Background: Klebsiella pneumoniae (K. pneumonia) is an opportunistic pathogen and one of the most common causes of nosocomial infections, some of which are now resistant to antibiotics and make infection difficult to treat. The main purpose of this study was to use multiplex polymerase chain reaction (PCR) to detect tetracycline-resistance genes in clinical isolates of K. pneumonia.Methods: After collecting clinical samples from hospitals in Mashhad City, Iran, in 2021, K. pneumoniae was isolated and identified by biochemical and molecular methods (Kp16S rRNA specific primer). Antibiotic-resistance pattern was determined for 30 isolates by disk diffusion method. After designing primer and its blast, detection of tet genes was optimized by PCR. Finally, multiplex PCR was set up using three pairs of primers and positive control.Findings: The highest and lowest antibiotic resistance of the isolates was for ampicillin (90%) and ceftriaxone (26.66%), respectively, and 53.33% of Klebsiella isolates were phenotypically resistant to tetracycline. Multiplex PCR was set up by annealing temperature of 55°C. Simultaneously with detection of Kp16S rRNA gene, tetA and tetB genes were detected in 87.5% and 68.75% of tetracycline-resistant Klebsiella isolates, respectively. 56.25% of the isolates carried both tetA and tetB genes, simultaneously, and 12.5% of the isolates transferred one of the tetA or tetB genes.Conclusion: The results show that the multiplex PCR technique designed in this study is a fast, accurate, and sensitive method for identifying antibiotic-resistant K. pneumoniae that can be used for clinical samples or in epidemiological studies.
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Naji-Hamad, M., & Nakhaei-Moghaddam, M. (2021). Detection of Tetracycline-Resistant Genes by Multiplex Polymerase Chain Reaction in Clinical Isolates of Klebsiella Pneumonia. Journal of Isfahan Medical School, 39(647), 815-820. doi: 10.22122/jims.v39i647.14366
MLA
Manar Naji-Hamad; Mahboobeh Nakhaei-Moghaddam. "Detection of Tetracycline-Resistant Genes by Multiplex Polymerase Chain Reaction in Clinical Isolates of Klebsiella Pneumonia". Journal of Isfahan Medical School, 39, 647, 2021, 815-820. doi: 10.22122/jims.v39i647.14366
HARVARD
Naji-Hamad, M., Nakhaei-Moghaddam, M. (2021). 'Detection of Tetracycline-Resistant Genes by Multiplex Polymerase Chain Reaction in Clinical Isolates of Klebsiella Pneumonia', Journal of Isfahan Medical School, 39(647), pp. 815-820. doi: 10.22122/jims.v39i647.14366
VANCOUVER
Naji-Hamad, M., Nakhaei-Moghaddam, M. Detection of Tetracycline-Resistant Genes by Multiplex Polymerase Chain Reaction in Clinical Isolates of Klebsiella Pneumonia. Journal of Isfahan Medical School, 2021; 39(647): 815-820. doi: 10.22122/jims.v39i647.14366
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