Journal of Isfahan Medical School

Journal of Isfahan Medical School

Attenuation of Basal Gastric Acid Secretion Following the Blockade of Hypothalamic Ventromedial D2 Receptors in Rats

Document Type : Original Article (s)

Authors
Maedeh Ghasemi 1
Nasrin Mehranfard 2
Afsaneh Eliassi 3
Hojjatallah Alaei 4
1 PhD in Physiology, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran
3 Professor, Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
10.22122/jims.v36i484.9715
Abstract
Background: Dopamine plays an important role in regulating gastric acid secretion in the central nervous system. Dopamine D2 receptors of hypothalamic ventromedial nucleus (VMH) are involved in modulation of food intake, suggesting a potential role of the VMH D2 receptor in modulating gastric acid secretion. Hence in this study, we investigated the effect of the VMH D2 receptor blockade on basal gastric acid secretion.Methods: A guide cannula was stereotaxically implanted in VMH of male Wistar rats weighing 220-250 g. The D2 receptors antagonist sulpiride (0.005, 0.050, 0.500, and 1.000 μg/0.5 μl) or saline (0.5 μl) were injected into VMH after a 5-day recovery period. Gastric acid secretion was measured using the pylorus-ligation method and titration with NaOH 0.01 N.Findings: Sulpiride at the doses of 0.050 (P < 0.010), 0.500 (P < 0.001), and 1.000 (P < 0.001) μg/0.5 μl significantly decreased basal gastric acid secretion in a dose- and time-dependent manner.Conclusion: Sulpiride at the dose of 0.500 μg/0.5 μl had maximum effect on the secretion activity of stomach. Blocking VMH D2 receptors decreases the secretion activity of stomach in rats.
Keywords
Ventromedial hypothalamic nucleus
Dopamine D2 receptors
Gastric acid
  1. Volkow ND, Wang GJ, Baler RD. Reward, dopamine and the control of food intake: implications for obesity. Trends Cogn Sci 2011; 15(1): 37-46.
  2. Strang RR. The association of gastro-duodenal ulceration and Parkinson's disease. Med J Aust 1965; 1(23): 842-3.
  3. Ozdemir V, Jamal MM, Osapay K, Jadus MR, Sandor Z, Hashemzadeh M, et al. Cosegregation of gastrointestinal ulcers and schizophrenia in a large national inpatient discharge database: revisiting the "brain-gut axis" hypothesis in ulcer pathogenesis. J Investig Med 2007; 55(6): 315-20.
  4. Rasheed N, Ahmad A, Pandey CP, Chaturvedi RK, Lohani M, Palit G. Differential response of central dopaminergic system in acute and chronic unpredictable stress models in rats. Neurochem Res 2010; 35(1): 22-32.
  5. Rasheed N, Alghasham A. Central dopaminergic system and its implications in stress-mediated neurological disorders and gastric ulcers: short review. Adv Pharmacol Sci 2012; 2012: 182671.
  6. Glavin GB. Activity of selective dopamine DA1 and DA2 agonists and antagonists on experimental gastric lesions and gastric acid secretion. J Pharmacol Exp Ther 1989; 251(2): 726-30.
  7. Glavin GB. Dopamine and gastroprotection. The brain-gut axis. Dig Dis Sci 1991; 36(12): 1670-2.
  8. Glavin GB, Dugani AM. Effects of dopamine agonists and antagonists on gastric acid secretion and stress responses in rats. Life Sci 1987; 41(11): 1397-408.
  9. Henke PG. Limbic lesions and the energizing, aversive, and inhibitory effects of non-reward in rats. Can J Psychol 1979; 33(3): 133-40.
  10. Ishikawa T, Nagata M, Osumi Y. Dual effects of electrical stimulation of ventromedial hypothalamic neurons on gastric acid secretion in rats. Am J Physiol 1983; 245(2): G265-G269.
  11. Fetissov SO, Meguid MM, Sato T, Zhang LH. Expression of dopaminergic receptors in the hypothalamus of lean and obese Zucker rats and food intake. Am J Physiol Regul Integr Comp Physiol 2002; 283(4): R905-R910.
  12. Bina KG, Cincotta AH. Dopaminergic agonists normalize elevated hypothalamic neuropeptide Y and corticotropin-releasing hormone, body weight gain, and hyperglycemia in ob/ob mice. Neuroendocrinology 2000; 71(1): 68-78.
  13. Clark JD, Gebhart GF, Gonder JC, Keeling ME, Kohn DF. The 1996 Guide for the Care and Use of Laboratory Animals. ILAR J 1997; 38(1): 41-8.
  14. Bakshi S. Considering the effect of D1- and D2- like dopaminergic receptors on food behavior in 24 hours food deprived rat [Thesis]. Tehran, Iran: Shahid Beheshti University of Medical Sciences; 2009. [In Persian].
  15. Eliassi A, Nazari M, Naghdi N. Role of the ventromedial hypothalamic orexin-1 receptors in regulation of gastric Acid secretion in conscious rats. J Neuroendocrinol 2009; 21(3): 177-82.
  16. Desai JK, Goyal RK, Parmar NS. Characterization of dopamine receptor subtypes involved in experimentally induced gastric and duodenal ulcers in rats. J Pharm Pharmacol 1999; 51(2): 187-92.
  17. Misher A, Brooks FP. Electrical stimulation of hypothalamus and gastric secretion in the albino rat. American Journal of Physiology-Legacy Content 1966; 211(2): 403-6.
  18. Salimi M, Eliassi A, Haghparast A. Intra-paraventricular nucleus microinjection of D2 receptors antagonist, sulpiride, reduces food intake in 24 hours food-deprived rats. Ir J Physiol Pharmacol 2017; 1(3): 200-193.
  19. Ghasemi T. Role of peripheral and VMH dopamine D1-like receptors on Histamin -Stimulated gastric acid secretion [Thesis]. Tehran, Iran: Shahid Beheshti University of Medical Sciences; 2004. [In Persian].
  20. Toushih M, Shahbazi F, Ghajarzadeh M, Vahedi Mazdabadi N. The Effect of Sulpiride and Bromocriptine Injection into Ventromedial Nucleus of Hypothalamus on the Volume and Acidity of Gastric Acid Secretion in the Rat. Experimental Animal Biology 2014; 3(2): 75-82. [In Persian].
  21. Yarandi SS, Hebbar G, Sauer CG, Cole CR, Ziegler TR. Diverse roles of leptin in the gastrointestinal tract: modulation of motility, absorption, growth, and inflammation. Nutrition 2011; 27(3): 269-75.
  22. Nogueiras R, Seeley RJ. Our evolving understanding of the interaction between leptin and dopamine system to regulate ingestive behaviors. Mol Metab 2012; 1(1-2): 8-9.
  23. Billes SK, Simonds SE, Cowley MA. Leptin reduces food intake via a dopamine D2 receptor-dependent mechanism. Mol Metab 2012; 1(1-2): 86-93.
  24. Kim KS, Yoon YR, Lee HJ, Yoon S, Kim SY, Shin SW, et al. Enhanced hypothalamic leptin signaling in mice lacking dopamine D2 receptors. J Biol Chem 2010; 285(12): 8905-17.
  25. ter Horst GJ, Luiten PG, Kuipers F. Descending pathways from hypothalamus to dorsal motor vagus and ambiguus nuclei in the rat. J Auton Nerv Syst 1984; 11(1): 59-75.
  26. Wu X, Gao J, Yan J, Owyang C, Li Y. Hypothalamus-brain stem circuitry responsible for vagal efferent signaling to the pancreas evoked by hypoglycemia in rat. J Neurophysiol 2004; 91(4): 1734-47.
  27. Ghasemi M, Mehranfard N, Eliassi A, Alaei HA. Modulation of the Plasma Ghrelin Level by Intra-ventromedial Hypothalamic Microinjection of D2 Receptors Agonist and Antagonist. Neuropsychiatry (London) 2018; 8(1): 89-95.
Journal of Isfahan Medical School
Volume 36, Issue 484 - Serial Number 484
May and June 2018
Pages 687-692

  • Receive Date 24 February 2018
  • Accept Date 06 April 2022