بررسی فراوانی اینتگرون 1، 2 و تشکیل بیوفیلم در جدایه‌های بالینی سودوموناس آئروژینوزا شهر تهران

نوع مقاله : Original Article(s)

نویسندگان

1 دانشجوی دکتری گروه میکروبیولوژی، دانشکده‌ی علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران

2 استاد، گروه میکروبیولوژی، مرکز تحقیقات آزمایشگاهی مرجع سلامت ایران، وزارت بهداشت، درمان و آموزش پزشکی، تهران، ایران

3 دانشیار، گروه میکروبیولوژی، دانشکده‌ی علوم و فناوری زیستی، دانشگاه شهید بهشتی، تهران، ایران

چکیده

مقدمه: سودوموناس آئروژینوزا، یکی از باکتری‌های مهم در ایجاد طیف گسترده‌ای از عفونت‌ها می‌باشد.گسترش شیوع مقاومت آنتی‌بیوتیک در میان آنها نگران‌کننده بوده و اینتگرون‌ها در کنار تشکیل بیوفیلم از عوامل مهم افزایش بروز مقاومت آنتی‌بیوتیک در آنها هستند. در مطالعه‌ی حاضر، فراوانی اینتگرون‌های کلاس 1، 2 و تشکیل بیوفیلم در بین جدایه‌های بالینی سودوموناس آئروژینوزا در شهر تهران بررسی گردید.
روش‌ها: در این مطالعه، 100 ایزوله بالینی سودوموناس آئروژینوزا جداسازی شد. نمونه‌ها از نظر کشت میکروبی و بیوشیمیایی بررسی و تأیید مولکولی با PCR برای ژن‌های OprL انجام و حضور اینتگرون‌های کلاس 1 و 2 بررسی شد.
یافته‌ها: شیوع اینتگرون کلاس 1 و 2 به ترتیب 47 و 8 درصد بود. اینتگرون 1 و 2 بطور معنی‌داری در بیوفیلم شدید و متوسط بیشتر از موارد ضعیف بودند (05/0 > P). از 33 نمونه با بیوفیلم قوی 9/90 درصد نمونه‌ها دارای ژن اینتگرون 1 بودند. نتایج نشان داد که ژن اینتگرون 1 بطور معنی‌داری در نمونه‌های با مقاومت دارویی چندگانه (Multiple drug resistance) MDR بیشتر از نمونه‌های فاقد MDR بود (05/0 > P).
نتیجه‌گیری: ارتباط بین تشکیل بیوفیلم و حضور اینتگرون‌ها نشان‌دهندهی‌ نقش این عوامل در ایجاد مقاومت آنتی‌بیوتیکی بوده و حضور اینتگرون‌های 1 و 2 در سویه‌های بالینی می‌تواند ریسک انتقال ژن‌های مقاومت دارویی را افزایش دهد.

تازه های تحقیق

سعید بشارتی:  Google Scholar, PubMed

محمد رهبر: Google Scholar

ندا سلیمانی: Google Scholar, PubMed

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Evaluation of the Prevalence of Integron 1, 2 and Biofilm Formation in Clinical Isolates of Pseudomonas Aeruginosa in Tehran

نویسندگان [English]

  • Saeid Besharati 1
  • Mohammad Rahbar 2
  • Neda Soleimani 3
1 PhD Student, Department of Microbiology and Microbial Biotechnology, School of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2 Professor, Departments of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran
3 Assistant Professor of Medical Bacteriology, Department of Microbiology and Microbial Biotechnology, School of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
چکیده [English]

Background: Pseudomonas aeruginosa is a significant bacterium responsible for various infections. The increasing spread of antibiotic resistance in these bacteria is concerning. Key factors contributing to the rise in antibiotic resistance include the presence of integrons and the ability to form biofilms. The current study investigated the abundance of class 1 and 2 integrons and biofilm formation among clinical isolates of Pseudomonas aeruginosa in Tehran, Iran.
Methods: In this study, 100 clinical isolates of Pseudomonas aeruginosa were isolated. The samples were examined in terms of microbial and biochemical culture, molecular confirmation was done by PCR for OprL genes and the presence of class 1 and 2 integrons was checked. The significance of variables was checked with the p-value.
Findings: The prevalence of integron class 1 and 2 was 47% and 8%, respectively. Integron 1 and 2 were significantly higher in severe and moderate biofilm than in weak cases (P < 0.05). Out of 33 samples with strong biofilm, 90.9% of samples had the integron one gene. The results showed that the integron one gene was significantly higher in Multiple drug resistance (MDR) samples than in non-MDR samples (P < 0.05).
Conclusion: The relationship between biofilm formation and the presence of integrons shows the role of these factors in creating antibiotic resistance, and the presence of integrons 1 and 2 in clinical strains can increase the risk of drug resistance gene transmission.

کلیدواژه‌ها [English]

  • Pseudomonas aeruginosa
  • Drug resistance
  • Biofilms
  • Integrons

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مجله دانشکده پزشکی اصفهان
دوره 43، شماره 805
هفته 1، اردیبهشت
فروردین و اردیبهشت 1404
صفحه 125-134

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سابقه مقاله

  • تاریخ دریافت: 26 آبان 1403
  • تاریخ پذیرش: 28 بهمن 1403

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