The Effect of Aerobic Training and Melatonin Consumption on Gene Expression of Some Inflammatory Factors and Antioxidant Index in Liver Tissue of Male Rats Diabetes

Document Type : Original Article (s)

Authors

1 Department of Physical Education & Sport Sciences, Sh.C., Islamic Azad University, Shoushtar, Iran

2 Department of Sport Sciences, MaS.C., Islamic Azad University, MasjedSoleiman, Iran

3 Department of Sport Sciences, School of Education and Psychology, Shiraz university, Shiraz, Iran

Abstract

Background: Diabetes, as a systemic disease, can lead to tissue damage and decreased liver function by increasing reactive species. Therefore, the purpose of this research is to investigate the interactive effects of aerobic training and melatonin consumption on the gene expression of some inflammatory factors and antioxidant markers in the liver tissue of male Diabetic Rats.
Methods: In this experimental study, 30 eight-week-old Wistar rats were randomly selected and Diabetes was induced in 24 rats via a single injection of streptozotocin (55 mg/kg). Diabetic rats were divided into 4 groups including diabetes control (DC), diabetes melatonin (DMel), diabetes aerobic training (DEx), diabetes aerobic training+melatonin (DEx+Mel). A healthy control group (HC) was also considered. The aerobic training program consisted of 5 days a week, for 6 weeks. The data were analyzed using one-way analysis of variance and Tukey's post-hoc statistical tests in SPSS version 29 software, with significance set at P ≤ 0.05.
Findings: Aerobic training and melatonin intake resulted in a significant decrease in IL-18 (P = 0.001) and IL-1β
(P = 0.005) gene expression. On the other hand, it resulted in a significant increase in SOD, GPx, TAC values and a significant decrease in MDA values in the intervention groups (P = 0.001).
Conclusion: Results indicate enhanced antioxidant defense and reduced inflammatory factors. Based on the results of this study, it is suggested that aerobic training combined with melatonin intake is more beneficial than aerobic training and melatonin intake separately.

Highlights

Shirin Zilaei Bouri: PubMed , Google Scholar 

Farhad Daryanoosh: PubMed , Google Scholar

Keywords

Main Subjects

References

  1. Talamantes S, Lisjak M, Gilglioni EH, Llamoza-Torres CJ, Ramos-Molina B, Gurzov EN. Non-alcoholic fatty liver disease and diabetes mellitus as growing aetiologies of hepatocellular carcinoma. JHEP Rep 2023; 5(9): 100811.
  2. Sabag A, Way KL, Sultana RN, Keating SE, Gerofi JA, Chuter VH, et al. The effect of a novel low-volume aerobic exercise intervention on liver fat in type 2 diabetes: a randomized controlled trial. Diabetes Care 2020; 43(10): 2371-8.
  3. Qadri S, Yki-Järvinen H. Surveillance of the liver in type 2 diabetes: important but unfeasible? Diabetologia 2024; 67(6): 961-73.
  4. Yki-Järvinen H, Luukkonen PK, Hodson L, Moore JB. Dietary carbohydrates and fats in nonalcoholic fatty liver disease. Nat Rev Gastroenterol Hepatol 2021; 18(11): 770-86.
  5. Zhu L, Tong H, Ren C, Chen K, Luo S, Wang Q, et al. Inflammation unleashed: The role of pyroptosis in chronic liver diseases. International Immunopharmacol 2024; 141: 113006.
  6. Fu J, Wu H. Structural mechanisms of NLRP3 inflammasome assembly and activation. Annu Rev Immunol 2023; 41: 301-16.
  7. Yang W, Liu L, Wei Y, Fang C, Liu S, Zhou F, et al. Exercise suppresses NLRP3 inflammasome activation in mice with diet-induced NASH: a plausible role of adropin. Lab Invest 2021; 101(3): 369-80.
  8. Ding Y, Xu X. Effects of regular exercise on inflammasome activation-related inflammatory cytokine levels in older adults: A systematic review and meta-analysis. J Sports Sci 2021; 39(20): 2338-52.
  9. Yu Y, Chen D, Zhao Y, Zhu J, Dong X. Melatonin ameliorates hepatic steatosis by inhibiting NLRP3 inflammasome in db/db mice. Int J Immunopathol Pharmacol 2021; 35: 20587384211036819.
  10. Rodríguez-Negrete EV, Morales-González Á, Madrigal-Santillán EO, Sánchez-Reyes K, Álvarez-González I, Madrigal-Bujaidar E, et al. Phytochemicals and Their Usefulness in the Maintenance of Health. Plants (Basel) 2024; 13(4): 523.
  11. Muscolo A, Mariateresa O, Giulio T, Mariateresa R. Oxidative stress: the role of antioxidant phytochemicals in the prevention and treatment of diseases. Int J Mol Sci 2024; 25(6): 3264.
  12. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev 2017; 2017: 8416763.
  13. Abedi A, Ghobadi H, Sharghi A, Iranpour S, Fazlzadeh M, Aslani MR. Effect of saffron supplementation on oxidative stress markers (MDA, TAC, TOS, GPx, SOD, and pro-oxidant/antioxidant balance): An updated systematic review and meta-analysis of randomized placebo-controlled trials. Front Med (Lausanne) 2023; 10:
  14. Singh A, Kukreti R, Saso L, Kukreti S. Oxidative stress: a key modulator in neurodegenerative diseases. Molecules 2019; 24(8): 1583.
  15. Briggs ON, Brown H, Elechi-amadi K, Ezeiruaku F, Nduka N. Superoxide dismutase and glutathione peroxidase levels in patients with long standing type 2 diabetes in Port Harcourt, Rivers State, Nigeria. International Journal of Science and Research 2016; 5(3): 1282-8.
  16. Dworzański J, Strycharz-Dudziak M, Kliszczewska E, Kiełczykowska M, Dworzańska A, Drop B, et al. Glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity in patients with diabetes mellitus type 2 infected with Epstein-Barr virus. PLoS One 2020; 15(3): e0230374.
  17. Rong B, Wu Q, Sun C. Melatonin: a novel strategy for prevention of obesity and fat accumulation in peripheral organs through the improvements of circadian rhythms and antioxidative capacity. Melatonin Research 2020; 3(1): 58-76.
  18. Heo JI, Yoon DW, Yu JH, Kim NH, Yoo HJ, Seo JA, et al. Melatonin improves insulin resistance and hepatic steatosis through attenuation of alpha-2-HS-glycoprotein. J Pineal Res 2018; 65(2): e12493.
  19. Reiter RJ, Tan DX, Rosales-Corral S, Galano A, Zhou XJ, Xu B. Mitochondria: central organelles for melatonin′ s antioxidant and anti-aging actions. Molecules 2018; 23(2): 509.
  20. Powers SK, Deminice R, Ozdemir M, Yoshihara T, Bomkamp MP, Hyatt H. Exercise-induced oxidative stress: Friend or foe? J Sport Health Sci 2020; 9(5): 415-25.
  21. Kruk J, Aboul-Enein BH, Duchnik E. Exercise-induced oxidative stress and melatonin supplementation: current evidence. J Physiol Sci 2021; 71(1): 27.
  22. Pedersen BK, Saltin B. Exercise as medicine–evidence for prescribing exercise as therapy in 26 different chronic diseases. Scand J Med Sci Sports 2015; 25(Suppl 3): 1-72.
  23. Al-Mhanna SB, Rocha-Rodriguesc S, Mohamed M, Batrakoulis A, Aldhahi MI, Afolabi HA, et al. Effects of combined aerobic exercise and diet on cardiometabolic health in patients with obesity and type 2 diabetes: a systematic review and meta-analysis. BMC Sports Sci Med Rehabil 2023; 15(1): 165.
  24. Gilyana M, Batrakoulis A, Zisi V. Physical activity, body image, and Emotional Intelligence Differences in adults with overweight and obesity. Diseases 2023; 11(2): 71.
  25. Sabouri M, Hatami E, Pournemati P, Shabkhiz F. Inflammatory, antioxidant and glycemic status to different mode of high-intensity training in type 2 diabetes mellitus. Mol Biol Rep 2021; 48(6): 5291-304.
  26. Mirzaei S, Sherafati Moghadam M, Dejdar N, Abdi M. The effect of endurance training on the amount of proteins involved in the regulation of adipose tissue metabolism in type 2 diabetic rats [in Persian]. Iranian Journal of Diabetes and Metabolism 2022; 22(5): 321-30.
  27. Garcia D, Shaw RJ. AMPK: mechanisms of cellular energy sensing and restoration of metabolic balance. Mol Cell 2017; 66(6): 789-800.
  28. Chae C-H, Jung S-L, An S-H, Jung C-K, Nam S-N, Kim H-T. Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase activation in the soleus of diabetic rats. J Physiol Biochem 2011; 67(2): 235-41.
  29. Zangiabadi N, Sheibani V, Asadi-Shekaari M, Shabani M, Jafari M, Asadi AR, et al. Effects of melatonin in prevention of neuropathy in STZ-induced diabetic rats. Am J Pharmacol Toxicol 2011; 6(2): 59-67.
  30. de Sousa Fernandes MS, de Simões E Silva LDL, Kubrusly MS, de Arruda Lima TRL, Muller CR, Américo ALV, et al. Aerobic exercise training exerts beneficial effects upon oxidative metabolism and non-enzymatic antioxidant defense in the liver of leptin deficiency mice. Front Endocrinol (Lausanne) 2020; 11:
  31. Sun Y, Ding S. NLRP3 inflammasome in diabetic cardiomyopathy and exercise intervention. Int J Mol Sci 2021; 22(24): 13228.
  32. Zhang Y, Liu Y, Liu X, Yuan X, Xiang M, Liu J, et al. Exercise and Metformin Intervention Prevents Lipotoxicity-Induced Hepatocyte Apoptosis by Alleviating Oxidative and ER Stress and Activating the AMPK/Nrf2/HO-1 Signaling Pathway in db/db Mice. Oxid Med Cell Longev 2022; 2022: 2297268.
  33. Abdelbasset WK, Tantawy SA, Kamel DM, Alqahtani BA, Elnegamy TE, Soliman GS, et al. Effects of high-intensity interval and moderate-intensity continuous aerobic exercise on diabetic obese patients with nonalcoholic fatty liver disease: a comparative randomized controlled trial. Medicine (Baltimore) 2020; 99(10): e19471.
  34. Rahbarghazi A, Alamdari KA, Rahbarghazi R, Salehi-Pourmehr H. Co-administration of exercise training and melatonin on the function of diabetic heart tissue: a systematic review and meta-analysis of rodent models. Diabetol Metab Syndr 2023; 15(1): 67.
  35. Farjallah M, Graja A, Mahmoud L, Ghattassi K, Boudaya M, Driss T, et al. Effects of melatonin ingestion on physical performance and biochemical responses following exhaustive running exercise in soccer players. Biol Sport 2022; 39(2): 473-9.
  36. Abou-Elnour ES, Hanna GS, Amer GS, Salem HR, Abdel-Razek HA, Ewida SF. Effect of exercise and melatonin on fructose-induced hepatic dysfunction in a metabolic syndrome rat model. Menoufia Medical Journal 2017; 30(1): 286-96.
  37. Aldahr MHS, Abd El-Kader SM. Impact of exercise on renal function, oxidative stress, and systemic inflammation among patients with type 2 diabetic nephropathy. Afr Health Sci 2022; 22(3): 286-95.
  38. Kristensen JM, Lillelund C, Kjøbsted R, Birk JB, Andersen NR, Nybo L, et al. Metformin does not compromise energy status in human skeletal muscle at rest or during acute exercise: a randomised, crossover trial. Physiol Rep 2019; 7(23): e14307.
  39. Caturano A, D’Angelo M, Mormone A, Russo V, Mollica MP, Salvatore T, et al. Oxidative stress in type 2 diabetes: impacts from pathogenesis to lifestyle modifications. Curr Issues Mol Biol 2023; 45(8): 6651-66.
Journal of Isfahan Medical School
Volume 43, Issue 808
4th Week, May
May and June 2025
Pages 244-254

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History

  • Receive Date: 09 November 2024
  • Accept Date: 05 May 2025

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