Journal of Isfahan Medical School

Journal of Isfahan Medical School

A Comparative Survey on the Expression of P16 as a Tumor Suppressor Protein in Cancerous and Non-Cancerous Colorectal Tissue Samples Using Immunohistochemistry Method

Document Type : Original Article (s)

Authors
Mehdi Nikbakht Dastjerdi 1
Mohammad Moeini 2
1 Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Student of Medicine, School of Medicine And Student Research committee, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: P16 is known as a tumor suppressor protein. This study was conducted to evaluate the association of P16 expression and pathogenesis of colorectal cancer. The presence of P16 in cancerous and non-cancerous colorectal tissue samples were thus compared using immunohistochemistry method. Methods: In this stud, 50 paired tissue samples were provided. The pairs were comprised of samples of cancerous colorectal tissue and normal peers from non-cancerous tissue adjacent to cancerous ones. Expression of P16 was studied on paraffined sections using immunohistochemistry method. Findings: Although P16 was expressed in all tissue samples, we found a lower expression in colorectal cancerous tissue in comparison with normal tissue samples. This difference was revealed to be statistically significant (P < 0.001). Conclusion: The presence of P16 can be regarded as an appropriate biological marker and a valuable prognostic factor for colorectal cancer. Keywords: Colorectal cancer, Immunohistochemistry, Tumor suppressor gene, P16INK4a

  • Receive Date 16 June 2012
  • Accept Date 06 April 2022