Antioxidant Therapeutic Potential of S-Ketamin Against Cerebral Ischemia/Reperfusion Injury in Male Rats

Document Type : Original Article(s)

Authors

1 Assistant Professor, Department of Biology, Faculty of Science, Ilam University, Ilam, Iran

2 Associate Professor, Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

3 Assistant Professor, Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

10.48305/jims.v43.i821.0749

Abstract

Background: Cerebral ischemia-reperfusion is a major cause of increased oxidative stress in the brain, which leads to cellular damage and impaired normal function. Antioxidant compounds can counteract the damaging mechanisms associated with ROS. This study investigated the effect of (S)-Ketamine on reducing oxidative stress and its associated damage in a rat model of cerebral ischemia-reperfusion.
Methods: 28 male Wistar rats weighing 250-300 grams were randomly divided into four groups: control, ischemia-saline, ischemia-low dose esketamine, and ischemia-high dose esketamine. Ischemia was induced by occluding both common carotid arteries (CCA) for 20 minutes. Esketamine was injected immediately after ischemia at doses of 0.1 and 0.25 mg per kg body weight. The activity of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), was assessed using ELISA, while lipid peroxidation was evaluated using the thiobarbituric acid method.
Findings: MDA levels, as an indicator of oxidative stress, were higher in the CA1 region of the hippocampus in ischemic rats, while in the (S)-Ketamine-treated groups, the activities of antioxidant enzymes SOD, CAT, and GPx significantly increased and MDA levels decreased, indicating an increase in antioxidant capacity and a decrease in oxidative stress caused by ischemia-reperfusion in these groups.
Conclusion: (S)-Ketamine may play a crucial role in mitigating cerebral ischemia-reperfusion injury by reducing oxidative stress and enhancing antioxidant enzyme activity.

Highlights

Sohaila Erfani: Google Scholar

Bagher Amirheidari: Google Scholar

Seyedeh Mahdieh Khoshnazar: Google Scholar

Keywords

Main Subjects

References

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Journal of Isfahan Medical School
Volume 43, Issue 821
1st Week, September
September and October 2025
Pages 749-758

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History

  • Receive Date: 25 December 2024
  • Accept Date: 25 August 2025

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