Document Type : Original Article(s)
Authors
1
Associate professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
2
Associate professor, Department of Cell Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
3
Associate professor, Deptartment of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
4
Assistant Professor, Department of gastroenterology, School of Medicine, Isfahan University of Medical Sciences
5
Associate professor, Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah
Abstract
BackgroundHelicobacter pylori (H. pylori) is a gram negative curved bacilli colonized in the human stomach. It causes duodenal ulcer, gastritis and is associated with adenocarinoma. There might be a possible relationship between cagA, vacA and ice a genes and clinical outcomes. The aim of this study was to identify the frequency of vacA genotypes of H. pylori isolated from the upper gastrointestinal. MethodsOne hundred H. pylori strains were isolated from patients with different gastrointestinal disease who admitted to Isfahan Al-zahra hospital. Vac A alleles were typed using PCR with specific primers. FindingsThere were four Vac A mosaicsms, including 28 for s1a/m1 (28%), 23 for s1b/m1 (23%), 26 for s1a/m2 (26%) and 23 for s1b/m2 (23%), s2 form was not found. ConclusionThe results showed there is no significant relationship between different genotypes of vacA and the related diseases. Key words: Helicobacter pylori, vac A, Adenocarinoma, Duodenal ulcer, Normal /Gastritis, PCR
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