Identification of a Common Immunogenic Epitope of IA-2 Protein Using Three Prediction Servers for the Design of Tolerogenic Vaccines in Type 1 Diabetes

Document Type : 6th congress of endocrinology & metabolism

Authors

1 Department of Medical Biotechnology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

2 Assistant Professor, Department of Medical Biotechnology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

3 Associate Professor, Department of Medical Genetics and Biotechnology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

4 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran

5 Department of medical biotechnology, Zanjan University of Medical Sciences, Zanjan, Iran

10.48305/jims.v43.i827.0980

Abstract

Background: Type 1 diabetes (T1D) is an autoimmune disease that destroys pancreatic β-cells, leading to less insulin production. Tolerogenic vaccines have emerged as a new approach to stop autoimmune reactions in T1D by inducing regulatory responses. Advanced bioinformatics tools like IEDB, TepiTool, and SYFPEITHI help in identifying effective epitopes for this purpose. In this study, identified the potential epitope APGFGSERGAPLAFA as promising vaccine candidate for T1D prevention or treatment.
Methods: The full amino acid sequence of the IA-2 protein was obtained from the UniProt database. IA-2, linked to type 1 diabetes, is a protein in pancreatic beta cells that acts as an autoantigen. To find Class II epitopes that could trigger an immune response in type 1 diabetes, several tools were used to predict how well peptide sequences from IA-2 bind to common MHC class II alleles. Key HLA alleles, specifically DRB1*01:01, DRB3*01:01, DRB4*01:01 and DRB5*01:01, which are associated with an elevated risk of type 1 diabetes, underwent analysis. Epitopes that received high scores were further examined to identify the most appropriate candidates for future diabetes treatments or diagnostic purposes.
Findings: The APGFGSERGAPLAFA epitope was identified as the sole common peptide exhibiting shared specificity across all three servers. Furthermore, it achieved high scores in SYFPEITHI and was placed among the top five peptides in TEPITOOL.
Conclusion: The simultaneous use of multiple epitope prediction tools can increase the accuracy in identifying immunological targets. However, further research is needed in this field.

Highlights

Hamed Esmaeil Lashgarian: Google Scholar, PubMed

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References

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Journal of Isfahan Medical School
Volume 43, Issue 827
3rd Week,, October: 6th International and 8th Iranian Congress of Endocrinology & Metabolism Updates
September and October 2025
Pages 980-982

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History

  • Receive Date: 05 June 2025
  • Accept Date: 10 June 2025

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