Isolation and Molecular Identification of Candida Species Isolated from Patients with Onychomycosis by PCR-RFLP Method

Document Type : Original Article(s)

Authors

1 Associate Professor, Department of Medical Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 MSc Student, Department of Medical Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

3 Professor, Department of Parasitology and Mycology and Cutaneous Leishmaniosis Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4 Associate Professor, Department of Social Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

5 - MSc of Medical Parasitology, Department of Medical Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

6 MSc of Medical Parasitology, Department of Medical Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Background: Onychomycosis is the most common nail disorder, and Yeast is one of the most common causes of nail fungal infections. Now, the identification of yeast agents, especially Candida, has been increased as a pathogen responsible for nail infections. This study was designed to identify Candida species in patients with onychomycosis referred to diagnostic laboratories of Mashhad University Hospitals.
Methods: In the present study, 210 nail samples obtained from people suspected of onychomycosis were examined. After direct examination of the samples, some were cultured in fungal culture media, and Candida chrome agar medium was used for initial identification and obtaining pure cultures. Then, the Candida species were determined using the PCR-RFLP molecular method.
Findings: From 210 nail samples suspected of onychomycosis, 51 cases (24.2%) of Candida colonies were isolated, of which 14 cases (27.5%) were men and 37 cases (72.5%) were women, and in both sexes, those who were of 30-39 years old more infected. The most common Candida species isolated was Candida parapsilosis 23 cases (45.1%), followed by C. albicans 21 cases (41.2%), C. glabrata 2 cases (3.9%), C. tropicalis 2 cases (3.9%), C. guilliermondii 2 cases (3.9%). And there were 1 case (2%) of C. famata.
Conclusion: This study showed that Candida parapsilosis and Candida albicans are the most common species involved in the development of Candida onychomycosis.

Highlights

Ali Naseri: Google Scholar

Abdolmajid Fata: Google Scholar

Lida Jarahi: Google Scholar

Mahmoud Parian Noghani: Google Scholar

Asieh Fatemi Esfedan: Google Scholar

Keywords

Main Subjects

References

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Journal of Isfahan Medical School
Volume 42, Issue 772
4th Week, August
July and August 2024
Pages 511-518

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History

  • Receive Date: 12 May 2024
  • Accept Date: 05 October 2024

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