Improvement of Sperm Evaluation Parameters Following Ghrelin Treatment in Cadmium-Induced Testicular Injury in Rats

Document Type : Original Article (s)

Author

Associate Professor, Department of Clinical Sciences, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran

Abstract

Background: Cadmium, as an environmental and industrial pollutant, due to its in-vivo accumulation in tissues and especially in the testis results in extensive degenerative changes. The aim of this study was to determine the possible protective effects of ghrelin against cadmium and on sperm quality parameters in rats.Methods: Thirty adult male rats were allocated into three equal groups, control-saline, cadmium-saline and cadmium-ghrelin. Induction of testicular injury was achieved by a single injection of 2 mg/kg cadmium chloride intraperotoneally; 10 nmol of ghrelin was given 1 day before cadmium injection and continued for 10 consecutive days. Five rats from each group were sacrificed on the days 5 and 10 after cadmium toxicity and sperm was taken from the epididymal tail for the evaluation.Findings: Testicular weight and sperm concentration significantly decreased by cadmium toxicity (P < 0.05). Likewise, the percentages of sperm progressive motility on the day 10 and membrane integrity on both days significantly decreased in cadmium group (P < 0.05 for all). Whereas, ghrelin treatment enhanced hypo-osmotic swelling test (positive cells) on the days 5 and 10 compared to the cadmium-saline group (P < 0.05). However, it failed to increase progressive motility, sperm concentration or testicular weight significantly.Conclusion: Ghrelin, due to its possible antioxidant properties, caused an increase in sperm membrane integrity that can be prerequisite for progressive movement. Ghrelin could be implicated for attenuation of cadmium effects on sperm quality.

Keywords

References

  1. Thompson J, Bannigan J. Cadmium: toxic effects on the reproductive system and the embryo. Reprod Toxicol 2008; 25(3): 304-15.
  2. Siu ER, Mruk DD, Porto CS, Cheng CY. Cadmium-induced testicular injury. Toxicol Appl Pharmacol 2009; 238(3): 240-9.
  3. Oldereid NB, Thomassen Y, Attramadal A, Olaisen B, Purvis K. Concentrations of lead, cadmium and zinc in the tissues of reproductive organs of men. J Reprod Fertil 1993; 99(2): 421-5.
  4. Ognjanovic BI, Markovic SD, Ethordevic NZ, Trbojevic IS, Stajn AS, Saicic ZS. Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: protective role of coenzyme Q(10) and vitamin E. Reprod Toxicol 2010; 29(2): 191-7.
  5. Sen GR, Sen GE, Dhakal BK, Thakur AR, Ahnn J. Vitamin C and vitamin E protect the rat testes from cadmium-induced reactive oxygen species. Mol Cells 2004; 17(1): 132-9.
  6. Stohs SJ, Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med 1995; 18(2): 321-36.
  7. Koyuturk M, Yanardag R, Bolkent S, Tunali S. Influence of combined antioxidants against cadmium induced testicular damage. Environ Toxicol Pharmacol 2006; 21(3): 235-40.
  8. Yiin SJ, Chern CL, Sheu JY, Lin TH. Cadmium induced lipid peroxidation in rat testes and protection by selenium. Biometals 1999; 12(4): 353-9.
  9. Kojima M, Kangawa K. Ghrelin: structure and function. Physiol Rev 2005; 85(2): 495-522.
  10. van der Lely AJ, Tschop M, Heiman ML, Ghigo E. Biological, physiological, pathophysiological, and pharmacological aspects of ghrelin. Endocr Rev 2004; 25(3): 426-57.
  11. Tena-Sempere M. Ghrelin: novel regulator of gonadal function. J Endocrinol Invest 2005; 28(5 Suppl): 26-9.
  12. Barreiro ML, Gaytan F, Castellano JM, Suominen JS, Roa J, Gaytan M, et al. Ghrelin inhibits the proliferative activity of immature Leydig cells in vivo and regulates stem cell factor messenger ribonucleic acid expression in rat testis. Endocrinology 2004; 145(11): 4825-34.
  13. Kheradmand A, Alirezaei M, Asadian P, Rafiei AE, Joorabi S. Antioxidant enzyme activity and MDA level in the rat testis following chronic administration of ghrelin. Andrologia 2009; 41(6): 335-40.
  14. Kheradmand A, Alirezaei M, Birjandi M. Ghrelin promotes antioxidant enzyme activity and reduces lipid peroxidation in the rat ovary. Regul Pept 2010; 162(1-3): 84-9.
  15. Ikemoto I, Machida T, Tanaka A, Kotera S, Mikuriya H, Shirai T. Appearance of ldh x activity in rat serum after acute and subacute testicular damages. Japanese Journal Of Fertility & Sterility 1990; 35(2): 247-52.
  16. Yang HS, Han DK, Kim JR, Sim JC. Effects of alpha-tocopherol on cadmium-induced toxicity in rat testis and spermatogenesis. J Korean Med Sci 2006; 21(3): 445-51.
  17. Agarwal A, Ikemoto I, Loughlin KR. Prevention of testicular damage by free-radical scavengers. Urology 1997; 50(5): 759-63.
  18. Taati M, Moghadasi M, Dezfoulian O, Asadian P, Kheradmand A, Abbasi M, et al. The effect of ghrelin pretreatment on epididymal sperm quality and tissue antioxidant enzyme activities after testicular ischemia/reperfusion in rats. J Physiol Biochem 2012; 68(1): 91-7.
  19. Cancel AM, Lobdell D, Mendola P, Perreault SD. Objective evaluation of hyperactivated motility in rat spermatozoa using computer-assisted sperm analysis. Hum Reprod 2000; 15(6): 1322-8.
  20. Sonmez M, Turk G, Yuce A. The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenology 2005; 63(7): 2063-72.
  21. Sliwa L, Macura B. Evaluation of cell membrane integrity of spermatozoa by hypoosmotic swelling test - "water test" in mice after intraperitoneal daidzein administration. Arch Androl 2005; 51(6): 443-8.
  22. Aoki A, Hoffer AP. Reexamination of the lesions in rat testis caused by cadmium. Biol Reprod 1978; 18(4): 579-91.
  23. Kara H, Cevik A, Konar V, Dayangac A, Yilmaz M. Protective effects of antioxidants against cadmium-induced oxidative damage in rat testes. Biol Trace Elem Res 2007; 120(1-3): 205-11.
  24. Amara S, Abdelmelek H, Garrel C, Guiraud P, Douki T, Ravanat JL, et al. Preventive effect of zinc against cadmium-induced oxidative stress in the rat testis. J Reprod Dev 2008; 54(2): 129-34.
  25. Bauer R, Demeter I, Hasemann V, Johansen JT. Structural properties of the zinc site in Cu,Zn-superoxide dismutase; perturbed angular correlation of gamma ray spectroscopy on the Cu, 111Cd-superoxide dismutase derivative. Biochem Biophys Res Commun 1980; 94(4): 1296-302.
  26. Fernandez-Fernandez R, Navarro VM, Barreiro ML, Vigo EM, Tovar S, Sirotkin AV, et al. Effects of chronic hyperghrelinemia on puberty onset and pregnancy outcome in the rat. Endocrinology 2005; 146(7): 3018-25.
  27. Martini AC, Fernandez-Fernandez R, Tovar S, Navarro VM, Vigo E, Vazquez MJ, et al. Comparative analysis of the effects of ghrelin and unacylated ghrelin on luteinizing hormone secretion in male rats. Endocrinology 2006; 147(5): 2374-82.
  28. Barreiro ML, Suominen JS, Gaytan F, Pinilla L, Chopin LK, Casanueva FF, et al. Developmental, stage-specific, and hormonally regulated expression of growth hormone secretagogue receptor messenger RNA in rat testis. Biol Reprod 2003; 68(5): 1631-40.
  29. Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 1984; 70(1): 219-28.
  30. Dobranic T, Samardžija M, Cergolj M, Prvanovic N. Determination of membrane integrity of canine spermatozoa. Vet Arhiv 2005; 75(1): 23-30.
  31. Zavos PM. Hypoosmotic swelling test (HOS)/ functional integrity of sperm membrane. J Assisst Reprod Technol Androl 1990; 2: 215-6.
  32. Chan SY, Fox EJ, Chan MM, Tsoi WL, Wang C, Tang LC, et al. The relationship between the human sperm hypoosmotic swelling test, routine semen analysis, and the human sperm zona-free hamster ovum penetration assay. Fertil Steril 1985; 44(5): 668-72.
  33. Dong MH, Kaunitz JD. Gastroduodenal mucosal defense. Curr Opin Gastroenterol 2006; 22(6): 599-606.
  34. Aurich JE, Schonherr U, Hoppe H, Aurich C. Effects of antioxidants on motility and membrane integrity of chilled-stored stallion semen. Theriogenology 1997; 48(2): 185-92.
  35. Ball BA, Medina V, Gravance CG, Baumbe J. Effect of antioxidants on preservation of motility,viability and acrosomal integrity of equine spermatozoa during storage at 5 degrees C. Theriogenology 2001; 56(4): 577-89.
  36. Zwirska-Korczala K, Adamczyk-Sowa M, Sowa P, Pilc K, Suchanek R, Pierzchala K, et al. Role of leptin, ghrelin, angiotensin II and orexins in 3T3 L1 preadipocyte cells proliferation and oxidative metabolism. J Physiol Pharmacol 2007; 58(Suppl 1): 53-64.
  37. Peltola V, Huhtaniemi I, Ahotupa M. Antioxidant enzyme activity in the maturing rat testis. J Androl 1992; 13(5): 450-5.
  38. Tramer F, Rocco F, Micali F, Sandri G, Panfili E. Antioxidant systems in rat epididymal spermatozoa. Biol Reprod 1998; 59(4): 753-8.
Journal of Isfahan Medical School
Volume 31, Issue 265 - Serial Number 265
November and December 2014
Pages 2053-2062

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History

  • Receive Date: 28 May 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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