فعال‌سازی مصنوعی تخمک: انواع روش‌ها و تأثیر کلینیکی آن پس از تزریق درون سیتوپلاسمی اسپرم

نوع مقاله : مقاله مروری

نویسندگان

1 کارشناس ارشد، پژوهشگاه رویان، پژوهشکده‌ی زیست فناوری جهاد دانشگاهی، مرکز تحقیقات پزشکی تولید مثل، گروه زیست فناوری تولید مثل و مرکز باروری و ناباروری اصفهان، اصفهان، ایران

2 دانشجوی دکتری و عضو هیأت علمی، پژوهشگاه رویان، پژوهشکده‌ی زیست فناوری جهاد دانشگاهی، مرکز تحقیقات پزشکی تولید مثل، گروه زیست فناوری تولید مثل، اصفهان، ایران

3 دانشیار، گروه تولید مثل و تکوین، پژوهشگاه رویان، پژوهشکده‌ی زیست فناوری جهاد دانشگاهی، مرکز تحقیقات پزشکی تولید مثل، گروه زیست فناوری تولید مثل و مرکز باروری و ناباروری اصفهان، اصفهان، ایران

چکیده

مقدمه: عدم دستیابی به لقاح یک پدیده‌ی نادر پس از تزریق اسپرم به درون سیتوپلاسم تخمک (Intracytoplasmic sperm injection یا ICSI) است. یکی از دلایل اصلی آن، فعال نشدن تخمک توسط اسپرم می‌باشد. جهت غلبه بر عدم دستیابی به لقاح، از روش‌های مصنوعی جهت فعال‌سازی تخمک استفاده می‌گردد. هدف از ارائه‌ی این مقاله‌ی مروری، آشنایی مختصر با مکانیسم فعال شدن تخمک توسط اسپرم است و سپس شرحی بر روش‌های استفاده‌شده جهت فعال‌سازی مصنوعی تخمک و نگرانی‌های مرتبط با آن، بود. همچنین در این مطالعه‌ی مروری، به بررسی اثرات استفاده از روش‌های فعال‌سازی مصنوعی تخمک بر روی بیماران مختلف و نتایج کلینیکی آن پرداخته شد.روش‌ها: در این مطالعه مقالات جستجو‌شده در پایگاه‌های اطلاعاتی PubMed و پایگاه‌های مرتبط با مقالات ISI از سال‌های 1930 تا 2012 میلادی بررسی شدند.یافته‌ها: استفاده از روش‌های فعال‌سازی مصنوعی تخمک می‌تواند باعث افزایش میزان لقاح و دستیابی به حاملگی در برخی بیماران گردد. به طور عمده روش‌های مکانیکی، الکتریکی و شیمیایی جهت فعال‌سازی مصنوعی تخمک پس از عمل ICSI مورد استفاده قرار می‌گیرند که روش فعال‌سازی شیمیایی کاربرد گسترده‌تری دارد.نتیجه‌گیری: مطالعات انجام‌شده در این زمینه نشان می‌دهد که استفاده از روش‌های فعال‌سازی مصنوعی تخمک در برخی از بیمارانی که کاندیدای استفاده از این روش‌ها می‌باشند، نتیجه بهتری داشته است و باعث افزایش میزان لقاح و دستیابی به حاملگی شده است. اگر چه روش‌هایی جهت پیشگویی و بررسی پتانسیل لقاح نمونه‌ی سمن افراد مورد توجه و تحقیق قرار گرفته است، ولی هنوز مطالعات گسترده‌ای برای تثبیت یک روش ایمن بدین منظور نیاز است.

کلیدواژه‌ها

عنوان مقاله [English]

Artificial Oocyte Activation: Methods and Clinical Efficiency after Intra-Cytoplasmic Sperm Injection

نویسندگان [English]

  • Mohammad Reza Deemeh 1
  • Marzieh Tavalaee 2
  • Mohammad Hossein Nasr-Esfahani 3
1 Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, Academic Center for Education, Culture and Researc (ACECR) AND Isfahan Fertility and Infertility Center, Isfahan, Iran
2 PhD Student AND Faculty Member, Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, Academic Center for Education, Culture and Researc (ACECR), Isfahan, Iran
3 Associate Professor, Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, Academic Center for Education, Culture and Researc (ACECR) AND Isfahan Fertility and Infertility Center, Isfahan, Iran
چکیده [English]

Background: Failed fertilization is a rare event occurring after intra-cytoplasmic sperm injection (ICSI). Failure of oocyte activation considered as main reason for failed fertilization. For overcome of failed fertilization, Artificial Oocyte Activation (AOA) techniques after ICSI are applied and improved. This review article is a brief introduction to oocyte activation, followed by description of the methods for AOA and related concern regarding AOA. Also, the clinical effect of using AOA in different type of infertile individual was considered.Methods: Literature search in Entrez PubMed as well as other data sources from1930 to 2012 was done.Findings: Mechanical, electrical and chemical methods mainly used for AOA and chemical activation methods as the most commonly were performed. Also, using AOA after ICSI, fertilization rate was increased and possibly achieved pregnancy in some infertile individuals.Conclusion: AOA in some type of infertile individual increased fertilization rate and achieving pregnancy. Although, some techniques may be useful for predict and assessment of semen fertilization potential, but further studies are required for establishing method of this purpose.

کلیدواژه‌ها [English]

  • Intra-cytoplasmic sperm injection (ICSI)
  • Failed fertilization
  • Oocyte activation

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مجله دانشکده پزشکی اصفهان
دوره 31، شماره 240 - شماره پیاپی 240
مرداد و شهریور 1392
صفحه 851-866

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  • تاریخ دریافت: 28 آبان 1391
  • تاریخ بازنگری: 11 آبان 1403
  • تاریخ پذیرش: 17 فروردین 1401

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