Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of Follicle Stimulating Hormone (FSH) on Colony Formation of Ovine Spermatogonial Stem Cells in Vitro

Document Type : Original Article (s)

Authors
Parviz Tajik 1
Mohammad-Reza Mokhber-Dezfuli 1
Nafise Alighazi 2
Peyman Rahimi-Feyli 3
Shiva Shafiei 4
1 Professor, Department of Clinical Sciences, School of Veterinary Medicine, University of Tehran, Tehran, Iran
2 Veterinarian, School of Veterinary Medicine, University of Tehran, Tehran, Iran
3 Assistant Professor, Department of Clinical Sciences, School of Veterinary Medicine, Razi University, Kermanshah, Iran
4 School of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract
Background: Spermatogonial stem cells (SSCs) are undifferentiated cells with special capabilities on the self-renewing and differentiation. Proliferation of SSCs is a primary requirement for the study of their characteristics and function in vitro. The aim of this study was to evaluate the effects of different concentrations of follicle stimulating hormone (FSH) on the colonization activity of ovine SSCs in a short-term in-vitro co-culture with sertoli cells.Methods: Both sertoli and spermatogonial cells were isolated from 2-3 months old lamb testes by two-steps enzymatic digestion. Afterward, isolated cells were cultured in four groups with different concentrations of FSH (0, 5, 10 and 15 IU/ml, respectively) for 10 days. Colony assay (number and surface area) was evaluated 4, 7 and 10 days after the beginning of the culture by light microscope.Findings: At the day 4, colony surfaces of groups 1 and 2 were significantly more than groups 3 and 4 (P < 0.05). At the days 7 and 10, colony surfaces of groups 1 and 2 were significantly more than group 4 (P < 0.05).Conclusion: The results showed that treated groups with 10 and 15 IU/ml of FSH significantly decreased colony surface of ovine SSCs in comparison with co-culture system.
Keywords
Spermatogonia
Stem cell
Sertoli cell
Follicle stimulating hormone (FSH)
Ovine
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Journal of Isfahan Medical School
Volume 31, Issue 268 - Serial Number 268
November and December 2014
Pages 2228-2237

  • Receive Date 11 June 2013
  • Accept Date 06 April 2022