تأثیر تمرینات استقامتی و عصاره‌ی گزنه بر بیان ژن COX-1 و COX-2 در موش‌های مبتلا به سرطان ملانوما

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، آمل، ایران

2 دانشیار، گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

3 استادیار، گروه فیزیولوژی ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

مقاله پژوهشی




مقدمه: هدف از مطالعه‌ی حاضر، بررسی تأثیر تمرینات استقامتی و عصاره‌ی گزنه بر بیان ژن COX-1 و COX-2 در موش‌های مبتلا به سرطان ملانوما بود.
روش‌ها: در این مطالعه، 20 سر موش صحرایی نر بالغ به صورت تصادفی به 4 گروه تقسیم شدند: 1. شاهد، 2. تمرین بدنی، 3. عصاره‌ی گزنه، 4. تمرین بدنی + عصاره‌ی گزنه تقسیم شدند. یک هفته پس از القاء سرطان ملانوما، گروه تجربی میزان mg/kg/day30 عصاره‌ی اتانولی گیاه گزنه را به روش خوراکی و به مدت
8 هفته مصرف کردند. برنامه‌ی تمرین شامل 30 دقیقه دویدن روی تردمیل بدون شیب و با سرعت 16 متر در دقیقه برای هفته‌ی اول بود و هر هفته یک متر بر دقیقه اضافه شد تا در هفته‌ی هشتم به 22 متر بر دقیقه رسید. برای اندازه‌گیری میزان بیان ژن COX-1 و COX-2 از روش  RT PCRاستفاده شد.
یافته‌ها: مصرف عصاره‌ی گزنه و تمرینات استقامتی موجب تغییرات معنی‌دار سطوح COX-2 در گروه‌های تجربی در مقایسه با گروه شاهد شد. مقادیر COX-2 در گروه‌های عصاره و ترکیبی در مقایسه با گروه شاهد کاهش معنی‌داری یافت. همچنین نتایج نشان داد که سطوح COX-1 تغییرات معنی‌داری در بین گروه‌های تجربی و شاهد نداشت.
نتیجه‌گیری: نتایج مطالعه‌ی حاضر نشان داد، ترکیبات شیمیایی موجود در عصاره‌ی گزنه مانند فلاونوئیدها، ترپن‌ها، اسیدهای چرب و فنولیک‌ها ممکن است مسؤول اثر ضد آپوپتوز و ضدسرطانی باشند. همچنین تمرینات استقامتی سبب کاهش معنی‌دار در مقادیر COX2 نسبت به گروه شاهد شد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Nettle Extract and Aerobic Training on the Gene Expression of COX-1 and COX-2 in Mice with Melanoma

نویسندگان [English]

  • Javid Esmaeelpour 1
  • Alireza Barari 2
  • Ahmad Abdi 2
  • Hosein Abed-Natanzi 3
1 PhD Student in Sport Physiology, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 Associate Professor, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
3 Assistant Professor, Department of Sport Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Background: The aim of this study was to evaluate the effect of nettle extract consumption and aerobic exercise on COX-1and COX-2gene expression in mice with melanoma.
Methods: In this study, 20 adult male rats were randomly divided into 4 groups: 1. control, 2. physical exercise, 3. nettle extract, 4. physical exercise + nettle extract. A week after inducing melanoma, the experimental group consumed 30 mg / kg / day of nettle ethanol extract orally for 8 weeks. The training program consisted of 30 minutes of running on a treadmill without a slope at a speed of 16 meters per minute for the first week, and one meter per minute was added every week until it reached 22 meters per minute in the eighth week. RT PCR was used to measure the expression of COX-1and COX-2 genes.
Findings: The results of the present study showed that consumption of nettle extract and aerobic exercise caused significant changes in COX-2 levels in experimental groups compared with the control group. COX-2 levels in the extract and combination groups were significantly reduced compared to the control group. Also, the results showed that COX-1 levels were significantly different between the experimental and control groups.
Conclusion: The results of the present study showed that the chemical compounds in nettle extract such as flavonoids, terpenes, fatty acids and phenolics may be responsible for apoptotic and anti-cancer effects. Also, endurance training caused a significant decrease in COX2 levels compared with the control group.

کلیدواژه‌ها [English]

  • Endurance training
  • Cyclooxygenase
  • Prostaglandins
  • Flavonoids
  • Terpenes
  • Fatty acids

مراجع

  1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA Cancer J Clin 2019; 69(1): 7-3.
  2. Guy GP, Thomas CC, Thompson T, Watson M, Massetti GM, Richardson LC, et al. Vital signs: melanoma incidence and mortality trends and projections-United States, 1982-2030. MMWR Morb Mortal Wkly Rep 2015; 64(21): 591-6.
  3. Que J, Garman KS, Souza RF, Spechler SJ. Pathogenesis and cells of origin of barrett’s esophagus. Gastroenterology 2019; 157(2): 349-64.
  4. Moon H, Kim D, Donahue LR, White AC. Phenotypic plasticity of cutaneous squamous cell carcinoma mediated by cyclooxygenase-2. J Invest Dermatol 2020; 140(8):1665-9.
  5. Kaminska K, Szczylik C, Lian F, Czarnecka AM. The role of prostaglandin E2 in renal cell cancer development: Future implications for prognosis and therapy. Future Oncol 2014; 10(14): 2177-87.
  6. Elmets CA, Ledet JJ, Athar M. Cyclooxygenases: Mediators of UV-induced skin cancer and potential targets for prevention. J Invest Dermatol 2014; 134(10): 2497-502.
  7. Muraoka N, Nara K, Tamura F, Kojima H, Yamakawa H, Sadahiro T, et al. Role of cyclooxygenase-2-mediated prostaglandin E2- prostaglandin E receptor 4 signaling in cardiac reprogramming. Nat Commun 2019; 10(1): 674.
  8. de Thé H. Differentiation therapy revisited. Nat Rev Cancer 2018; 18(2): 117-27.
  9. Jiao J, Mikulec C, Ishikawa TO, Magyar C, Dumlao DS, Dennis EA, et al. Cell-type-specific roles for COX-2 in UVB-induced skin cancer. Carcinogenesis 2014; 35(6): 1310-9.
  10. Yari Khosroshahi A, Habibi Khaniani B, Naghdibadi HA. Review on taxol as the most important anticancer natural drug [In Persian]. J Med Plants 2006; 5(18): 1-10.
  11. Bourgeois C, Leclerc ÉA, Corbin C, Doussot J, Serrano V, Vanier JR, et al. Nettle (Urtica dioica) as a source of antioxidant and anti-aging phytochemicals for cosmetic applications. Comptes Rendus Chimie 2016; 19(9): 1090-100.
  12. Morgia G, Privitera S. Phytotherapy in benign prostatic hyperplasia. In: Morgia G, Russo GI, editors. Lower urinary tract symptoms and benign prostatic hyperplasia. Amsterdam, NA: Elsevier Science; 2018. p. 135-75.
  13. Fattahi S, Motevalizadeh Ardekani AM, Zabihi E, Abedian Z, Mostafazadeh A, Pourbagher R, et al. Antioxidant and apoptotic effects of an aqueous extract of Urtica dioica on the MCF-7 human breast cancer cell line. Asian Pac J Cancer Prev 2013; 14(9): 5317-23.
  14. Levy A, Sivanesan D, Murugan R, Jornadal J, Quinonez Y, Jaffe M, et al. Urtica dioica Induces cytotoxicity in human prostate carcinoma LNCaP Cells: Involvement of oxidative stress, mitochondrial depolarization and apoptosis. Trop J Pharm Res 2014; 13(5): 711-7.
  15. Mohammadi A, Mansoori B, Aghapour M, Shirjang S, Nami S, Baradaran B. The Urtica dioica extract enhances sensitivity of paclitaxel drug to MDA-MB-468 breast cancer cells. Biomed Pharmacother 2016; 83: 835-42.
  16. Barari AR, Mojhde MA, Farzanegi PA, Ghasemi MO. Effect of six weeks of endurance training and Aloe Vera on COX-2 and MMP-9 levels in mice with breast cancer [In Persian]. J Shahid Sadoughi Univ Med Sci 2016; 24(1): 65-73.
  17. Krüger K, Bredehöft J, Mooren FC, Rummel C. Different effects of strength and endurance exercise training on COX-2 and mPGES expression in mouse brain are independent of peripheral inflammation.
    J Appl Physiol (1985) 2016; 121(1): 248-54.
  18. Betof AS, Dewhirst MW, Jones LW. Effects and potential mechanisms of exercise training on cancer progression: a translational perspective. Brain Behav
    Immun 2013; 30(Suppl 0): S75-87.
  19. Beaudry RI, Liang Y, Boyton ST, Tucker WJ, Brothers RM, Daniel KM, et al. Meta-analysis of exercise training on vascular endothelial function in cancer survivors. Integr Cancer Ther 2018; 17(2): 192-9.
  20. Amjadi F, Haghjooy Javanmard S, Zarkesh-Esfahani H, Khazaei M, Narimani M. Leptin promotes melanoma tumor growth in mice related to increasing circulating endothelial progenitor cells numbers and plasma NO production. J Exp Clin Cancer Res 2011; 30(1): 21.
  21. Zhang M, Zhou S, Zhang L, Ye W, Wen Q, Wang J. Role of cancer-related inflammation in esophageal cancer. Crit Rev Eukaryot Gene Expr 2013, 23(1): 27-35.
  22. Cheng J, Fan XM. Role of cyclooxygenase-2 in gastric cancer development and progression. World J Gastroenterol 2013, 19(42): 7361-8.
  23. Elmets CA, Ledet JJ, Athar M. Cyclooxygenases: Mediators of UV-induced skin cancer and potential targets for prevention. J Invest Dermatol 2014; 134(10): 2497-502.
  24. Knab LM, Grippo PJ, Bentrem DJ. Involvement of eicosanoids in the pathogenesis of pancreatic cancer: The roles of cyclooxygenase-2 and 5-lipoxygenase. World J Gastroenterol 2014; 20(31): 10729-39.
  25. Cebola I, Peinado MA. Epigenetic deregulation of the COX pathway in cancer. Prog Lipid Res 2012; 51(4): 301-13.
  26. Renna NF, Diez ER, Lembo C, Miatello RM. Role of Cox-2 in vascular inflammation:an experimental model of metabolic syndrome. Mediators Inflamm 2013; 2013: 513251.
  27. Nam SM, Yi SS, Yoo KY, Park OK, Yan B, Song W, et al. Differential effects of treadmill exercise on cyclooxygenase-2 in the rat hippocampus at early and chronic stages of diabetes. Lab Anim Res 2011; 27(3): 189-95.
  28. Barrari A, Abbassi Daloii A, Tamaskan N. The effect of combining the aerobic exercise and silymarine on the cyclooxygenase-2 and coagulation factors in young inactive women. Indian J Fundam Appl Life Sci 2013, 3(3): 342-9.
  29. Samuels N, Morag O, Maimon Y. Use of herbal medicine for cancer treatment-related toxicities [In Hebrew]. Harefuah 2015; 154(1): 43-6, 67.
  30. Yin SY, Wei WC, Jian FY, Yang NS.Therapeutic applications of herbal medicines for cancer patients. Evid Based Complement Alternat Med 2013; 2013: 302426.
  31. Telo S, Halifeoglu I, Ozercan IH. Effects of stinging ettle (Urtica Dioica L.,) on antioxidant enzyme activities in rat model of mammary gland cancer. Iran J Pharm Res 2017; 16(Suppl): 164-70.
  32. Mohammadi A, Mansoori B, Chokhachi Baradaran P, Khaze V, Aghapour M, Farhadi M, et al. Urtica dioica extract inhibits proliferation and induces apoptosis and related gene expression of breast cancer cells in vitro and in vivo. Clin Breast Cancer 2017; 17(6): 463-70.
  33. Mansoori B, Mohammadi A, Hashemzadeh S, Shirjang S, Baradaran A, Asadi M, et al. Urtica dioica extract suppresses miR-21 and metastasis-related genes in breast cancer. Biomed Pharmacother 2017; 93: 95-102.
  34. Zhu Q, Jin L, Casero RA, Davidson NE, Huang Y. Role of ornithine decarboxylase in regulation of estrogen receptor alpha expression and growth in human breast cancer cells. Breast Cancer Res Treat 2012; 136(1): 57-66.
  35. Balunas MJ, Kinghorn AD. Drug discovery from medicinal plants. Life Sci 2005; 78(5): 431-41.
  36. Özkol H, Musa D, Tuluce Y, Koyuncu I. Ameliorative influence of Urtica dioica L against cisplatin-induced toxicity in mice bearing Ehrlich ascites carcinoma. Drug Chem Toxicol 2012; 35(3): 251-7.
  37. Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell 2011; 144(5): 646-74.
  38. Osman WM, Youssef NS. Combined use of COX-1 and VEGF immunohistochemistry refines the histopathologic prognosis of renal cell carcinoma. Int J Clin Exp Pathol 2015; 8(7): 8165-77.
  39. Anantharaju PG, Gowda PC, Vimalambike MG, Madhunapantula SV. An overview on the role of dietary phenolics for the treatment of cancers. Nutr J 20161; 15(1): 99.
مجله دانشکده پزشکی اصفهان
دوره 40، شماره 673
هفته 1 مردادماه
مرداد و شهریور 1401
صفحه 375-382

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سابقه مقاله

  • تاریخ دریافت: 09 مرداد 1401
  • تاریخ پذیرش: 09 مرداد 1401

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