Journal of Isfahan Medical School

Journal of Isfahan Medical School

Effect of Acute Resistance Training on Plasma Irisin Protein Level and Expression of Muscle FNDC5 and Adipose Tissue UCP1 Genes in Male Rats

Document Type : Original Article (s)

Authors
Jalil Reisi 1
Hamid Rajabi 2
Kamran Ghaedi 3
Seyed-Mohammad Marandi 4
Mohammad-Reza Dehkhoda 2
1 PhD Student, Department of Exercise Physiology, School of Physical Education, Kharazmi University, Tehran, Iran
2 Associate Professor, Department of Exercise Physiology, School of Physical Education, Kharazmi University, Tehran, Iran
3 Associate Professor, Department of Biology, School of Science, University of Isfahan, Isfahan, Iran
4 Associate Professor, Department of Exercise Physiology, School of Physical Education, University of Isfahan, Isfahan, Iran
Abstract
Background: Change in adipose tissue phenotype caused by exercise training is the new theory raised recently. However, the identification of cellular and molecular mechanisms by which exercise training exert its benefits are being investigated. The purpose of this study was to determine the effect of resistance training on plasma irisin protein level and expression of soleus muscle FNDC5 and subcutaneous adipose tissue UCP1 genes in male rats.Methods: 16 Sprague dolly male rats (mean weight = 221.00 ± 2.31 g; age: 8 weeks) divided in 2 groups, resistance training and control, randomly. Training group exercised on a special ladder (1.2 meters height) and carried a weight with 50% of their body weight, which was closed to their tails, 3 sets of 5 reps with 3 minutes rest between training sessions and one minute rest between repetitions was done. The enzyme-linked immunosorbent assay (ELISA) method for measuring plasma irisin levels and real-time polymerase chain reaction (real-time PCR) method for the relative expression of mRNA of UCP1 and FNDC5 genes were used. Independent t-test was used to compare group differences.Findings: Irisin protein levels after resistance training significantly increased (P < 0.001). Besides, the relative expression of mRNA of FNDC5 (P < 0.001) and UCP1 (P < 0.001) genes significantly increased after exercise.Conclusion: Although research on this theory is a beginning pathway, it can be concluded from the present results that the resistance training through secretion of myokin-like irisin improves body composition possibly through increased energy expenditure in white adipose tissue.
Keywords
Acute resistance training
Irisin
UCP1 gene
FNDC5 gene
  1. Dunstan D. Diabetes: Exercise and T2DM[mdash]move muscles more often! Nat Rev Endocrinol 2011; 7(4): 189-90.
  2. Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012; 481(7382): 463-8.
  3. Handschin C, Spiegelman BM. The role of exercise and PGC1alpha in inflammation and chronic disease. Nature 2008; 454(7203): 463-9.
  4. van Marken Lichtenbelt WD, Vanhommerig JW, Smulders NM, Drossaerts JM, Kemerink GJ, Bouvy ND, et al. Cold-activated brown adipose tissue in healthy men. N Engl J Med 2009; 360(15): 1500-8.
  5. Wenz T, Rossi SG, Rotundo RL, Spiegelman BM, Moraes CT. Increased muscle PGC-1alpha expression protects from sarcopenia and metabolic disease during aging. Proc Natl Acad Sci U S A 2009; 106(48): 20405-10.
  6. Xu X, Ying Z, Cai M, Xu Z, Li Y, Jiang SY, et al. Exercise ameliorates high-fat diet-induced metabolic and vascular dysfunction, and increases adipocyte progenitor cell population in brown adipose tissue. Am J Physiol Regul Integr Comp Physiol 2011; 300(5): R1115-R1125.
  7. Blair SN, LaMonte MJ, Nichaman MZ. The evolution of physical activity recommendations: how much is enough? Am J Clin Nutr 2004; 79(5): 913S-20S.
  8. Kelley GA, Kelley KS. Impact of progressive resistance training on lipids and lipoproteins in adults: a meta-analysis of randomized controlled trials. Prev Med 2009; 48(1): 9-19.
  9. Ormsbee MJ, Thyfault JP, Johnson EA, Kraus RM, Choi MD, Hickner RC. Fat metabolism and acute resistance exercise in trained men. J Appl Physiol (1985 ) 2007; 102(5): 1767-72.
  10. Pitsavos C, Panagiotakos DB, Tambalis KD, Chrysohoou C, Sidossis LS, Skoumas J, et al. Resistance exercise plus to aerobic activities is associated with better lipids' profile among healthy individuals: the ATTICA study. QJM 2009; 102(9): 609-16.
  11. Volek JS, Kraemer WJ, Bush JA, Boetes M, Incledon T, Clark KL, et al. Creatine supplementation enhances muscular performance during high-intensity resistance exercise. J Am Diet Assoc 1997; 97(7): 765-70.
  12. Winnick JJ, Gaillard T, Schuster DP. Resistance training differentially affects weight loss and glucose metabolism of White and African American patients with type 2 diabetes mellitus. Ethn Dis 2008; 18(2): 152-6.
  13. Saghebjoo M, Dastigerdi S, Afzalpour ME, Hedayati M. Effects of aerobic and resistance training on plasma visfatin levels in overweight women. Koomesh 2012; 13(2): 225-32. [In Persian].
  14. Lee S, Farrar RP. Resistance training induces muscle-specific changes in muscle mass and function in rat. J Exercise Physiol Online 2003; 6(2): 80-7.
  15. MacIntosh B, Gardiner P, McComas A. Skeletal muscle: form and function. Trans. Gharakhanlo R, Azad A, Gorzi A. Tehran, Iran: Samt Publication; 2011. [In Persian].
  16. Timmons JA, Baar K, Davidsen PK, Atherton PJ. Is irisin a human exercise gene? Nature 2012; 488(7413): E9-10.
  17. Pedersen BK. A muscular twist on the fate of fat. N Engl J Med 2012; 366(16): 1544-5.
  18. Moon HS, Dincer F, Mantzoros CS. Pharmacological concentrations of irisin increase cell proliferation without influencing markers of neurite outgrowth and synaptogenesis in mouse H19-7 hippocampal cell lines. Metabolism 2013; 62(8): 1131-6.
  19. Moon HS, Dincer F, Mantzoros CS. Amylin-induced downregulation of hippocampal neurogenesis is attenuated by leptin in a STAT3/AMPK/ERK-dependent manner in mice. Diabetologia 2013; 56(3): 627-34.
Journal of Isfahan Medical School
Volume 31, Issue 256 - Serial Number 256
September and October 2013
Pages 1657-1666

  • Receive Date 08 July 2013
  • Accept Date 06 April 2022