The Effect of Water Training on Cytochrome C and P53 Expression in Heart Muscle Tissue of Rats Treated with Doxorubicin

Document Type : Original Article (s)

Authors

1 PhD Student, Department of Sport Physiology, School of Sport Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Professor, Department of Sport Physiology, School of Sport Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Associate Professor, Department of Basic Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Associate Professor, Department of Sport Physiology, School of Sport Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Background: Doxorubicin (DOX) is a widely used drug in cancer chemotherapy, but its cardiotoxicity limits its clinical applications. Combining exercise with chemotherapy offers a promising approach to reducing the side effects of chemotherapy drugs. Limited information is available on the effects of water training on the molecular mechanisms involved in DOX cardiotoxicity. This study aimed to investigate the modulating effect of water training on cytochrome c and p53 expression in heart muscle tissue of rats treated with doxorubicin.
Methods: 32 adult male Wistar rats (weight 200-220 g) were randomly divided into four groups: control (C), DOX (D), (intraperitoneal injection of 5 mg/kg DOX, once a week, for 5 weeks), water training (S) were divided water training (S); (for 60 minutes a day, five days a week, for six weeks) and DOX group with water training (DS). At the end of the study, the cardiac expression of p53 and Cytc proteins were analyzed using western blot and ELISA methods, respectively, and data analysis was done with one-way ANOVA.
Findings: The results showed that the administration of DOX caused a significant increase in the expression level of variable p53 and cytochrome c (Cytc) in the DOX-treated group compared to the control group (P < 0.0001). Water training led to a significant decrease in cardiac expression of p53 and Cytc proteins in the group treated with DOX (P < 0.001, P < 0.01).
Conclusion: Our findings suggest water training as a therapeutic strategy to reduce DOX-related cardiac damage by modulating p53 and Cytc proteins.

Highlights

Hasan Darvakh: Google Scholar 

Saied Shakerian:Google Scholar, PubMed

Mohammad Reza Tabandeh: Google Scholar, PubMed

Rohollah Ranjba: Google Scholar

Keywords

Main Subjects

References

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Journal of Isfahan Medical School
Volume 42, Issue 794
2nd Week, February
January and February 2025
Pages 1069-1076

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History

  • Receive Date: 28 May 2024
  • Accept Date: 26 January 2025

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