Journal of Isfahan Medical School

Journal of Isfahan Medical School

Comparing Disk Diffusion and E-Test Methods in Reviewing Methicillin-Resistance in Staphylococcus Epidermidis Isolated from Clinical Speci-mens

Document Type : Original Article (s)

Authors
Ebtehaj Pishva 1
Sayed Asghar Havaei 1
Firooz Arsalani 2
1 Associate Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Student of Medicine, School of Medicine and Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: In recent years, antibiotic resistance of staphylococcus epidermidis toward methicillin has been a significant increase. Therefore, reviewing methicillin-resistance is necessary and crucial in determining an appropriate therapeutic pattern. This study aimed to compare disk fusion and E-test methods in reviewing methicillin-resistance in staphylococcus epidermidis isolated from clinical specimens.Methods: Determination of methicillin resistance was conducted through disk fusion and E-test methods on 146 samples of staphylococcus epidermidis and these two methods were compared to each other.Findings: 146 samples of staphylococcus epidermidis were evaluated through disk fusion and E-test methods in terms of methicillin resistance in which the resistant rate of E-test was 70.54 percent and disk fusion was 61.64 percent and their sensitivity and specificity in E-test were 95.3 and 94.7 percent respectively and in disk fusion were 86.5 and 80.9 percent respectively.Conclusion: Assessing the sensitivity and specificity in E-test and disk fusion methods showed that E-test had a higher sensitivity and specificity and also is a faster, more accurate and more reliable method to determine methicillin resistance in staphylococcus epidermidis.
Keywords
Staphylococcus epidermidis
Methicillin
E-Test
Disk fusion
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Journal of Isfahan Medical School
Volume 29, Issue 156 - Serial Number 156
July and August 2011
Pages 1493-1500

  • Receive Date 23 October 2011
  • Accept Date 06 April 2022