Document Type : Original Article (s)
Authors
1
Associate Professor, Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
2
Department of Microbiology, Jahrom Branch, Young Researcher's Club, Islamic Azad University, Jahrom, Iran
3
Associate Professor, Department of Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4
Assistant Professor, Department of Neurology, School of Medicine, Hormozgan University of Medical Sciences, Hormozgan, Iran
5
Department of Microbiology, Young Researcher's Club, Jahrom Branch, Islamic Azad University, Jahrom, Iran
Abstract
Background: Microsatellites are the short tandem repeat regions in eukaryotic genome whose polymorphisms have an association with some population genetic properties and also some diseases. In this study, we aimed to investigate the gene polymorphism of tumor necrosis factor alpha (TNF-α) of human leukocyte antigen (HLA) locus in native population of Southern Iran. Methods: This cross-sectional descriptive study was performed on 50 peripheral blood samples of native Southern Iranians. Allelic polymorphisms of TNF-α were studied using specialized primers. Statistical analyses were computed by SPSS and Popgene software programs. Findings: Polymorphism evaluations showed that all 13 typical alleles of TNF-α were present in this Iranian group. TNF-α*3 and TNF-α*4 were the most prevalent alleles with frequencies of 0.24 and .016, respectively. On the other hand, TNF-α*1 and TNF-α*2 were the rarest alleles with frequency of 0.02. In addition, the differences between the frequencies of alleles and the studied population were significant (P < 0.05). Conclusion: HLA microsatellites can be useful in identifying the relation between gene polymorphism of TNF and genetic diseases in different parts of Iran. Then can also be beneficial in preparing the first Iranian STR gene database. Keywords: Gene Polymorphism, Microsatellite, Tumor Necrosis Factor Alpha
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