Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of Long Non-Coding RNA PVT1 Inhibition by Antisense LNA GapmeRs Technology on Proliferation of Human Acute Erythroleukemia Cells

Document Type : Original Article (s)

Authors
Mahsa Salehi 1
Mohammadreza Sharifi 2
1 MSc Student, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Associate Professor, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
10.22122/jims.v36i477.9946
Abstract
Background: Long non-coding RNAs (LncRNAs) are a class of RNA molecules with 200-10000 nucleotides in length that have lost their protein coding capacity. They play important role in numerous biological processes including cell proliferation, differentiation, apoptosis, and cancer development. Acute myeloid leukemia (AML) is the most common leukemia. Evidence has shown that long non-coding RNAs PVT1 is upregulated in acute myeloid leukemia, and leads to an increase in cell proliferation. This study aimed to assess the effect of long non-coding RNA PVT1 inhibition by antisense LNA GapmeRs technology on proliferation of human acute erythroleukemia cells.Methods: Human acute erythroleukemia cells (KG1), known as subgroup six of acute myeloid leukemia based on the French–American–British (FAB) classification, were cultured in Roswell Park Memorial Institute (RPMI) medium. Long non-coding RNA PVT1 degradation was performed using antisense LNA GapmeRs technology. At different time points after transfection (24, 48, and 72 hours), long non-coding RNA PVT1 expression and cell proliferation were assessed. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was accomplished to evaluate the long non-coding RNAPVT1 expression. Moreover, Cell viability was measured using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) assay.Findings: Long non-coding RNA PVT1 expression decreased at 24, 48, and 72 hours after transfection in the antisense LNA GapmeRs group compared to control groups. Cell viability and proliferation was significantly decreased in antisense LNA GapmeRs transfected group compared with other two groups.Conclusion: The present survey exhibited that inhibition of long non-coding PVT1 by using antisense LNA GapmeRs can dramatically reduce the proliferation of human acute erythroleukemia cells. Our results can be helpful in translational medicine for antisense therapy in acute myeloid leukemia.
Keywords
Cell proliferation
Long non-coding RNA
lncRNA PVT1
Acute myeloid leukemia
Erythroleukemia
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Journal of Isfahan Medical School
Volume 36, Issue 477 - Serial Number 477
March and April 2018
Pages 439-445

  • Receive Date 17 April 2018
  • Accept Date 06 April 2022