Evaluation of IL-17 Gene Expression in the Peripheral Blood of Patients with Common Variable Immunodeficiency

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Immunology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

2 Assistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Infectious Diseases and Tropical Medicine Research Center AND Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

4 Associate Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 Associate Professor, Department of Biostatistics and Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Producing IL-17, Th17 cells induce BAFF and APRIL, two important mediators for B-cell survival and activation. Common variable immunodeficiency (CVID) is a group of many primary immunodeficiency diseases with a common set of features (including hypo-gammaglobulinemia) and different underlying causes. The prevalence of CVID is 1 to 50,000. Hence, the aim of this study was to measure IL-17 gene expression in peripheral blood of patients with CVID.Methods: Total mRNA extracted from whole blood samples of 10 patients with CVID and 10 normal controls; then, IL-17 gene was measured using qRT-PCR.Findings: There was a slight decrease in transcript levels of IL-17 in patients with CVID, even though, it was not statistically significant (P = 0.195).Conclusion: We showed that IL-17 is decreased in patients with CVID and this could be an explanation for their humoral immunity response insufficiencies. Absence of statistical significance in this IL-17 reduction may be due to our sample size and increasing the number of samples may result in statistical significance of this in future studies.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 230 - Serial Number 230
January and February 2013
Pages 358-363

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History

  • Receive Date: 11 March 2013
  • Revise Date:
  • Accept Date: 06 April 2022

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