The effect of 8 weeks of resistance training combined with the use of Iso Whey Isolate supplementation on serum levels of Activin A, Decorin, and TGF-β in middle-aged men

Shekarian, Motahereh; Shabaninia, Meysam; Rajabi, Hamid; Sajjadian, Mohammad

doi:10.48305/jims.2026.46067.2822

The effect of 8 weeks of resistance training combined with the use of Iso Whey Isolate supplementation on serum levels of Activin A, Decorin, and TGF-β in middle-aged men

Articles in Press

Document Type : Original Article(s)

Authors

Motahereh Shekarian ¹

meysam shabaninia ¹

Hamid Rajabi ²

Mohammad Sajjadian ¹

¹ Department of Physical education and Sport Science, Sho.C., Islamic Azad University, Shoushtar, Iran

² Department of Exercise Physiology, Kharazmi University, Tehran, Iran

10.48305/jims.2026.46067.2822

Abstract

Introduction: Skeletal muscle is known as an organ that can produce molecules with vital functions. The aim of this study was to determine the effect of 8 weeks of resistance training and IsoVie supplementation on the levels of activin A, decorin, and TGF-β in middle-aged men.

Methods: The study was conducted as a quasi-experimental and applied study. The statistical population included sedentary men aged 40 to 45 years in Bandar Mahshahr. 40 people were purposively selected and divided into four groups: resistance training, supplementation, exercise + supplementation, and control (10 people in each group). Resistance training was performed for 8 weeks, three sessions per week, with an intensity of 60 to 70% of one repetition maximum. A 25-gram whey protein isolate supplement was consumed twice a day after breakfast and lunch. The data were analyzed using a two-way ANOVA test at a significance level of 0.05.

Findings: In the exercise group (P=0.001), supplement (P=0.01) and exercise + supplement (P=0.001), the amount of activin A significantly decreased. . In the exercise group (P=0.001), supplement (P=0.01) and exercise + supplement (P=0.001), the amount of activin A significantly decreased. In the exercise group (P=0.003) and exercise + supplement (P=0.001), the amount of TGF-β significantly decreased. Conclusion: Exercise alone and in interaction with whey protein has a better effect in improving myokines effective in muscle hypertrophy.

Keywords

Myokine

resistance training

whey protein

muscle growth regulation

Subjects