Evaluation of the Prevalence of Integron 1, 2 and Biofilm Formation in Clinical Isolates of Pseudomonas Aeruginosa in Tehran

Document Type : Original Article(s)

Authors

1 PhD Student, Department of Microbiology and Microbial Biotechnology, School of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

2 Professor, Departments of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran

3 Assistant Professor of Medical Bacteriology, Department of Microbiology and Microbial Biotechnology, School of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

Abstract

Background: Pseudomonas aeruginosa is a significant bacterium responsible for various infections. The increasing spread of antibiotic resistance in these bacteria is concerning. Key factors contributing to the rise in antibiotic resistance include the presence of integrons and the ability to form biofilms. The current study investigated the abundance of class 1 and 2 integrons and biofilm formation among clinical isolates of Pseudomonas aeruginosa in Tehran, Iran.
Methods: In this study, 100 clinical isolates of Pseudomonas aeruginosa were isolated. The samples were examined in terms of microbial and biochemical culture, molecular confirmation was done by PCR for OprL genes and the presence of class 1 and 2 integrons was checked. The significance of variables was checked with the p-value.
Findings: The prevalence of integron class 1 and 2 was 47% and 8%, respectively. Integron 1 and 2 were significantly higher in severe and moderate biofilm than in weak cases (P < 0.05). Out of 33 samples with strong biofilm, 90.9% of samples had the integron one gene. The results showed that the integron one gene was significantly higher in Multiple drug resistance (MDR) samples than in non-MDR samples (P < 0.05).
Conclusion: The relationship between biofilm formation and the presence of integrons shows the role of these factors in creating antibiotic resistance, and the presence of integrons 1 and 2 in clinical strains can increase the risk of drug resistance gene transmission.

Highlights

Saeid Besharati: Google Scholar, PubMed

Mohammad Rahbar: Google Scholar

Neda Soleimani: Google Scholar, PubMed

Keywords

Main Subjects

References

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Ghazaei C. Pseudomonas aeruginosa: Prevalence of Pathogenic Genes, OprL and ToxA in Human and Veterinary Clinical Samples in Ardabil, Iran, 2020. Journal of Advanced Biomedical Sciences 2022; 12(4): 412-21.

Journal of Isfahan Medical School
Volume 43, Issue 805
1st Week, May
May and June 2025
Pages 125-134

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History

  • Receive Date: 16 November 2024
  • Accept Date: 16 February 2025

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