Prognostic Importance of Polymorphisms in DNA-Repair Gene (XRCC1 Arg399Gln) in Patients with Lung Cancer in Fars Province, Iran

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Cellular and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran

2 Assistant Professor, Department of Cell and Molecular Sciences, School of Biological Sciences, Kharazmi University, Tehran, Iran

3 Professor, Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Background: Genetic polymorphisms in DNA-repair genes may increase the risk of developing cancer due to reducing the DNA-repair capacity. XRCC1 is one of the important genes in DNA repair. This study was designed to examine the polymorphisms associated with XRCC1 Arg399Gln and to investigate its role as susceptibility marker for non-small cell lung cancer (NSCLC) in population of Fars province, Iran.Methods: In this case-control study, extracted DNA from 100 healthy controls and 100 patient of lung cancer were used to examine the role of XCRR1 Arg399Gln polymorphism in context of non-small cell lung cancer in population of Fars province in Iran. Amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used for determining of individuals’ genotyping.Findings: Our data showed a strong association between Gln/Gln (A/A) genotype and risk of developing non-small cell lung cancer in men. Moreover, men with at least one A allele (AA+ AG) showed reduced risk of developing non-small cell lung cancer. No such an associations were found in subgroups of women or when samples were divided based on their ages.Conclusion: According to our results, although there was no significant association between XRCC1 Age399Gln polymorphism and developing of lung cancer in population, men with Gln/Gln genotype were in high risk of developing non-small cell lung cancer in Fars province, Iran. Therefore, Gln/Gln polymorphism could be used as a biomarker for screening men at high risk of lung cancer.

Keywords

References

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Journal of Isfahan Medical School
Volume 34, Issue 403 - Serial Number 403
September and October 2016
Pages 1244-1250

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History

  • Receive Date: 20 August 2016
  • Revise Date: 30 May 2024
  • Accept Date: 06 April 2022

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