Investigation of the Healing Effects of Hydroethanolic Extract of Heracleum Persicum on Full-thickness Incisional Cutaneous Wounds in Rats

Document Type : Original Article(s)

Authors

1 Graduated in Veterinary Medicine, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

2 Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

3 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

Abstract

Background: Wound healing is the body's natural repair process after injury. Due to its high antioxidant capacity and many effective components, this study aimed to assess the impact of topically applied Heracleum persicum extract on rat full-thickness incisional cutaneous wound healing.
Methods: After induction the wound, sixty male rats were divided into four groups: control, basal cream, H. persicum 5% and 10% and were treated daily with saline, basal cream, H. persicum 5% and H. persicum 10% for 14 days, respectively. The animals were euthanized at 7, 14 and 21 days post-injury, and samples were collected for histopathological and biochemical analysis.
Findings: A prominent decrease in the wound area was observed in the treatment groups, particularly H. persicum 10% compared to the control group. Additionally, treatment with H. persicum decreased the number of lymphocytes and improved the number of fibroblasts at the earlier stages and increased the number of fibrocytes at the later stages of wound healing. Also, re-epithelization, tissue alignment, higher maturity of the collagen fibers and large capillary-sized blood vessels revealed significant changes in the treatment groups, especially H. persicum 10% compared to the control group. H. persicum extracts increased hydroxyproline and glycosaminoglycans contents and elevated total antioxidant capacity, while decreased malondialdehyde compared to the control group.
Conclusion: This study showed that H. persicum promotes wound healing activity. This property can be attributed to its action on blood vessels, modulation of inflammation, its role in initiating more collagen and glycosaminoglycan production and decreasing oxidative stress.

Highlights

Fatemeh Hoseinpour: Google Scholar 

Mohammad Hashemnia: Google Scholar 

Keywords

Main Subjects

References

  1. Borena BM, Martens A, Broeckx SY, Meyer E, Chiers K, Duchateau L, et al. Regenerative Skin wound healing in mammals: State-of-the-art on growth factor and stem cell based treatments. Cell Physiol Biochem 2015; 36(1): 1-23.
  2. Tavakoli A, Vazirinejad R, Ansari Jaberi A, Negahban T, Mashayekhi H, Nazari M, et al. Effect of Teucrium polium extract on skin wound healing in rat [in Persian]. Hormozgan Medical Journal 2012; 16(1): 17-24.
  3. Wang G, Yang F, Zhou W, Xiao N, Luo M, Tang Z. The initiation of oxidative stress and therapeutic strategies in wound healing. Biomed Pharmacother 2023; 157: 114004.
  4. Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Abdull Razis AF, Modu B, et al. Oxidative stress, free radicals and antioxidants: Potential crosstalk in the pathophysiology of human diseases. Front Chem 2023; 11: 1158198.
  5. de Oliveira Gonzalez AC, Costa TF, de Araújo Andrade Z, Medrado AR. Wound healing-A literature review. An Bras Dermatol 2016; 91(5): 614-20.
  6. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound healing: a cellular perspective. Physiol Rev 2019; 99(1): 665-706.
  7. Manou D, Caon I, Bouris P, Triantaphyllidou IE, Giaroni C, Passi A, et al. The complex interplay between extracellular matrix and cells in tissues. Methods Mol Biol 2019; 1952: 1-20.
  8. Cramer MC, Badylak SF. Extracellular matrix-based biomaterials and their influence upon cell behavior. Ann Biomed Eng 2020; 48(7): 2132-53.
  9. Xue M, Jackson CJ. Extracellular Matrix Reorganization During Wound Healing and Its Impact on Abnormal Scarring. Adv Wound Care (New Rochelle) 2015; 4(3): 119–36.
  10. Bayer IS. Hyaluronic acid and controlled release: a review. Molecules 2020; 25(11): 2649.
  11. Atiyeh BS, Gunn SW, Hayek SN. State of the art in burn treatment. World J Surg 2005; 29(2): 131-48.
  12. Vitale S, Colanero S, Placidi M, Di Emidio G, Tatone C, Amicarelli F, et al. Phytochemistry and biological activity of medicinal plants in wound healing: an overview of current research. Molecules 2022; 27(11): 3566.
  13. Farzaei MH, Khanavi M, Moghaddam G, Dolatshahi F, Rahimi R, Shams-Ardekani MR, et al. Standardization of Tragopogon graminifolius DC. extract based on phenolic compounds and antioxidant activity. J Chem 2014; 2014(1): 425965.
  14. Hanachi P, Gharari Z, Sadeghinia H, Walker TR. Synthesis of bioactive silver nanoparticles with eco-friendly processes using Heracleum persicum stem extract and evaluation of their antioxidant, antibacterial, anticancer and apoptotic potential. J Mol Struct 2022; 1265: 133325.
  15. Hemati A, Azarnia M, Angaji SA. Medicinal effects of Heracleum persicum (Golpar). Middle East J Sci 2010; 5(3): 174-6.
  16. Asgarpanah J, Dadashzadeh Mehrabani G, Ahmadi M, Ranjbar R, Safi-Aldin Ardebily M. Chemistry, pharmacology and medicinal properties of Heracleum persicum Desf. Ex Fischer: A review. J Med Plants Res 2012; 6(10): 1813-20.
  17. Majidi Z, Sadati Lamardi SN. Phytochemistry and biological activities of Heracleum persicum: A review. J Integr Med 2018; 16(4): 223-35.
  18. Hemmati AA, Rashidi I, Dahanzadeh S, Moeini M. Investigating the healing effect of the hydroalcoholic extract of pomegranate seed (Punica granatum) on the full thickness wound in rabbit. J Contemp Med Sci 2020; 6(2): 66-72.
  19. Tekleyes B, Huluka SA, Wondu K, Wondmkun YT. Wound healing activity of 80% methanol leaf extract of Zehneria scabra (Lf) Sond (Cucurbitaceae) in mice. J Exp Pharmacol 2021; 31: 537-44.
  20. Brand-Williams W, Cuvelier ME, Berset CL. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 1995; 28(1): 25-30.
  21. Lamuela‐Raventós RM. Folin–Ciocalteu method for the measurement of total phenolic content and antioxidant capacity. In: Apak R, Capanoglu E, Shahidi F, editors. Measurement of antioxidant activity & capacity: recent trends and applications. 1st ed. New York, NY: John Wiley & Sons Ltd; 2018. p. 107-15.
  22. Nugroho AE, Malik A, Pramono S. Total phenolic and flavonoid contents, and in vitro antihypertension activity of purified extract of Indonesian cashew leaves (Anacardium occidentale L.). Int Food Res J 2013; 20(1): 299-305.
  23. Hoseinpour F, Hashemnia M, Cheraghi H, Safikhani R, Sheikhi T, Hasani Z. Assessment of antioxidant and cutaneous wound healing effects of Ornithogalum cuspidatum hydroalcoholic extract in male Wistar rats. J Basic Clin Pathophysiol 2021; 9(1): 23-32.
  24. Derakhshanfar A, Kian M, Dehghan Z, Valizadeh A, Moayedi J. Comparison of the effects of two methods of euthanasia on post mortem changes in rats: histopathological and molecular findings. Comp Clin Path 2022; 31(5): 815-26.
  25. Oryan A, Mohammadalipour A, Moshiri A, Tabandeh MR. Avocado/soybean unsaponifiables: a novel regulator of cutaneous wound healing, modelling and remodelling. Int Wound J 2015; 12(6): 674-85.
  26. Goudarzi F, Mohammadalipour A, Bahabadi M, Goodarzi MT, Sarveazad A, Khodadadi I. Hydrogen peroxide: a potent inducer of differentiation of human adipose-derived stem cells into chondrocytes. Free Radic Res 2018; 52(7): 763-74.
  27. Hashemnia M, Nikousefat Z, Mohammadalipour A, Zangeneh MM, Zangeneh A. Wound healing activity of Pimpinella anisum methanolic extract in streptozotocin-induced diabetic rats. J Wound Care 2019; 28(10): 26-36.
  28. Hajhashemi V, Sajjadi SE, Heshmati M. Anti-inflammatory and analgesic properties of Heracleum persicum essential oil and hydroalcoholic extract in

    animal models. J Ethnopharmacol 2009; 124(3): 475-80.
  29. Sharififar F, Pournourmohammadi S, Arabnejad M, Rastegarianzadeh R, Ranjbaran O, Purhemmaty A. Immunomodulatory activity of aqueous extract of heracleum persicum Desf. in Mice. Iran J Pharm Res 2009; 8(4): 287-92.
  30. Kumari M, Eesha BR, Amberkar M, Kumar N. Wound healing activity of aqueous extract of Crotalaria verrucosa in Wistar albino rats. Asian Pac J Trop Med 2010; 3(10): 783-7.
  31. Courtois MC, Sapoval M, Del Giudice C, Ducloux R, Mirault T, Messas E. Distal revascularization in diabetic patients with chronic limb ischemia. J Mal Vasc 2015; 40(1): 24-36.
  32. Oryan A, Naeini AT, Moshiri A, Mohammadalipour A, Tabandeh MR. Modulation of cutaneous wound healing by silymarin in rats. J Wound Care 2012; 21(9): 457-64.
  33. Yang LL, Liang YC, Chang CW, Lee WS, Kuo CT, Wang CC, et al. Effects of sphondin, isolated from Heracleum laciniatum, on IL–1β-induced cyclooxygenase-2 expression in human pulmonary epithelial cells. Life Sci 2002; 72(2): 199-213.
  34. Caetano GF, Fronza M, Leite MN, Gomes A, Frade MA. Comparison of collagen content in skin wounds evaluated by biochemical assay and by computer-aided histomorphometric analysis. Pharm Biol 2016; 54(11): 2555-9.
  35. Lorente L, Rodriguez ST, Sanz P, Abreu-González P, Díaz D, Moreno AM, et al. Association between pre-transplant serum malondialdehyde levels and survival one year after liver transplantation for hepatocellular carcinoma. Int J Mol Sci 2016; 17(4): 500.
  36. Zhang B, Zehnder JL. Oxidative stress and immune thrombocytopenia. Semin Hematol 2013; 50(3): e1-e4.
  37. Dunnill C, Patton T, Brennan J, Barrett J, Dryden M, Cooke J, et al. Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS‐modulating technologies for augmentation of the healing process. Int Wound J 2017; 14(1): 89-96.
  38. Merecz-Sadowska A, Sitarek P, Kucharska E, Kowalczyk T, Zajdel K, Cegliński T, et al. Antioxidant properties of plant-derived phenolic compounds and their effect on skin fibroblast cells. Antioxidants (Basel) 2021; 10(5): 726.
  39. Akbaribazm M, Goodarzi N, Rahimi M, Naseri L, Khazaei M. Anti-inflammatory, anti-oxidative and anti-apoptotic effects of Heracleum persicum L. extract on rats with gentamicin-induced nephrotoxicity. Asian Pac J Trop Biomed 2021; 11(2): 47-58.
  40. Kant V, Sharma M, Jangir BL, Kumar V. Acceleration of wound healing by quercetin in diabetic rats requires mitigation of oxidative stress and stimulation of the proliferative phase. Biotech Histochem 2022; 97(6): 461-72.
  41. Ma X, Lin Y, Liu Y, Li W, He J, Fang M, et al. Effects of apigenin treatment on random skin flap survival in rats. Front Pharmacol 2021; 12: 625733.
  42. Al-Numair KS, Chandramohan G, Veeramani C, Alsaif MA. Ameliorative effect of kaempferol, a flavonoid, on oxidative stress in streptozotocin-induced diabetic rats. Redox Rep 2015; 20(5): 198-209.
Journal of Isfahan Medical School
Volume 43, Issue 802
2nd Week, April
March and April 2025
Pages 30-45

Files

History

  • Receive Date: 17 October 2024
  • Accept Date: 16 February 2025

Share

How to cite

Statistics