اثر حفاظتی 6 هفته تمرین اختیاری بر آپوپتوز کبدی ناشی از دوکسوروبیسین در Ratهای مدل سالمندی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزش، گروه تربیت بدنی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران

2 استاد، گروه فیزیولوژی ورزش، دانشکده‌ی علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

3 استادیار، گروه پزشکی مولکولی، دانشکده‌ی فن‌آوری‌های نوین در علوم پزشکی و مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی گلستان، گرگان، ایران

چکیده

مقدمه: روش‌های تمرینی مختلفی جهت کاهش عوارض داروی دوکسوروبیسین ارایه شده‌اند که به نظر می‌رسد با توجه به شرایط بیماران، زمان شروع تمرینات، طول دوره و استرس تمرینی باید مورد توجه بیشتری قرار بگیرند. از این رو، هدف از انجام این مطالعه، بررسی اثر حفاظتی تمرین اختیاری بر آپوپتوز کبدی ناشی از دوکسوروبیسین در Ratهای مدل سالمندی بود.روش‌ها: 42 سر Rat بالغ نژاد Wistar، در 6 گروه (7 = n) شامل جوان، سالمند، سالمند + سالین، سالمند + دوکسوروبیسین، سالمند + دوکسوروبیسین + تمرین اختیاری و سالمند + سالین + تمرین اختیاری قرار گرفتند. تمرین اختیاری، در قفس‌های مجهز به چرخ دوار و شمارشگر مسافت، به مدت 6 هفته به صورت انفرادی و آزادانه انجام شد. هم‌زمان با 15 روز پایانی تمرین، دوکسوروبیسین یا محلول سالین با دز روزانه‌ی 1 میلی‌گرم/کیلوگرم (دز تجمعی 15 میلی‌گرم/کیلوگرم) تزریق شد. 48 ساعت پس از اتمام تمرین، بافت‌برداری از کبد برای ارزیابی بیان پروتئین‌های Bax و B-cell leukemia 2 (Bcl-2) جهت بررسی آپوپتوز به روش Western blot انجام شد.یافته‌ها: آزمون One-way ANOVA نشان داد در گروه‌های تمرینی نسبت به سایر گروه‌ها، میزان بیان پروتئین Bax و نسبت Bcl-2/Bax کاهش معنی‌دار و بیان پروتئین Bcl-2، افزایش معنی‌داری یافته بود.نتیجه‌گیری: تمرین اختیاری قبل و حین شیمی‌درمانی، ممکن است بتواند از طریق کاهش پروتئین Bax و نسبت Bcl-2/Bax، باعث کاهش آپوپتوز و افزایش بقا در هپاتوسیت‌های سالم سالمندان و در نتیجه، کاهش عوارض دوکسوروبیسین شود.

کلیدواژه‌ها

عنوان مقاله [English]

The Protective Effect of 6 Weeks of Voluntary Training on Liver Apoptosis Induced by Doxorubicin in Aging Model Rats

نویسندگان [English]

  • Minoo Dadban-Shahamat 1
  • Valiollah Dabidi-Roshan 2
  • Touraj Farazmandfar 3
1 PhD Student in Exercise Physiology, Department of Physical Education, Sari Branch, Islamic Azad University, Sari, Iran
2 Professor, Department of Exercise Physiology, School of Sport Sciences, University of Mazandaran, Babolsar, Iran
3 Assistant Professor, Department of Molecular Medicine, School of Advanced Medical Technologies AND Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
چکیده [English]

Background: Different training methods have been presented to reduce the side effects of doxorubicin and it seems that according to patient's condition, the time to start exercises, and the duration and stress of exercise should be considered. This study aimed to investigate the preventive effect of voluntary exercise on liver apoptosis induced by doxorubicin in aging model rats.Methods: 42 adult male Wistar rats were divided into 6 equal groups of young, aging, aging + saline, aging + doxorubicin, aging + doxorubicin + voluntary training, and aging + saline+ voluntary training. Voluntary training was performed in cages equipped with a running wheel for six weeks individually and freely. At the same time, as the end of the training, doxorubicin or saline solution was injected with a cumulative dose (1 mg/kg). 48 hours after completion of the training, liver tissue sampling was performed to assess the apoptosis via examining the expression of Bax and B-cell leukemia 2 (Bcl-2) proteins using western blot method.Findings: One-way analysis of variance showed that in the training groups, the expression level of Bax protein and Bax/Bcl-2 ratio decreased significantly, and the expression of Bcl-2 protein was significantly increased in comparison to the control groups.Conclusion: Voluntary exercises prior to treatment with doxorubicin can reduce apoptosis and increase survival in healthy elderly hepatocytes via reducing the Bax protein and the Bax/Bcl-2 ratio, thereby reducing the side effects of doxorubicin.

کلیدواژه‌ها [English]

  • Exercise
  • Apoptosis
  • Doxorubicin
  • Aging, Bax protein
  • B-cell leukemia 2 family proteins

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مجله دانشکده پزشکی اصفهان
دوره 36، شماره 465 - شماره پیاپی 465
فروردین و اردیبهشت 1397
صفحه 14-21

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سابقه مقاله

  • تاریخ دریافت: 06 دی 1396
  • تاریخ بازنگری: 31 فروردین 1403
  • تاریخ پذیرش: 17 فروردین 1401

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