Journal of Isfahan Medical School

Journal of Isfahan Medical School

Investigating the Presence of Haemophilus Influenzae in Patients with Chronic Obstructive Pulmonary Disease (COPD) Using Molecular Method

Document Type : Original Article (s)

Authors
Hedyeh Mohaghegh-Mortazavi 1
Naghmeh Bahrami 2
Tahereh Naji 1
Abdolreza Mohamadnia 3
1 Department of Cellular and Molecular Biology, School of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2 Assistant Professor, Craniomaxillofacial Research Center AND Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3 Assistant Professor, Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD) AND Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
10.22122/jims.v37i527.11828
Abstract
Background: Chronic obstructive pulmonary disease (COPD) is a type of obstructive pulmonary disease characterized by chronic airway blockage. The aim of this study was to investigate the presence of Haemophilus influenzae in patients with COPD using molecular method.Methods: A total of 100 samples of respiratory secretion were collected from patients with COPD. The molecular identification of Haemophilus influenza was performed using specific primers against standard kits by polymerase chain reaction (PCR) method.Findings: Haemophilus influenzae bacteria were positive in 10 out of 100 patients, and 8 of 100 were positive in PCR using specific primers.Conclusion: Nucleic acid-like PCR tests, due to their sensitivity, high accuracy, and time saving, are the appropriate method for diagnosis of Haemophilus influenzae in patients with chronic pulmonary obstruction.
Keywords
Haemophilus influenzae
Chronic obstructive pulmonary disease
Molecular models
DNA Primers
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Journal of Isfahan Medical School
Volume 37, Issue 527 - Serial Number 527
March and April 2019
Pages 517-521

  • Receive Date 24 May 2019
  • Accept Date 06 April 2022