Establishment of Experimental Autoimmune Encephalitis Model by Injection of Different Combination of Homogenized Brain Tissue of Rat with Complete Freund's Adjuvant

Document Type : Original Article (s)

Authors

1 Student of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Assistant Professor, Physiology Research Center and Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Experimental autoimmune encephalitis (EAE) is an animal model of multiple sclerosis (MS). Today in Iran for induction of EAE on rats we need to buy specific encephalitogen such as myelin basic protein (MBP), myelin oligodendrocyte glyco protein (MOG), Proteo lipo protein (PLP) from developed countries. It is very essential for us to find a way for induction of EAE which is cheaper and more rapid that make us independent. It is a fundamental technique.because if we want to study MS, we will need animal model. If we get proper result, we can over come many obstacles and difficulties in future for further investigations on MS and its etiology and its pathophysiological mechanism.Methods: In this study after one of rats exsanguinations the brain tissue was put in a saline solution and then was homogenized with a hemogenizer of Silent Crusher S model. It brought a suspension. A constant amount of Complete Freund's adjuvant (CFA) with different amounts of quantity of this prepared suspension then was combined and was injected subcutaneously to a series of similar rats n = 10. In this investigation all were rats and 590±  10 gr and 3 month old. The administration was repeated with a week interval for some of the rats and the results were compared. Also different combinations were administered and the results were compared. Finally neurological deficit was looked for which was our aim.Findings: This study illustrated that supernatant solution was more encephalitogen than Suspension solution, and this was usable in an applicable technique for induction of Neurological paralysis in rats (P < 0.05) but dose not show any optimum combination of supernatant and CFA for that aim.Conclusion: With modifying old method for induction of EAE we could find preferable one rather than high costly methods which are used today. Also this method should be assessed with further investigations on different mice and rats strains and compared with each other and also with further investigations this method should be compared with other methods in literature. 

Keywords

References

  1. Guo L, Li Y, Lin H, Ji X, Li J, Que L, et al. Evaluation of a rat model of experimental autoimmune encephalomyelitis with human MBP as antigen. Cell Mol Immunol 2004; 1(5): 387-91.
  2. Racke MK, Critchfield JM, Quigley L, Cannella B, Raine CS, McFarland HF, et al. Intravenous antigen administration as a therapy for autoimmune demyelinating disease. Ann Neurol 1996; 39(1): 46-56.
  3. Abbas A, Lichtman AH. Cellular and Molecular Immunology. 5th ed. Philadelphia: Saunders; 2003. p. 422.
  4. Tafreshi AP, Mostafavi H, Zeynali B. Induction of experimental allergic encephalomyelitis in C57/BL6 Mice: an animal model for multiple sclerosis. Iran J Allergy Asthma Immunol 2005; 4(3): 113-7.
  5. Pietropaolo M, Olson CD, Reiseter BS, Kasaian MT, Happ MP. Intratracheal administration to the lung enhances therapeutic benefit of an MBP peptide in the treatment of murine experimental autoimmune encephalomyelitis. Clin Immunol 2000; 95(2): 104-16.
  6. Jones RE, Bourdette D, Moes N, Vandenbark A, Zamora A, Offner H. Epitope spreading is not required for relapses in experimental autoimmune encephalomyelitis. J Immunol 2003; 170(4): 1690-8.
  7. Longatti PL, Canova G, Guida F, Carniato A, Moro M, Carteri A. The CSF myelin basic protein: a reliable marker of actual cerebral damage in hydrocephalus. J Neurosurg Sci 1993; 37(2): 87-90.
  8. Longatti PL, Guida F, Agostini S, Carniato B, Carteri A. The CSF myelin basic protein in pediatric hydrocephalus. Childs Nerv Syst 1994; 10(2): 96-8.
  9. Harling-Berg CJ, Knopf PM, Cserr HF. Myelin basic protein infused into cerebrospinal fluid suppresses experimental autoimmune encephalomyelitis. J Neuroimmunol 1991; 35 (1-3): 45-51.
  10. Takahashi T. [Monoamines, monoamine metabolites, neuron specific enolase and myelin basic protein concentrations in cerebrospinal fluid of resuscitated patients]. Nihon Shinkei Seishin Yakurigaku Zasshi 1997; 17(1): 7-16.
  11. Berger RP, Dulani T, Adelson PD, Leventhal JM, Richichi R, Kochanek PM. Identification of inflicted traumatic brain injury in well-appearing infants using serum and cerebrospinal markers: a possible screening tool. Pediatrics 2006; 117(2): 325-32.
Journal of Isfahan Medical School
Volume 29, Issue 171 - Serial Number 171
November and December 2012
Pages 2732-2738

Files

History

  • Receive Date: 01 November 2011
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics