Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of Ultrasound Parameters on the Destruction of 4T1 Cells in the Presence of Gold Nanoparticles

Document Type : Original Article (s)

Authors
Ahmad Shanei 1
Mohammad Reza Salamat 1
Hossein Hejazi 2
Marziyeh Mirzaeiyan 3
1 Associate Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Associate Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 MSc Student, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
10.22122/jims.v36i479.10334
Abstract
Background: The ultrasound parameters such as intensity and time of radiation play an important role in cellular degradation. In this study, the effect of ultrasound radiation parameters on the degradation of 4T1 cells in the presence of gold nanoparticles was investigated.Methods: The 4T1 cells were cultured. The cells were divided into 4 groups including control, gold nanoparticle, ultrasound alone, and ultrasound at the presence of gold nanoparticle. Ultrasound irradiation were performed on the cells at intensity of 0.5, 1.0, and 1.5 W/cm2, for 1, 1.5, and 2 minutes. The viability of cells was determined using MTT assay.Findings: There was a significant difference in cell viability between the ultrasound and gold nanoparticle group and the other groups at intensity of 1.0 and 1.5 W/cm2. Moreover, there was a significant difference between the ultrasound alone and the control groups at intensity of 1.0 and 1.5 W/cm2.Conclusion: Using ultrasound in the presence of gold nanoparticles increase cell death via increasing nuclei cavitation phenomenon.
Keywords
Radiation injuries
Ultrasound radiation
Metal nanoparticles
Gold
Tumor cell line
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Journal of Isfahan Medical School
Volume 36, Issue 479 - Serial Number 479
March and April 2018
Pages 524-529

  • Receive Date 23 June 2018
  • Accept Date 06 April 2022