Frequency of Bk Virus in Urine Samples of Childrens Below 18 Years Old with Acute Lymphoblastic Leukemia in Comparision to Normal Populatin

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Pediatrics, Child Health Promotion Research Center, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

2 Associate Professor, Department of Pediatrics, Nephrology Research Center, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

3 Intern, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

4 Assistant Professor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

5 Department of Epidemiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Abstract

Background: BK virus belongs to the polyomaviridae family. It has been reported that up to 80% of normal population change to seropositive status between 5 and 10 years of age. It has been assumed that the rate of multiple virus isolation is significantly greater in children with acute lymphoblastic leukemia (ALL) than normal population. Considering the scarce data on the presence of urinary excretion of BKV in children with ALL, we studied the urine BKV in newly diagnosed leukemic children comparing with normal population.Methods: 62 subjects (31 all cases and 31 controls) were enrolled in the study. Epitelial cells isolated from urine samples were screened for the BKV DNA with PCR method.Finding: Positive PCR for urine BK virus was seen in 3 children (9.7%). No positive result for urine BKV was achieved in control group. However, Fisher Exact test did not show any significant difference between two groups (P > 0.05). In addition, there was no significant correlation between BKV positivity and frequency of relapses. Furthermore, the mean time of diagnosis was not different between BKV positive and negative groups.Conclusion: In our study there was not any significant difference between BK viruria in ALL children and control group, no case of BK viruria had been found in control group. To demonstrate the role of BKV in inducing ALL or increasing number of relapses, prospective studies on larger scale of population and evaluating both serum and urine for BKV are recommended. 

Keywords

References

  1. Inaba H, Jones DP, Gaber LW, Shenep JL, Call SK, Pui CH, et al. BK virus-induced tubulointerstitial nephritis in a child with acute lymphoblastic leukemia. J Pediatr 2007; 151(2): 215-7.
  2. Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, et al. Fields Virology. 4th ed. Philadelphia: Lippincott Williams & Wilkins; 2001.
  3. Kunitake T, Kitamura T, Guo J, Taguchi F, Kawabe K, Yogo Y. Parent-to-child transmission is relatively common in the spread of the human polyomavirus JC virus. J Clin Microbiol 1995; 33(6): 1448-51.
  4. Reploeg MD, Storch GA, Clifford DB. Bk virus: a clinical review. Clin Infect Dis 2001; 33(2): 191-202.
  5. Negrini M, Castagnoli A, Pavan JV, Sabbioni S, Araujo D, Corallini A, et al. Suppression of tumorigenicity and anchorage-independent growth of BK virus-transformed mouse cells by human chromosome 11. Cancer Res 1992; 52(5): 1297-303.
  6. Feldman A. Acute lymphoblastic leukemia. Pediatric Oncology 2006; 10: 467-9.
  7. George SL, Aur RJ, Mauer AM, Simone JV. A reappraisal of the results of stopping therapy in childhood leukemia. N Engl J Med 1979; 300(6): 269-73.
  8. Greaves MF. Speculations on the cause of childhood acute lymphoblastic leukemia. Leukemia 1988; 2(2): 120-5.
  9. Alexander FE, Chan LC, Lam TH, Yuen P, Leung NK, Ha SY, et al. Clustering of childhood leukaemia in Hong Kong: association with the childhood peak and common acute lymphoblastic leukaemia and with population mixing. Br J Cancer 1997; 75(3): 457-63.
  10. Ross JA, Spector LG. Cancers in children. In: Schottenfeld D, Fraumeni J, editors. Cancer Epidemiology and Prevention. 3 ed. New York: Oxford University Press; 2006. p. 1251-68.
  11. Vanchiere JA, White ZS, Butel JS. Detection of BK virus and simian virus 40 in the urine of healthy children. J Med Virol 2005; 75(3): 447-54.
  12. Tognon M, Corallini A, Martini F, Negrini M, Barbanti-Brodano G. Oncogenic transformation by BK virus and association with human tumors. Oncogene 2003; 22(33): 5192-200.
  13. Hernandez-Losa J, Fedele CG, Pozo F, Tenorio A, Fernandez V, Castellvi J, et al. Lack of association of polyomavirus and herpesvirus types 6 and 7 in human lymphomas. Cancer 2005; 103(2): 293-8.
  14. Ma X, Urayama K, Chang J, Wiemels JL, Buffler PA. Infection and pediatric acute lymphoblastic leukemia. Blood Cells Mol Dis 2009; 42(2): 117-20.
  15. McNally RJ, Eden TO, Alexander FE, Kelsey AM, Birch JM. Is there a common aetiology for certain childhood malignancies? Results of cross-space-time clustering analyses. Eur J Cancer 2005; 41(18): 2911-6.
  16. Smith MA, Strickler HD, Granovsky M, Reaman G, Linet M, Daniel R, et al. Investigation of leukemia cells from children with common acute lymphoblastic leukemia for genomic sequences of the primate polyomaviruses JC virus, BK virus, and simian virus 40. Med Pediatr Oncol 1999; 33(5): 441-3.
Journal of Isfahan Medical School
Volume 29, Issue 141 - Serial Number 141
May and June 2011
Pages 665-671

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History

  • Receive Date: 18 May 2011
  • Revise Date: 24 May 2024
  • Accept Date: 06 April 2022

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