Effect of Continuous and Intermittent Aerobic Training on Serum Spexin and Insulin Sensitivity in Wistar Rats with Gestational Diabetes Mellitus: An Experimental Study

Document Type : Original Article (s)

Authors

1 PhD Student of Exercise Physiology, Department of Sport Sciences, University of Birjand, Iran

2 Professor of Exercise Physiology, Department of Sport Sciences, University of Birjand, Iran

3 Associate Professor, Department of Physiology, Zahedan University of Medical Sciences, Zahedan, Iran

4 Assistant Professor, Department of Physical Education and Sport Sciences, School of Human Sciences, University of Zabol, Zabol, Iran

Abstract

Background: Gestational Diabetes Mellitus (GDM) is any degree of glucose intolerance with onset or first recognition during pregnancy. Regular exercise is important for a healthy pregnancy and can lower the risk of developing GDM. On the other hand, the crux of the matter is to find a bio predictor capable of signaling out women at risk of developing GDM as early as the very start of pregnancy. This study aimed to present the potential and significance of Spexin (SPX) in the pathogenesis of GDM.
Methods: Sixty female Wistar rats (weighting 220 ± 10g) were randomly assigned into six groups: control (Ctr) group, control+continuous aerobic training (Ctr+CON) group, control+intermittent aerobic training (Ctr+INT) group, gestational diabetes mellitus (GDM) group, GDM+continuous aerobic training (GDM+CON) group, GDM+intermittent aerobic training (GDM+INT) group. Exercise groups underwent treadmill exercise during pregnancy. Levels of insulin sensitivity and spexin were measured. A one-way ANOVA statistical test was used for statistical analysis.
Findings: Serum spexin concentration in the GDM group was significantly higher than Ctr; on the other hand, the spexin level in the GDM+CON and GDM+INT groups was considerably lower than in the GDM group. On the 20th day of gestation, the weight of the GDM group was significantly higher than that of the Ctr and training groups. The birth weight of offspring in the GDM group was significantly higher than that of the Ctr and Exc groups. Insulin sensitivity levels in the GDM+CON and GDM+INT groups were significantly lower than in the GDM group.
Conclusion: The results of this study demonstrate that both continuous and intermittent training were effective in improving GDM, but there was no statistically significant difference between the two training models. In addition, Changes in SPX levels seem to be influenced by the presence of GDM in pregnant women, with increased levels in those with GDM. It may be used as a potential factor to predict the development of GDM.

Highlights

Ahmad Arbabi: Google Scholar ,PubMed

Mehdi Mogharnasi: Google Scholar ,PubMed 

Keywords

References

  1. Wang M, Chen X, Shang Y, Chen B, Chen H, Zhou L, et al. Oligopeptide-strategy of targeting at adipose tissue macrophages using ATS-9R/siCcl2 complex for ameliorating insulin resistance in GDM. Biomed Pharmacother 2024; 175: 116775.
  2. Liu Y, Li S, Qi X, Zhou W, Liu X, Lin H, et al. A novel neuropeptide in suppressing luteinizing hormone release in goldfish, Carassius auratus. Mol Cell Endocrinol 2013; 374(1-2): 65-72.
  3. Wu H, Lin F, Chen H, Liu J, Gao Y, Zhang X, et al. Ya-fish (Schizothorax prenanti) spexin: identification, tissue distribution and mRNA expression responses to periprandial and fasting. Fish Physiol Biochem 2016; 42: 39-49.
  4. Ma A, Bai J, He M, Wong AO. Spexin as a neuroendocrine signal with emerging functions. Gen Comp Endocrinol 2018; 265: 90-6.
  5. Al-Daghri NM, Alenad A, Al-Hazmi H, Amer OE, Hussain SD, Alokail MS. Spexin levels are associated with metabolic syndrome components. Dis Markers 2018; 2018(1): 1679690.
  6. Karaca A, Bakar-Ates F, Ersoz-Gulcelik N. Decreased spexin levels in patients with type 1 and type 2 diabetes. Med Princ Pract 2019; 27(6): 549-54.
  7. Akbas M, Koyuncu FM, Mete TO, Taneli F, Ozdemir H, Yilmaz O. Serum levels of spexin are increased in the third trimester pregnancy with gestational diabetes mellitus. Gynecol Endocrinol 2019; 35(12): 1050-3.
  8. Simsir C, Kalem MN, Kalem Z, Var T, Bakirarar B, Coskun B, et al. Circulating Spexin levels in pregnant women with and without gestational diabetes. Int J Reprod Contracept Obstet Gynecol 2019; 8(10): 4056-62.
  9. Behrasi F, Khayat S, Karajibani M, Fanaei H, Montazerifar F. Association between spexin, nutritional status of pregnant women with excessive gestational weight gain, and newborn weight: A cross-sectional study. Clinical Nutrition Open Science 2024; 55: 79-90.
  10. Shin M-S, Kim H, Chang H-K, Lee T-H, Jang M-H, Shin M-C, et al. Treadmill exercise suppresses diabetes-induced increment of neuropeptide Y expression in the hypothalamus of rats. Neurosci Lett 2003; 346(3): 157-60.
  11. Al-Daghri NM, Wani K, Yakout SM, Al-Hazmi H, Amer OE, Hussain SD, et al. Favorable changes in fasting glucose in a 6-month self-monitored lifestyle modification programme inversely affects spexin levels in females with prediabetes. Sci Rep 2019; 9(1): 9454.
  12. Mohammadi A, Bijeh N, Moazzami M, khodaei K, Rahimi N. Effect of exercise training on spexin level, appetite, lipid accumulation product, visceral adiposity index, and body composition in adults with type 2 diabetes. Biol Res Nurs 2022; 24(2): 152-62.
  13. Leciejewska N, Pruszyńska-Oszmałek E, Mielnik K, Głowacki M, Lehmann TP, Sassek M, et al. Spexin Promotes the Proliferation and Differentiation of C2C12 Cells In Vitro—The Effect of Exercise on SPX and SPX Receptor Expression in Skeletal Muscle In Vivo. Genes 2021; 13(1): 81.
  14. Khadir A, Kavalakatt S, Madhu D, Devarajan S, Abubaker J, Al-Mulla F, et al. Spexin as an indicator of beneficial effects of exercise in human obesity and diabetes. Sci Rep 2020; 10(1): 10635.
  15. Mahizir D, Briffa JF, Wood JL, Anevska K, Hill‐Yardin EL, Jefferies AJ, et al. Exercise improves metabolic function and alters the microbiome in rats with gestational diabetes. FASEB J 2020; 34(1): 1728-44.
  16. Wang C, Guelfi KJ, Yang H-X. Exercise and its role in gestational diabetes mellitus. Chronic Dis Transl Med 2016; 2(04): 208-14.
  17. Fang P, Ge R, She Y, Zhao J, Yan J, Yu X, et al. Adipose tissue spexin in physical exercise and age-associated diseases. Ageing Res Rev 2022; 73: 101509.
  18. Physical Activity and Exercise During Pregnancy and the Postpartum Period: ACOG Committee Opinion, Number 804. Obstet Gynecol 2020; 135(4): e178-e188.
  19. Huang X, Huang J, Wu J, Li M, Yang Z, Liu L, et al. Different exercises for pregnant women with gestational diabetes: a meta-analysis of randomized controlled trials. J Sports Med Phys Fitness 2019; 60( 3): 464-71.
  20. Roberts CK, Won D, Pruthi S, Barnard RJ. Effect of a diet and exercise intervention on oxidative stress, inflammation and monocyte adhesion in diabetic men. Diabetes Res Clin Pract 2006; 73(3): 249-59.
  21. Chen F, Ge L, Jiang X, Lai Y, Huang P, Hua J, et al. Construction of the experimental rat model of gestational diabetes. PLoS One 2022; 17(9): e0273703.
  22. Ayyoubi A, Mood MP, Hafezinori H, Nakhaei H, Fanaei H. Treadmill exercise during pregnancy decreases serum asprosin in rats with gestational diabetes mellitus. Obesity Medicine 2023; 42: 100511.
  23. Wang X, Stridh L, Li W, Dean J, Elmgren A, Gan L, et al. Lipopolysaccharide sensitizes neonatal hypoxic-ischemic brain injury in a MyD88-dependent manner. J Immunol 2009; 183(11): 7471-7.
  24. Gorgij E, Fanaei H, Yaghmaei P, Shahraki MR, Mirahmadi H. Treadmill exercise during pregnancy decreased vulnerability to neonatal hypoxia-ischemia through reducing inflammation and increasing antiapoptotic gene expressions and antioxidant capacity in rats. Stroke Res Treat 2021; 5512745.
  25. Afzalpour ME, Mogharnasi M, Zahraei H, Amirabadizadeh M, Nakhaie H. Comparison of Eight Weeks of Continuous and High Intensity Interval Swimming on Irisin Tissue Levels and Insulin Sensitivity in Male Rats with Metabolic Syndrome [in Persian]. Med J Mashhad Univ Med Sci 2023; 65(5): 1925-41.
  26. Yavuzkir S, Ugur K, Deniz R, Ustebay DU, Mirzaoglu M, Yardim M, et al. Maternal and umbilical cord blood subfatin and spexin levels in patients with gestational diabetes mellitus. Peptides 2020; 126: 170277.
  27. Lim CH, Lee MYM, Soga T, Parhar I. Evolution of structural and functional diversity of spexin in mammalian and non-mammalian vertebrate species. Front Endocrinol (Lausanne) 2019; 10: 379.
  28. Gu L, Ma Y, Gu M, Zhang Y, Yan S, Li N, et al. Spexin peptide is expressed in human endocrine and epithelial tissues and reduced after glucose load in type 2 diabetes. Peptides 2015; 71: 232-9.
  29. Gu L, Ding X, Wang Y, Gu M, Zhang J, Yan S, et al. Spexin alleviates insulin resistance and inhibits hepatic gluconeogenesis via the FoxO1/PGC-1α pathway in high-fat-diet-induced rats and insulin resistant cells. Int J Biol Sci 2019; 15(13): 2815.
  30. Yu M, Ju M, Fang P, Zhang Z. Emerging central and peripheral actions of spexin in feeding behavior, leptin resistance and obesity. Biochem Pharmacol 2022; 202: 115121.
  31. Sassek M, Kolodziejski PA, Szczepankiewicz D, Pruszynska-Oszmalek E. Spexin in the physiology of pancreatic islets—mutual interactions with insulin. Endocrine 2019; 63: 513-9.
  32. Tanday N, Lafferty RA, Flatt PR, Irwin N. Beneficial metabolic effects of recurrent periods of beta‐cell rest and stimulation using stable neuropeptide Y1 and glucagon‐like peptide‐1 receptor agonists. Diabetes Obes Metab 2022; 24(12): 2353-63.
  33. Lee Y-N, Reyes-Alcaraz A, Yun S, Lee CS, Hwang J-I, Seong JY. Exploring the molecular structures that confer ligand selectivity for galanin type II and III receptors. PloS One 2020; 15(3): e0230872.
  34. Wong MK, Chen Y, He M, Lin C, Bian Z, Wong AO. Mouse spexin:(II) functional role as a satiety factor inhibiting food intake by regulatory actions within the hypothalamus. Front Endocrinol (Lausanne) 2021; 12: 681647.
  35. Kolodziejski PA, Pruszynska-Oszmalek E, Micker M, Skrzypski M, Wojciechowicz T, Szwarckopf P, et al. Spexin: A novel regulator of adipogenesis and fat tissue metabolism. Biochim Biophys Acta Mol Cell Biol Lipids 2018; 1863(10): 1228-36.
  36. Kolodziejski PA, Leciejewska N, Chmurzynska A, Sassek M, Szczepankiewicz A, Szczepankiewicz D, et al. 30-Day spexin treatment of mice with diet-induced obesity (DIO) and type 2 diabetes (T2DM) increases insulin sensitivity, improves liver functions and metabolic status. Mol Cell Endocrinol 2021; 536: 111420.
  37. Kolahdouzi S, Talebi-Garakani E, Hamidian G, Safarzade A. Exercise training prevents high-fat diet-induced adipose tissue remodeling by promoting capillary density and macrophage polarization. Life Sci 2019; 220: 32-43.
  38. Kleinert M, Parker BL, Jensen TE, Raun SH, Pham P, Han X, et al. Quantitative proteomic characterization of cellular pathways associated with altered insulin sensitivity in skeletal muscle following high-fat diet feeding and exercise training. Sci Rep 2018; 8(1): 10723.
Journal of Isfahan Medical School
Volume 42, Issue 787
3rd Week, December
November and December 2024
Pages 892-902

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History

  • Receive Date: 01 October 2024
  • Accept Date: 24 November 2024

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