مجله دانشکده پزشکی اصفهان

تأثیر تمرینات استقامتی و عصاره‌ی گزنه بر بیان ژن COX-1 و COX-2 در موش‌های مبتلا به سرطان ملانوما

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، آمل، ایران

2 دانشیار، گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

3 استادیار، گروه فیزیولوژی ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

مقاله پژوهشی




مقدمه: هدف از مطالعه‌ی حاضر، بررسی تأثیر تمرینات استقامتی و عصاره‌ی گزنه بر بیان ژن COX-1 و COX-2 در موش‌های مبتلا به سرطان ملانوما بود.
روش‌ها: در این مطالعه، 20 سر موش صحرایی نر بالغ به صورت تصادفی به 4 گروه تقسیم شدند: 1. شاهد، 2. تمرین بدنی، 3. عصاره‌ی گزنه، 4. تمرین بدنی + عصاره‌ی گزنه تقسیم شدند. یک هفته پس از القاء سرطان ملانوما، گروه تجربی میزان mg/kg/day30 عصاره‌ی اتانولی گیاه گزنه را به روش خوراکی و به مدت
8 هفته مصرف کردند. برنامه‌ی تمرین شامل 30 دقیقه دویدن روی تردمیل بدون شیب و با سرعت 16 متر در دقیقه برای هفته‌ی اول بود و هر هفته یک متر بر دقیقه اضافه شد تا در هفته‌ی هشتم به 22 متر بر دقیقه رسید. برای اندازه‌گیری میزان بیان ژن COX-1 و COX-2 از روش  RT PCRاستفاده شد.
یافته‌ها: مصرف عصاره‌ی گزنه و تمرینات استقامتی موجب تغییرات معنی‌دار سطوح COX-2 در گروه‌های تجربی در مقایسه با گروه شاهد شد. مقادیر COX-2 در گروه‌های عصاره و ترکیبی در مقایسه با گروه شاهد کاهش معنی‌داری یافت. همچنین نتایج نشان داد که سطوح COX-1 تغییرات معنی‌داری در بین گروه‌های تجربی و شاهد نداشت.
نتیجه‌گیری: نتایج مطالعه‌ی حاضر نشان داد، ترکیبات شیمیایی موجود در عصاره‌ی گزنه مانند فلاونوئیدها، ترپن‌ها، اسیدهای چرب و فنولیک‌ها ممکن است مسؤول اثر ضد آپوپتوز و ضدسرطانی باشند. همچنین تمرینات استقامتی سبب کاهش معنی‌دار در مقادیر COX2 نسبت به گروه شاهد شد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Nettle Extract and Aerobic Training on the Gene Expression of COX-1 and COX-2 in Mice with Melanoma

نویسندگان [English]

  • Javid Esmaeelpour 1
  • Alireza Barari 2
  • Ahmad Abdi 2
  • Hosein Abed-Natanzi 3

1 PhD Student in Sport Physiology, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

2 Associate Professor, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

3 Assistant Professor, Department of Sport Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

چکیده [English]

Background: The aim of this study was to evaluate the effect of nettle extract consumption and aerobic exercise on COX-1and COX-2gene expression in mice with melanoma.
Methods: In this study, 20 adult male rats were randomly divided into 4 groups: 1. control, 2. physical exercise, 3. nettle extract, 4. physical exercise + nettle extract. A week after inducing melanoma, the experimental group consumed 30 mg / kg / day of nettle ethanol extract orally for 8 weeks. The training program consisted of 30 minutes of running on a treadmill without a slope at a speed of 16 meters per minute for the first week, and one meter per minute was added every week until it reached 22 meters per minute in the eighth week. RT PCR was used to measure the expression of COX-1and COX-2 genes.
Findings: The results of the present study showed that consumption of nettle extract and aerobic exercise caused significant changes in COX-2 levels in experimental groups compared with the control group. COX-2 levels in the extract and combination groups were significantly reduced compared to the control group. Also, the results showed that COX-1 levels were significantly different between the experimental and control groups.
Conclusion: The results of the present study showed that the chemical compounds in nettle extract such as flavonoids, terpenes, fatty acids and phenolics may be responsible for apoptotic and anti-cancer effects. Also, endurance training caused a significant decrease in COX2 levels compared with the control group.

کلیدواژه‌ها [English]

  • Endurance training
  • Cyclooxygenase
  • Prostaglandins
  • Flavonoids
  • Terpenes
  • Fatty acids
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مجله دانشکده پزشکی اصفهان
دوره 40، شماره 673
مرداد 1401
صفحه 375-382
  • تاریخ دریافت: 09 مرداد 1401
  • تاریخ پذیرش: 09 مرداد 1401
  • تاریخ اولین انتشار: 09 مرداد 1401