Journal of Isfahan Medical School

Journal of Isfahan Medical School

Effects of Biosurfactants Derived from Lactobacillus Casei and Lactobacillus Reuteri on Gene Expression Profile of gtfB/C and ftf Genes in Lactobacillus Mutans

Document Type : Original Article (s)

Authors
Omid Savabi 1
Mohammad Kazemi 2
Rasoul Salehi 3
1 Associate Professor, Department of Dental Prosthetics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
2 Pediatric Inherited Diseases Research Center AND Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Pediatric Inherited Diseases Research Center AND Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Dental caries is a worldwide health problem involving almost all age groups in underdeveloped as well as developed countries. So for no considerable progress achieved in the disease prevention, so new preventive/thrapeutique measures are urgently needed. Probiotic bacteria and their biproducts, like biosurfactants, are cost-effective with no considerable side effects for dental caries prevention. The aim of the present study was to evaluate the effects of biosurfactants purified from Lactobacillus casei (ATCC39392) and Lactobacillus reuteri (DSM20016) on ftf and gtf B/C gene expression level of the standard strains of streptococcus mutans (ATCC35668).Methods: Lactobacillus species purchased from the bacterial collection center of Iran. For biofilm production, microtiter plate assay in presence of 1% glucose was used. For biosurfactant production, lactobacillus species were cultured in MRS broth medium, dialyzed and lyophilized using freeze-dryer. To evaluate the effects of biosurfactant on gene expression, bacterial cells were cultured in presence of the appropriate biosurfactant (2.5 mg/ml); after four hours incubation, total cellular RNA was then extracted and used for cDNA synthesis.Findings: Expression of ftf and gtf B/C genes of the standard strains of Streptococcus mutans (ATCC35668) was considerably down regulated by the application of the biosurfactants. The reduction in gene expression was statistically significant (P < 0.05).Conclusion: Considerable down regulation of all genes in presence of biosurfactants was observed. Therefore, it is concluded that the biosurfactants prepared in this study is very successful in dental caries prevention.
Keywords
gtfB/C gene
ftf gene
Biosurfactant
Real time quantitative polymerase chain reaction
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Journal of Isfahan Medical School
Volume 31, Issue 260 - Serial Number 260
September and October 2014
Pages 1829-1835

  • Receive Date 24 October 2013
  • Accept Date 06 April 2022