نوع مقاله : Original Article(s)
نویسندگان
1 استادیار، گروه زیستشناسی، دانشکدهی علوم پایه، دانشگاه ایلام، ایلام، ایران.
2 دانشیار، گروه بیوتکنولوژی دارویی، دانشکدهی داروسازی، دانشگاه علوم پزشکی کرمان، کرمان، ایران.
3 استادیار، مرکز تحقیقات گوارش و کبد، دانشگاه علوم پزشکی کرمان، کرمان، ایران.
چکیده
تازه های تحقیق
سهیلا عرفانی: Google Scholar
باقر امیرحیدری: Google Scholar
سیده مهدیه خوش نظر: Google Scholar
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background: Cerebral ischemia-reperfusion is a major cause of increased oxidative stress in the brain, which leads to cellular damage and impaired normal function. Antioxidant compounds can counteract the damaging mechanisms associated with ROS. This study investigated the effect of (S)-Ketamine on reducing oxidative stress and its associated damage in a rat model of cerebral ischemia-reperfusion.
Methods: 28 male Wistar rats weighing 250-300 grams were randomly divided into four groups: control, ischemia-saline, ischemia-low dose esketamine, and ischemia-high dose esketamine. Ischemia was induced by occluding both common carotid arteries (CCA) for 20 minutes. Esketamine was injected immediately after ischemia at doses of 0.1 and 0.25 mg per kg body weight. The activity of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), was assessed using ELISA, while lipid peroxidation was evaluated using the thiobarbituric acid method.
Findings: MDA levels, as an indicator of oxidative stress, were higher in the CA1 region of the hippocampus in ischemic rats, while in the (S)-Ketamine-treated groups, the activities of antioxidant enzymes SOD, CAT, and GPx significantly increased and MDA levels decreased, indicating an increase in antioxidant capacity and a decrease in oxidative stress caused by ischemia-reperfusion in these groups.
Conclusion: (S)-Ketamine may play a crucial role in mitigating cerebral ischemia-reperfusion injury by reducing oxidative stress and enhancing antioxidant enzyme activity.
کلیدواژهها [English]