Journal of Isfahan Medical School

Journal of Isfahan Medical School

Frequency of Metallo-Beta-Lactamase and Extended- Spectrum Beta-Lactamase genes in Gram-Negative Bacteria Isolated from Ventilator- Associated Pneumonia in the Intensive Care Unit of Imam Reza Hospital in Kermanshah

Document Type : Original Article(s)

Authors
Sepide Kadivarian 1
Jale Moradi 2
Mohsen Najafi 1
Mohsen Azizi 1
Ramin Abiri 3
Sara Kooti 4
Amirhooshang Alvandi 5
1 MSc, Student Research Committee, Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Assistant Professor of Medical Bacteriology, Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Professor, Fertility and Sterility Research Center, Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
4 Assistant Professor of Medical Bacteriology, Behbahan Faculty of Medical Sciences, Behbahan, Iran
5 Associate Professor, Medical Technology Research Center, and Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
10.48305/jims.v40.i702.1085
Abstract
Background: Ventilator-associated pneumonia is the most common nosocomial infection that is responsible for increasing the mortality of patients admitted to the intensive care unit. The aim of this study was to evaluate the frequency of MBL and ESBL genes and to determine the patterns of antibiotic resistance in gram-negative bacilli isolated from ventilators in the ICU of Imam Reza Hospital in Kermanshah.
Methods: After collecting samples and identifying the bacteria by standard biochemical and microbial culture methods, 152 bacterial isolates were identified. ESBL and MBL genes were identified by phenotypic DDCT method and molecular PCR method using specific primers.
Findings: The most common microorganism isolated from ventilator-associated pneumonia (VAP) patients was Klebsiella pneumoniae (2.56%). In total, the frequency of ESBL and MBL genes was 113 (74.34%) and
8 (5.26%). The antibiotic resistance pattern showed that 36.53% of the isolates were resistant to ceftazidime, cefotaxime and imipenem. The blaSHVOS gene with a frequency of 75 (49.34%) had the highest genotypic frequency. The blaVIM gene was not found in any of the isolates.
Conclusion: The high resistance of Acinetobacter baumannii and Klebsiella pneumoniae isolates to the mentioned antibiotics and the high prevalence of ESBL-producing genes in Klebsiella pneumoniae isolates is a significant alarm in the ICU of different wards of Imam Reza Hospital. The emergence of the blaPER-1 gene in patients with VAP has raised another worry in patients with VAP in the intensive care unit.
Keywords
Ventilator-associated pneumonia
Extended Spectrum Beta-Lactamase
Metallo-Beta-Lactamase
Intensive care unit

Subjects

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Journal of Isfahan Medical School
Volume 40, Issue 702
2nd Week, March
March and April 2023
Pages 1085-1095

  • Receive Date 01 August 2022
  • Accept Date 22 February 2023