Journal of Isfahan Medical School

Journal of Isfahan Medical School

Regulation of Pgc1α/mt-Nd1/mt-Nd5/Atp5a1 gene expression and ATP concentration in skeletal muscle in mice with type 2 diabetes: The role Hippophae rhamnoides extract and metformin as complementary medicine.

Document Type : Original Article(s)

Authors
Kamran Safavi 1
Fatemeh Hajibabaie 2
Navid Abedpoor 3
1 Department of Plant Biotechnology, Medicinal Plants Research Centre, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 Department of Physiology, Medicinal Plants Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, AND Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 Department of Physiology, Medicinal Plants Research Center, AND Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
10.48305/jims.v43.i847.1966
Abstract
Introduction: Mitochondria play a key role in maintaining the homeostasis of cell metabolism. Mitochondrial dysfunction and oxidative phosphorylation disorders play an important role in the pathogenesis of diabetic skeletal muscle. In this regard, the aim of this study is to investigate the effect of type 2 diabetes on skeletal muscle mitochondrial markers and the use of Hippophae rhamnoides extract with metformin as a complementary medicine. Methods: 30 C57BL/6 mice with 6-7 week-olds were divided into five groups: control group, Type 2 diabetes group, 3. The group with type 2 diabetes was treated with metformin, Type 2 diabetes group treated with Hippophae rhamnoides extract, Type 2 diabetes group treated with metformin and Hippophae rhamnoides extract. The genes obtained from bioinformatics analysis. To investigation gene expression, the Real-Time PCR technique was used, and muscle ATP concentration was assesd using the ELISA method. Findings: In the diabetes group, compared to the control group, the expression of mt-Nd5/PGC1α/ATP5/mt-Nd1 genes and ATP concentration decreased (p<0.001). Consumption of Hippophae rhamnoides extract and metformin separately increased the expression level of mt-Nd5/PGC1α/ATP5/mt-Nd1 genes and ATP concentration (p<0.001). Simultaneous consumption of Hippophae rhamnoides extract and metformin significantly increased the expression of mt-Nd5/PGC1α/ATP5/mt-Nd1 genes and ATP concentration (p<0.001). Conclusion: Hippophae rhamnoides extract and metformin can regulate mt-Nd5/PGC1α/ATP5/mt-Nd1 network and ATP concentration in muscle tissue.

Highlights

Kamran Safavi:Google Scholar, PubMed

Fatemeh Hajibabaie:Google Scholar, PubMed

Navid Abedpoor :Google Scholar, PubMed

Keywords
Hippophae rhamnoides
Pgc1&‌alpha؛
Complementary Therapies
metformin
Computational Biology &‌emsp

Subjects

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Journal of Isfahan Medical School
Volume 43, Issue 847
3rd Week, March
March and April 2026
Pages 1966-1977

  • Receive Date 27 November 2024
  • Accept Date 10 December 2025