تأثیر تمرین هوازی و مصرف ملاتونین بر بیان ژن برخی از عوامل التهابی و شاخص‌های آنتی‌اکسیدانی در بافت کبد رت‌های نر دیابتی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، واحد شوشتر، دانشگاه آزاد اسلامی، شوشتر، ایران

2 گروه علوم ورزشی، واحد مسجد سلیمان، دانشگاه آزاد اسلامی، مسجد سلیمان، ایران

3 گروه فیزیولوژی ورزشی، دانشکده‌ی علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران

10.48305/jims.v43.i808.0244

چکیده

مقاله پژوهشی




مقدمه: دیابت، به عنوان یک بیماری سیستمیک می‌تواند با افزایش گونه‌های واکنش‌پذیر منجر به آسیب‌های بافتی و کاهش عملکرد کبد شود. بنابراین هدف از انجام مطالعه‌ی حاضر، بررسی تأثیر تمرین هوازی و مصرف ملاتونین بر بیان ژن برخی از عوامل التهابی و شاخص‌های آنتی‌اکسیدانی در بافت کبد رت‌های نر دیابتی می‌باشد.
روش‌ها: در این پژوهش تجربی، 30 سر رت نژاد ویستار 8 هفته‌ای به‌ صورت تصادفی انتخاب و 24 سر از آنها جهت القای دیابت با تزریق محلول استروپتوزوتوسین (یک دوز 55 میلی‌گرم) دیابتی شدند. رت‌های دیابتی در 4 گروه شامل دیابت شاهد (DC)، دیابت ‌ملاتونین (DMel)، دیابت ‌تمرین‌هوازی (DEx)، دیابت ‌تمرین‌هوازی+ملاتونین (DEx+Mel) تقسیم شدند. یک گروه سالم شاهد (HC) نیز در نظر گرفته شد. برنامه‌ی تمرین هوازی شامل 5 روز در هفته و به مدت 6 هفته بود. داده‌ها از طریق آزمون‌های تحلیل واریانس یک‌طرفه و تعقیبی Tukey آنالایز شد. سطح معنی‌داری 0/05P ≤  در نظر گرفته شد.
یافته‌ها: انجام تمرین هوازی و مصرف ملاتونین منجر به کاهش معنی‌داری در بیان ژن IL-18 (0/001 = P) و IL-1β (0/005 = P) شد. از طرفی منجر به افزایش معنی‌داری در مقادیر SOD، GPx، TAC و کاهش معنی‌داری در مقادیر MDA در گروه‌های مداخله مشاهده شد (0/001 = P).
نتیجه‌گیری: نتایج نشان‌دهنده‌ی افزایش دفاع آنتی‌اکسیدانی و کاهش عوامل التهابی بود. بر اساس نتایج این مطالعه پیشنهاد داده می‌شود انجام تمرین ورزشی هوازی همراه با مصرف ملاتونین نتایج سودمندتر و بهتری نسبت به تمرین ورزشی هوازی و مصرف ملاتونین به صورت جداگانه دارد.

تازه های تحقیق

شیرین زیلائی‌بوری:  PubMed , Google Scholar 

فرهاد دریانوش:  PubMed , Google Scholar

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Aerobic Training and Melatonin Consumption on Gene Expression of Some Inflammatory Factors and Antioxidant Index in Liver Tissue of Male Rats Diabetes

نویسندگان [English]

  • Yousef Falamarzi 1
  • Meysam Shabaaninia 1
  • Shirin Zilaei Bouri 2
  • Farhad Daryanoosh 3
1 Department of Physical Education & Sport Sciences, Sh.C., Islamic Azad University, Shoushtar, Iran
2 Department of Sport Sciences, MaS.C., Islamic Azad University, MasjedSoleiman, Iran
3 Department of Sport Sciences, School of Education and Psychology, Shiraz university, Shiraz, Iran
چکیده [English]

Background: Diabetes, as a systemic disease, can lead to tissue damage and decreased liver function by increasing reactive species. Therefore, the purpose of this research is to investigate the interactive effects of aerobic training and melatonin consumption on the gene expression of some inflammatory factors and antioxidant markers in the liver tissue of male Diabetic Rats.
Methods: In this experimental study, 30 eight-week-old Wistar rats were randomly selected and Diabetes was induced in 24 rats via a single injection of streptozotocin (55 mg/kg). Diabetic rats were divided into 4 groups including diabetes control (DC), diabetes melatonin (DMel), diabetes aerobic training (DEx), diabetes aerobic training+melatonin (DEx+Mel). A healthy control group (HC) was also considered. The aerobic training program consisted of 5 days a week, for 6 weeks. The data were analyzed using one-way analysis of variance and Tukey's post-hoc statistical tests in SPSS version 29 software, with significance set at P ≤ 0.05.
Findings: Aerobic training and melatonin intake resulted in a significant decrease in IL-18 (P = 0.001) and IL-1β
(P = 0.005) gene expression. On the other hand, it resulted in a significant increase in SOD, GPx, TAC values and a significant decrease in MDA values in the intervention groups (P = 0.001).
Conclusion: Results indicate enhanced antioxidant defense and reduced inflammatory factors. Based on the results of this study, it is suggested that aerobic training combined with melatonin intake is more beneficial than aerobic training and melatonin intake separately.

کلیدواژه‌ها [English]

  • Aerobic Training
  • Antioxidant indices
  • Diabetes
  • Inflammatory factors
  • Liver
  • Melatonin

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مجله دانشکده پزشکی اصفهان
دوره 43، شماره 808
هفته 4، اردیبهشت
فروردین و اردیبهشت 1404
صفحه 244-254

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سابقه مقاله

  • تاریخ دریافت: 19 آبان 1403
  • تاریخ پذیرش: 15 اردیبهشت 1404

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