Study of Cell Behavior of the Electrospun Polycaprolactone/Gelatin Scaffold Containing Nano-hydroxyapatite and Vitamin D3

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Biomedical Engineering, School of Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Associate Professor, Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Iran Polymer and Petrochemical Institute AND Department of Biomedical Engineering, School of Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

4 Assistant Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Scaffold, an essential element of tissue engineering, should provide proper physical and chemical properties and evolve suitable cell behavior for tissue regeneration.Methods: Polycaprolactone/gelatin-based nanocomposite scaffolds containing nano-hydroxyapatite and vitamin D3 was fabricated using the electrospinning method. To obtain suitable properties, solution and process parameters were optimized. Fiber morphology and MG-63 cells were cultured; chemical interactions between molecules forming scaffold and the amount of mineral deposition were determined via scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR) and alizarin red staining, respectively.Findings: The mean diameter of PCL/Gel/nHA/Vit D3 nanofibers was about 531 nm. The culture of osteoblast cells on the scaffolds showed that the addition of Vit D3 to PCL/Gel/nHA scaffold caused further attachment and proliferation of the cells. In addition, stained mineral deposits scaffolds with alizarin red staining showed that the amount of mineralized deposits was significantly higher in PCL/Gel/nHA/Vit D3 scaffold than other scaffolds (P < 0.05).Conclusion: Superior properties of nano-hydroxyapatite and vitamin D3 blended in polycaprolactone/gelatin-based scaffold were confirmed. The observations also revealed that the composite scaffold could be a good candidate for bone tissue engineering.

Keywords

References

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Journal of Isfahan Medical School
Volume 35, Issue 425 - Serial Number 425
March and April 2017
Pages 387-392

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History

  • Receive Date: 07 January 2017
  • Revise Date: 20 April 2024
  • Accept Date: 06 April 2022

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