Journal of Isfahan Medical School

Journal of Isfahan Medical School

Investigating the Effect of a Combined Training Course on the Myogenesis Process Through the Decorin/Sparc Gene Pathway of Fast and Slow Twitch Fibers of Immature Male Rats

Document Type : Original Article (s)

Authors
Mohammad Reza Khalili Far 1
Mohammad Reza Fadaei Chafy 2
Alireza Elmieh 3
1 PhD Candidate of Exercise Physiology, Department of Physical Education and Sport Science, Rasht Branch, Islamic Azad University, Rasht, Iran
2 Assistant Professor, Department of Physical Education and Sport Science, Rasht Branch, Islamic Azad University, Rasht, Iran
3 Associate Professor, Department of Physical Education and Sport Science, Rasht Branch, Islamic Azad University, Rasht, Iran
10.48305/jims.v42.i779.0696
Abstract
Background: Skeletal muscle is a major endocrine organ that releases myokines and plays a pivotal role in the communication between muscle and other tissues. The present study aimed to determine the effect of a combined training course on the myogenesis process through the pathway of decorin and spark genes in the muscles of immature rats.
Methods: In the present study, ten two-week-old male rats were randomly divided into two control and training groups. The resistance-aerobic training program was carried out for 6 weeks on separate days. The rats were dissected 48 hours after the last training session, and the gastrocnemius and soleus muscles were separated and used for pathological tests.
Findings: Based on statistical analysis, it was found that in the training group, the expression of Spark gene (P ≤ 0.001) and decorin (P ≤ 0.001) in the soleus muscle and the expression of Spark gene (P ≤ 0.028) and decorin (P ≤ 0.001) in the gastrocnemius muscle had higher values than the control group. However, the expression values of Spark (P ≤ 0.015) and decorin (P ≤ 0.001) genes in the soleus muscle were significantly higher compared to the calf muscle.
Conclusion: The results showed that myogenesis increased due to resistance-aerobic training in immature male rats, which was associated with increased expression of the decorin spark gene in both slow and fast-twitch muscle fibers. However, the expression of both spark and decorin genes in the slow-twitch fiber had higher values than the fast-twitch fibers, which indicates that this type of muscle fiber is more trainable.

Highlights

Mohammad Reza Fadai Chafi: Google Scholar 

Alireza Elmia: Google Scholar

Keywords
Resistance training
Exercise
Skeletal muscle
Muscle development
Osteonectin
Decorin
Subjects
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Journal of Isfahan Medical School
Volume 42, Issue 779
3rd Week, October
September and October 2024
Pages 696-702

  • Receive Date 25 April 2024
  • Accept Date 06 October 2024