In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions

Document Type : Original Article (s)

Authors

1 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

2 Student of Medicine, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

3 Professor, Department of Nephrology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Department of Statistics, School of Health, Shahrekord University of Medical Sciences, Shahrekord, Iran

5 Professor, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

Background: Ampeloprasum is traditionally used for gastrointestinal disorders. In this experimental study, the effect of ethanolic extract of Allium ampeloprasum was investigated on the rat ileum contractions. The mechanisms were also evaluated.Methods: In this experimental study, 48 male Wistar rats were randomly designated into six equal groups, including control and cumulative concentrations of Allium ampeloprasum, propranolol, naloxone, L-name, and CaCl2. In the day of the experiment, the ileum was dissected and its contractions were recorded in Tyrode solution in an organ bath. To evaluate the mechanisms involved, propranolol, naloxone, or L-name was incubated and the percent of changes in contractions were recorded.Findings: The extract of Allium ampeloprasum dose-dependently reduced the contractions induced by KCl (60 mM) (P < 0.0001); propranolol reversed this effect (P < 0.0010), but L-name and naloxone were not effective. CaCl2 increased the contractions (P < 0.0010) and the extract reversed this effect.Conclusion: Allium ampeloprasum leaves can affect the ileum contractions through beta adrenoreceptors and voltage-dependent calcium channels and might be used in gastrointestinal disorders.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 268 - Serial Number 268
November and December 2014
Pages 2238-2249

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History

  • Receive Date: 11 July 2013
  • Revise Date: 02 November 2024
  • Accept Date: 06 April 2022

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