second dressed with collagen-gelatin-bioglass scaffold, and third dressed with collagen-gelatin-bioglass scaffold containing copper. After 14 days, the wound healing process was analyzed using both macroscopic and microscopic (after hematoxylin and eosin staining) methods.Findings: The wound dressings had porous structure, and were biocompatible. They improved the healing process of full-thickness wound in mouse model. This process was statistically much better in the dressings comprising copper (P < 0.05).Conclusion: Since wounds dressed with collagen-gelatin-bioglass scaffold containing copper improved wound healing in animal models, it can be suggested as a new approach in design and synthesis of wound dressings for human.

Keywords

References

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Journal of Isfahan Medical School
Volume 36, Issue 478 - Serial Number 478
March and April 2018
Pages 472-477

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History

  • Receive Date: 28 February 2018
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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