اثر تمرین مقاومتی بر بیان ژن‌های FOXO3, MuRF1 و MAFbx در عضله‌ی نعلی موش‌های صحرایی بعد از 2 هفته تعلیق اندام تحتانی

نوع مقاله : Original Article(s)

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه ولی عصر رفسنجان، رفسنجان، ایران

2 دانشیار، گروه علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه ولی عصر رفسنجان، رفسنجان، ایران

3 استادیار، گروه علوم ورزشی، دانشکده‌ی ادبیات و علوم انسانی، دانشگاه ولی عصر رفسنجان، رفسنجان، ایران

10.48305/jims.v43.i818.0623

چکیده

مقاله پژوهشی




مقدمه: دوره‌های طولانی مدت عدم ‌فعالیت یا تعلیق مکانیکی می‌تواند منجر به از دست‌ دادن قابل توجه توده و قدرت عضلات اسکلتی شود. در پژوهش حاضر، اثر 4 هفته تمرین مقاومتی بر تغییرات بیان ژن‌های FOXO3, MuRF1 و MAFbx درگیر در آتروفی عضلانی پس از یک دوره تعلیق در عضله‌ی نعلی موش‌های صحرایی ‌نر بررسی شد.
روش‌ها: تعداد 32 سر موش صحرایی‌نر ویستار به صورت تصادفی به گروه‌های معلق، باز تمرین، بی‌تمرین، و تمرینی تقسیم شدند. گروه‌های تمرینی به مدت 4 هفته و هر هفته 3 جلسه تمرین داشتند. تمرین مقاومتی به صورت صعود به‌همراه وزنه متصل به دم حیوانات از یک نردبان عمودی انجام شد. پس از 48 ساعت از آخرین جلسه تمرین، عضله‌ی نعلی استخراج و میزان سطوح بیان ژن‌ها با تکنیک Real-Time PCR سنجیده شد. تجزیه و تحلیل داده‌ها تعیین تفاوت میان متغیرهای پژوهش با استفاده از آزمون‌های تحلیل واریانس یک‌راهه و آزمون تعقیبی Tukey در سطح معنی‌داری، 05/0 ˂ P تجزیه و تحلیل شدند.
یافته‌ها: یافته‌ها نشان داد بیان ژن‌های FOXO3, MuRF1 و MAFbx بطور معنی‌داری در اثر تمرین مقاومتی در عضله‌ی نعلی موش‌های صحرایی‌نر بویژه در گروه بازتمرینی نسبت به سایر گروه‌ها کاهش می‌یابد (به ترتیب 0/0001 = P، 0/0001 = P و 0/0001 = P).
نتیجه‌گیری: به نظر می‌رسد تمرین مقاومتی قبل و بعد از تعلیق اندام تحتانی، سبب کاهش آتروفی در عضله‌ی نعلی موش‌های صحرایی ‌نر می‌شود، با این حال گروه بازتمرینی نسبت به آتروفی مقاوم‌تر هستند. از طرفی تعلیق اندام تحتانی بیان ژن‌های آترفیک (FOXO3, MuRF1 و MAFbx) در عضله‌ی نعلی موش‌ها را افزایش می‌دهد.

تازه های تحقیق

عبدارضا کاظمی:  Google Scholar

 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Resistance Training on the Expression of FOXO3, Murf1 and Mafbx Genes in the Soleus Muscle of Rats after 2 Weeks of Lower Limb Suspension

نویسندگان [English]

  • Mohammad Manizadeh 1
  • Abdolreza Kazemi 2
  • Hamideh Abdolzadeh 3
  • Hossein Babaei 3
  • Vahid Ghanbari Mazidi 1
1 MSc of Sports Physiology, Department of Sports Sciences, Faculty of Literature and Human Sciences, Vali Asr University of Rafsanjan, Rafsanjan, Iran
2 Associated Professor, Department of Physical Education, Faculty of Letters and Humanities, Vali E-Asr University, Rafsanjan, Iran.
3 Assistant Professor, Department of Physical Education, Faculty of Letters and Humanities, Vali E-Asr University, Rafsanjan, Iran
چکیده [English]

Background: Long-term periods of inactivity or mechanical unloading can lead to significant loss of skeletal muscle mass and strength. In the present study, the effect of 4 weeks of resistance training on the expression changes of FOXO3, MuRF1 and MAFbx genes involved in muscle atrophy after a period of suspension in the soleus muscle of male rats was investigated.
Methods: Thirty-two male Wistar rats were randomly divided into four groups: suspended, retraining, detraining, and training. The training groups exercised 3 sessions per week for 4 weeks. Resistance training was performed by climbing a vertical ladder with a weight attached to the animals' tails. Forty-eight hours after the last training session, the soleus muscle was extracted and the gene expression levels were measured by Real-Time PCR technique. Data analysis was done with the difference between research variables was determined using one-way ANOVA tests and Tukey's post hoc test at a significance level of P ˂ 0.05.
Findings: The findings showed that the expression of FOXO3, MuRF1, and MAFbx genes significantly decreased due to resistance training in the soleus muscle of male rats, especially in the retraining group compared to other groups (P = 0.001, P = 0.001, and P = 0.001, respectively).
Conclusion: It seems that resistance training before and after lower limb suspension reduces atrophy in the soleus muscle of male rats, however, the retraining group is more resistant to atrophy. On the other hand, lower limb suspension increases the expression of atrophic genes (FOXO3, MuRF1 and MAFbx) in the soleus muscle of rats.

کلیدواژه‌ها [English]

  • Resistance training
  • Muscle atrophy
  • Gene expression

مراجع

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مجله دانشکده پزشکی اصفهان
دوره 43، شماره 818
هفته 2، مرداد
مرداد و شهریور 1404
صفحه 623-631

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  • تاریخ دریافت: 22 آبان 1403
  • تاریخ پذیرش: 11 خرداد 1404

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