Evaluation of Leishmanicidal Activity of Oxypeucedanin and Isoimperatorin on Leishmania major Promastigotes

Document Type : Original Article (s)

Authors

1 Professor, Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Assistant Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Lecturer, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Assistant Professor, Department of Biostatistics, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran

5 Student of Pharmacy, Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences AND Isfahan Pharmaceutical Sciences Research Center AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Leishmaniasis has a wide range of clinical symptoms including cutaneous, mucocutaneous, and visceral leishmaniasis. Recently, advances have been observed in the field of using herbal medicines for the treatment of leishmaniasis. The study aimed to determine the in-vitro effect of two prenylated coumarins (oxypeucedanin and isoimperatorin) of Prangos ferulaceae (L.) Lindl with positive and negative controls on Leishmania major.Methods: To have stationary phase of the growth curve, the parasites were cultured in NNN (Novy-MacNeal-Nicolle) and RPMI-1640 (Roswell Park Memorial Institute 1640) medias in the incubator. Then a certain amount of culture, containing approximately one million parasites, were exposed to oxypeucedanin and isoimperatorin and their effects were investigated after 3, 6, 24, 48, and 72 hours.Findings: Non of the concentrations of oxypeucedanin and isoimperatorin had leishmanicidal effect compared to the positive control (P < 0.05).Conclusion: Oxypeucedanin and isoimperatorin do not have leishmanicidal effect on Leishmania major promastigotes.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 235 - Serial Number 235
March and April 2013
Pages 581-590

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History

  • Receive Date: 04 March 2013
  • Revise Date: 23 May 2024
  • Accept Date: 06 April 2022

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