Induction of Neuron-Like Cells from Adipose Derived Stem Cells in Alginate Hydrogel, Using Neurospheres Formation

Document Type : Original Article (s)

Authors

1 Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 PhD Student, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

3 Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 PhD Student, Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Hydrogels provide appropriate three-dimensional environment for culture of a variety of cells and cell encapsulation in hydrogels is a promise plan for tissue engineering applications. Alginate is an attractive biocompatible hydrogel that provides a supportive system for the encapsulated cells. Moreover, human adipose derived stem cells are mesenchymal stem cells that might be a suitable source of cells for use in autologous cell therapy.Methods: In this study, we examined the fate of human adipose derived stem cells encapsulated in alginate hydrogel that cultured in neural induction medium for 1 week. Using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and immunocytochemical analysis, the proliferation rate, and viability and neural differentiation of human adipose derived stem cells were evaluated.Findings: We observed a significant increase in the mean percent of Nestin, glial fibrillary acidic protein (GFAP) and microtubule-associated protein-2 (MAP2) positive cells and significant reduction of proliferation rate and viability in encapsulated cells versus monolayer induced cells.Conclusion: These findings showed that alginate hydrogel can provide a suitable environment for neural differentiation of human adipose derived stem cells.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 288 - Serial Number 288
May and June 2014
Pages 828-840

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History

  • Receive Date: 01 February 2014
  • Revise Date:
  • Accept Date: 06 April 2022

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