Journal of Isfahan Medical School

Journal of Isfahan Medical School

Evaluation of the Frequency of Osteopontin Gene Polymorphism (rs28357094 T>G) and its Receptor Gene (ITGA4- rs1449263 A>G) in Multiple Sclerosis Patients in Comparison to Healthy Controls in Isfahan, Iran, Population

Document Type : Original Article (s)

Authors
Fahimeh Dadkhah 1
Fereshteh Alsahebfosoul 2
Nahid Eskandari 2
Mansour Salehi 3
Masoud Etemadifar 4
Mohammad Kazemi 5
1 MSc Student, Department of Immunology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department of Cellular and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Professor, Department of Neurosciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
5 PhD Student, Department of Cellular and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS). Various factors including genetic and environmental factors are involved in the prevalence of MS. The Osteopontin (OPN) and its receptor Integrins alpha-4/beta-1 (ITGA4) have important role in immune response, so their encoding gene could be considered as a candidate for susceptibility to MS diseases. In the present study, we evaluated the frequency of OPN (rs28357094T > G) and ITGA4 (rs1449263A>G) polymorphisms in MS patients compared with healthy controls in Isfahan, Iran population.Methods: Blood samples were collected from 100 patients with multiple sclerosis and 100 healthy controls. After DNA extraction, OPN (rs28357094) and ITGA4(rs1449263) polymorphisms were detected with high resolution melt real time polymerase chain reaction (HRM Real Time PCR) technique. The results were analyzed with the SPSS software.Findings: In this study, frequency of TT genotype (OPN-rs28357094) (P = 0.020, OR = 1.85) and AA genotype (ITGA4 - rs1449263) (P = 0.012, OR = 2.10) in Patients with multiple sclerosis were higher than the control group significantly.Conclusion: The results of this study show that rs28357094 T>G on OPN gene and ITGA4-rs1449263A > G polymorphisms were associated with susceptibility to multiple sclerosis in Isfahan population.
Keywords
Multiple Sclerosis
Polymorphism
OPN gene
ITGA4 gene
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Journal of Isfahan Medical School
Volume 34, Issue 380 - Serial Number 380
March and April 2016
Pages 422-429

  • Receive Date 26 October 2015
  • Accept Date 06 April 2022