The Effect of Six Weeks of High-Intensity Interval Training with Hawthorn Extract Supplementation on the Changes of BAX/Bcl-2 Indices in the Hippocampus Tissue of Aged Male Wistar Rats

Document Type : Original Article(s)

Authors

1 MSc, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Assistant Professor, Department of Sport Physiology, School of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Assistant Professor, Health Through Exercise and Active Living (HEAL) Research Unit, School of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu 88400, Sabah, Malaysia and Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Malaysia Sabah, Jalan UMS, Kota Kinabalu 88450, Sabah, Malaysia

Abstract

Background: Aging is a complex and multifactorial phenomenon characterized by a gradual decrease in physiological functions in various organs. The central nervous system and the hippocampus are significantly affected by the effects of aging, such as apoptosis. The synergistic effects of physical activity and natural antioxidants such as hawthorn to protect brain damage against apoptosis may be the key to inhibiting or reducing the apoptosis of nerve cells in the aging process. Therefore, this study aims to investigate the effect of high-intensity interval training with hawthorn extract supplementation on the changes of BAX/Bcl-2 indices in the hippocampus tissue of aged rats.
Methods: In this experimental research, 30 male Wistar field rats 22 months of age were randomly divided into five groups of six, including young, elderly, elderly/exercise, elderly/extract, and elderly/extract/exercise. In the groups that used hawthorn extract, they received 100 mg/kg of the extract by gavage after training sessions. The high-intensity interval training protocol was for six weeks with an intensity of 80-100% of maximum speed. After tissue removal, BAX/Bcl-2 protein levels were measured using the western blot method. One-way ANOVA and Tukey's post hoc test were used to examine the research variables.
Findings: The results of the present study showed that aging caused a significant decrease in the Bcl-2 variable and a significant increase in the BAX variable (P = 0.001). Also, the results of this research showed that both high-intensity interval training and hawthorn extract consumption caused a consequential advance in Bcl-2 values (respectively, P = 0.031 and P = 0.011) and a significant decrease in BAX protein (P = 0.003 and P = 0.016, respectively). In addition, the results of this research showed that the synergistic effects of high-intensity interval training in the elderly/extract/exercise group caused a significant increase and decrease in Bcl-2 and BAX variables, respectively, compared to the elderly/exercise and elderly/extract groups (P < 0.05).
Conclusion: According to the results of this research, it can be stated that the synergy of the beneficial effects of high-intensity intermittent physical activity along with the consumption of hawthorn extract in increasing the Bcl-2 variable and decreasing the BAX variable can probably play a consequential role in inhibiting the apoptosis process in the rat hippocampus tissue and can probably be a promising non-pharmacological strategy.

Highlights

Fatemeh Kianizadegan:  Google Scholar

Mohammad Rami: Google Scholar, PubMed

Aliakbar Alizadeh: Google Scholar, PubMed

Maryama Awang Daud: Google Scholar, PubMed

Keywords

Main Subjects

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Journal of Isfahan Medical School
Volume 42, Issue 769
July and August 2024
Pages 451-462

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History

  • Receive Date: 25 June 2024
  • Accept Date: 11 August 2024

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