The Effect of Aerobic Exercises with and Without Incline on the Expression of Some Genes Related to Muscle Atrophy in Mice with Chronic Kidney Disease

Document Type : Original Article(s)

Authors

1 PhD Student, Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Associate Professor, Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

10.48305/jims.v43.i809.0292

Abstract

Background: Chronic kidney disease is a catabolic disease, wich leads to muscle atrophy. The purpose of the research was to compare the effect uphill incline and zero incline training on improving  of muscle atrophy and kidney function of C57bl6 female mice suffering in chronic kidney disease(CKD).
Methods: A total of 24 two-month-old female C57b16 mice weighing 25 ± 26.1grams, were randomly divided into four groups: healthy control, patient control, patient and aerobic exercise with zero incline, and patient and aerobic exercise with positive incline. To induce CKD, they received adenine powder at a concentration of 0.2% mg/kg as feed for four weeks. The training groups performed eight weeks of treadmill training (five sessions per week) whit one group exercising on a 20-degree incline and the other on a zero-degree incline. After the last training session, blood samples were collected to measure blood uric acid, urea and creatinine using the ELISA method. The expression levels of the Myod, Myogenin, Myf-5genes were measured using qPCR (Real-Time PCR) technique. For statistical analysis, a one-way ANOVA test and Tukey's post hoc test were employed, with a significance level set at P≤0.05.
Findings: The results showed that induction of CKD caused a significant increase in the amount of urea, uric acid and serum creatinine compared to the healthy control group (P < 0.05). On the other hand, there is a significant decrease in the values of urea, uric acid and serum creatinine in the training group (P < 0.05). The expression levels of Myod, Myogenin and Myf-5 increased significantly in the slope training group (P < 0.05).
Conclusion: Exercises performed on an incline controls muscle atrophy and improves urea, uric acid and creatinine levels in mice with chronic kidney disease. Furthermore, the effects of incline exercise were found to be superior to those of exercise without incline.

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References

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Journal of Isfahan Medical School
Volume 43, Issue 809
1st Week, June
May and June 2025
Pages 292-301

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History

  • Receive Date: 25 November 2024
  • Accept Date: 26 May 2025

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