ارزیابی سمیت نانو میله‏ های اکسید آهن بر رده‌ی سلولی 929L

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه فیزیولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران

2 استاد، انستیتو پاستور ایران، بانک سلولی ایران، تهران، ایران

3 استادیار، گروه انرژی‌های نو و محیط زیست، دانشکده‌ی فناوری‌های نوین، دانشگاه تهران، تهران، ایران

4 دانشیار، گروه فیزیولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران

چکیده

مقدمه: کاربردهای عمده‏ی نانو فن‌آوری در صنعت، کشاورزی، بیولوژی و پزشکی رو به افزایش است. با توجه به گستره‏ی وسیع نانو ساختار‌ها در علوم پزشکی، این پژوهش با هدف ارزیابی سمیت سلولی نانو ذرات اکسید آهن از طریق مقایسه‌ی میزان زیست پذیری و آپوپتوز سلولی، انجام شد.روش‌ها: در این مطالعه، نانو میله‌ها به روش هم‌رسوبی ساخته شدند. برای تعیین اندازه و شکل نانو ساختار‏ها، از میکروسکوپ الکترونی عبوری و میکروسکوپ الکترونی روبشی استفاده شد. دوزهای μg/ml 200 و 800 نانو میله‌ها با پوشش اوره و پلی اتیلن گلیکول و به شکل اصلاح شده و نشده، در 48 و 72 ساعت از طریق آزمایش MTT مورد ارزیابی سمیت قرار گرفتند.یافته‌ها: نانو میله‏های اکسید آهن با پوشش اوره، به شکل میله‏ای با اندازه‏ی طولی nm 150 و اندازه‏ی قطر nm 15 و نانو میله‏های اکسید آهن با پوشش PEG (Polyethylene glycol) دارای طول nm 150 و قطر nm 23 بودند. زیست پذیری سلول‌های قرار گرفته در معرض نانو میله‎های اکسید آهن اصلاح نشده، نسبت به نوع اصلاح شده‎ی آن کمتر بود. این سمیت، با افزایش دوز روند صعودی نشان داد. زیست پذیری سلول‌های قرار گرفته در معرض نانو میله‎های اکسید آهن با پوشش PEG کمتر از نانو میله‌های دارای پوشش اوره بودند.نتیجه‌گیری: افزایش مرگ سلولی توسط نانو میله‏های اصلاح نشده، می‏تواند ناشی از تشکیل حلقه‏ی پروتئینی به دور این نانو میله‏ها در محیط حاوی پروتئین باشد. علاوه بر این، افزایش مرگ سلولی توسط نانو میله‏های دارای پوشش PEG در مقایسه با اوره، بیانگر تأثیر نوع پوشش و نوع سلول مورد مطالعه بر سمیت سلولی آن‌ها است.

کلیدواژه‌ها

عنوان مقاله [English]

The Iron Oxide Nanorods Toxicity on L929 Cell Line

نویسندگان [English]

  • Sarieh Ghasempour 1
  • Mohammad-Ali Shokrgozar 2
  • Roghayeh Ghasempour 3
  • Mohsen Alipour 4
1 MSc Student, Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2 Professor, Pasteur Institute of Iran, National Cell Bank, Tehran, Iran
3 Assistant Professor, Department of Renewable Energy of Environment Engineering, School of New Science of Technology, University of Tehran, Tehran, Iran
4 Associate Professor, Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
چکیده [English]

Background: Major applications of nanotechnology in industry, agriculture, biology and medicine are growing. Given the broad range of nanoscience in medical sciences, evaluation of the cytotoxicity of iron oxide nanorods through comparing viability and apoptosis formed the objectives of this study.Methods: In this study, the nanorods were synthesized by coprecipitation method and transmission electron microscopy and scanning electron microscopy was used for determination of the size and shape of nanoparticles. 200 and 800 μg/ml urea and polyethylene glycol (PEG) coated nanorods, in forms of modified and non-modified, were assessed for toxicity using MTT assay 48 and 72 hours later.Findings: The length and diameter of the urea- and PEG-coated nanorods were 150 and 15 nm and 150 and 23 nm, respectively. Viability of cells exposed to non-modified iron oxide nanorods was less than modified form. This toxicity showed uptrend with increasing dose. Viability of the cells exposed to PEG-coated iron oxide nanorods was lower than urea-coated once.Conclusion: It appears that the increase in apoptosis affected by non-modified iron oxide nanorods might be resulted from formation of protein rings called Hard Corona around the nanorods. In addition, more increase of cell death by PEG-coated nanorods compared to urea-coated nanorods is indicator of the effect of type of coverage and type of cells on their cytotoxicity.

کلیدواژه‌ها [English]

  • Iron oxide nanorods
  • MTT
  • Viability
  • Urea coated iron oxide
  • L929 cell line

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مجله دانشکده پزشکی اصفهان
دوره 31، شماره 263 - شماره پیاپی 263
بهمن و اسفند 1392
صفحه 1973-1984

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سابقه مقاله

  • تاریخ دریافت: 04 تیر 1392
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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